LIN-28A Antibody

Name: LIN-28A Antibody
Brand: Novus Biologicals
SKU: NBP2-19355
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-19355

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIN-28A. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus; nucleolus

Marker

Undifferentiated human embryonic stem cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit LIN-28A Antibody (NBP2-19355) is a polyclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LIN-28A Antibody

Western Blot: LIN-28A Antibody [NBP2-19355]

Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: LIN-28A Antibody [NBP2-19355]

Immunohistochemistry-Paraffin: LIN-28A Antibody [NBP2-19355] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Flow Cytometry: LIN-28A Antibody [NBP2-19355]

Flow Cytometry: LIN-28A Antibody [NBP2-19355] - Human embryonic stem cells, staining with LIN28 antibody at 1:100 dilution (purple) or rabbit IgG (black).

Western Blot: LIN-28A Antibody [NBP2-19355] -

Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate)
A: hESC
12% SDS PAGE
NBP2-19355 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for LIN-28A Antibody

Application

Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIN-28A

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

Long Name

RNA-binding Protein LIN-28

Alternate Names

CSDD1, LIN28, LIN28A, Tex17, ZCCHC1

Gene Symbol

LIN28A

UniProt

Q9H9Z2

Additional LIN-28A Products

Product Documents for LIN-28A Antibody

Download Product Datasheets

Download SDS

Product Specific Notices for LIN-28A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Translation Regulation

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