Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Novus Biologicals | Catalog # NB300-690

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Mer Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: corresponding to amino acids 746-757 (CKKIYSGDYYRQG-amide) [p Y749, p Y753, p Y754] of Human MERTK.

Modification

p Tyr754, p Tyr749, p Tyr753

Specificity

MERTK [Tyr749/Tyr753/Tyr754]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Mer [p Tyr754, p Tyr749, p Tyr753] Antibody (NB300-690) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Mer Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Western Blot: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Western Blot: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Western Blot: Mer [p Tyr749, p Tyr753] Antibody [NB300-690] - Phospho & non-Phospho-MerTK with Phospho-MERTK Antibody (NB300-690). Phosphorylated (1) & un-phosphorylated (2, 3) MERTK was immunoprecipitated with MERTK and blotted with Phospho-MerTK antibody (NB300-690). MW of MERTK is 174 kDa. Lower broad band is of IgGs in the immunoprecipitates.
Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Mer-[p-Tyr754--p-Tyr749--p-Tyr753]-Antibody-Immunocytochemistry-Immunofluorescence-NB300-690-img0005.jpg
Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Immunocytochemistry/ Immunofluorescence: Mer [p Tyr754, p Tyr749, p Tyr753] Antibody [NB300-690]

Mer-[p-Tyr754--p-Tyr749--p-Tyr753]-Antibody-Immunocytochemistry-Immunofluorescence-NB300-690-img0004.jpg

Applications for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:250

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:750

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mer

MerTK (c-mer, Nyk, Eyk) is a tyrosine kinase that belongs to a family of transmembrane receptors (1). MerTk has been implicated in reversible cell growth arrest, survival, proliferation and cell adhesion. Growth arrest specific protein 6 (Gas6) is an activating ligand for MerTK (2-3). MerTK is required for clearance of apoptotic cells by mononuclear phagocytes in mice; with its absence resulting in progressive lupus like autoimmunity (4). Defects in MERTK are a cause of retinitis pigmentosa (RP) (5).

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human)

Gene Symbol

MERTK

UniProt

Q12866

Additional Mer Products

Product Documents for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

Certificate of Analysis

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Product Specific Notices for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mer [p Tyr754, p Tyr749, p Tyr753] Antibody

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    • A: The antibody stabilization buffer contains Tris-Glycine buffer pH7.4, glycerol, stabilizing protein BSA, preservative Sodium azide at 0.02% final concentration; however, the exact composition of this buffer is proprietary.
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