Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, In Situ Hybridization, CyTOF-reported

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2449D
View all CD68/SR-D1 Primary Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived mouse CD68
Asp21-Pro282
Accession # P31996

Specificity

Detects mouse CD68 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CD68/SR‑D1 Antibody

Detection of CD68 in Mouse Liver via seqIF™ staining on COMET™​

CD68 was detected in immersion fixed paraffin-embedded sections of mouse Liver using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog #MAB101141) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of Kupffer cells. Protocol available in COMET™ Panel Builder.​​

Detection of CD68 in Mouse Brain Medulla via seqIF™ staining on COMET™​

CD68 was detected in perfusion fixed-frozen sections of mouse Brain Medulla using Rabbit Anti-Mouse CD68, Monoclonal Antibody (Catalog#MAB101141) at 20ug/mL at 27° Celsius for 8minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5 minutes at room temperature followed by a 20-minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

CD68/SR-D1 antibody in Mouse Liver by Immunohistochemistry (IHC-P).

CD68/SR‑D1 in Mouse Liver.

CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD68 antibody in Raw264.7 Mouse Cell line antibody by Flow Cytometry.

Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry.

Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.

Applications for Mouse CD68/SR‑D1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse liver

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: RAW264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with ice-cold methanol

Multiplex Immunofluorescence

5-20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse liver and perfusion fixed-frozen sections of mouse brain medulla

Reviewed Applications

Read 3 reviews rated 5 using MAB101141 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD68/SR-D1

Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein.  It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells.  Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets.  Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol

CD68

UniProt

P31996

Additional CD68/SR-D1 Products

Product Documents for Mouse CD68/SR‑D1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD68/SR‑D1 Antibody

For research use only

Citations for Mouse CD68/SR‑D1 Antibody

Customer Reviews for Mouse CD68/SR‑D1 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
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3 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Mouse CD68/SR-D1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 06/23/2022
    Deparaffin; Citrate acid (6.0) 30min while steaming; 1:100. Works very well! :)
    Mouse CD68/SR‑D1 Antibody MAB101141
  • Mouse CD68/SR-D1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 03/09/2022
    Mouse CD68/SR‑D1 Antibody MAB101141
  • Mouse CD68/SR-D1 Antibody
    Name: Tina Yuan
    Application: Immunohistochemistry
    Sample Tested: mouse oral tissue
    Species: Mouse
    Verified Customer | Posted 11/23/2020
    Mouse CD68/SR‑D1 Antibody MAB101141

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Protocols

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