EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
Mouse EMMPRIN/CD147 Antibody [Biotin]
R&D Systems | Catalog # BAF772
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Western Blot, Flow Cytometry
Cited:
Immunohistochemistry, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652
Ala22-Arg209
Accession # NP_001070652
Specificity
Detects mouse EMMPRIN/CD147 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human EMMPRIN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse EMMPRIN/CD147 Antibody [Biotin]
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse EMMPRIN/CD147 Antibody [Biotin]
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse thymocytes
Sample: Mouse thymocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: EMMPRIN/CD147
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
UniProt
Additional EMMPRIN/CD147 Products
Product Documents for Mouse EMMPRIN/CD147 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse EMMPRIN/CD147 Antibody [Biotin]
For research use only
Citations for Mouse EMMPRIN/CD147 Antibody [Biotin]
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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