Mouse Endoglycan/PODXL2 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3534X Datasheet / COA / SDS

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AF3534X-100UG

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Mouse Endoglycan/PODXL2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Gly30-Gly498
Accession # Q8CAE9

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% amino acid sequence identity with human and rat endoglycan, respectively.

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Alternate Names

Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2