Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 103 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Max (Accession#: NP_002373; Met1-Ser160).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Max Antibody (103) - Azide and BSA Free

Western Blot: Max Antibody (103) [NBP2-90153]

Western Blot: Max Antibody (103) [NBP2-90153]

Western Blot: Max Antibody (103) [NBP2-90153] - Lane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:20 kDa(We are unsure as to the identity of these extra bands.)
Flow Cytometry: Max Antibody (103) [NBP2-90153]

Flow Cytometry: Max Antibody (103) [NBP2-90153]

Flow Cytometry: Max Antibody (103) [NBP2-90153] - Analysis of Human MAX expression on Raji cells stained with purified anti-Human MAX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation: Max Antibody (103) [NBP2-90153]

Immunoprecipitation: Max Antibody (103) [NBP2-90153]

Immunoprecipitation: Max Antibody (103) [NBP2-90153] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-MAX rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAX rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa
Max Antibody (103) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Max Antibody (103) [NBP2-90153] -

Immunocytochemistry/Immunofluorescence: Max Antibody (103) [NBP2-90153] - Staining of Human MAX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90153 antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Applications for Max Antibody (103) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Flow Cytometry

1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Max

MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD - regulating gene expression.

Long Name

Myc associated factor X

Alternate Names

BHLHD4, bHLHd4MGC11225, bHLHd5, bHLHd6, bHLHd7, bHLHd8, Class D basic helix-loop-helix protein 4, helix-loop-helix zipper protein, MAX protein, MGC10775, MGC18164, MGC34679, MGC36767, MYC associated factor X, Myc-associated factor X, orf1, protein max

Gene Symbol

MAX

Additional Max Products

Product Documents for Max Antibody (103) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Max Antibody (103) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Max Antibody (103) - Azide and BSA Free

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Protocols

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FAQs

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