Mouse Endoglycan/PODXL2 Antibody

R&D Systems | Catalog # AF3534

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Endoglycan/PODXL2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Gly30-Gly498
Accession # Q8CAE9

Specificity

Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Endoglycan/PODXL2 Antibody

Endoglycan/PODXL2 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

Endoglycan/PODXL2 in Mouse Testis.

Endoglycan/PODXL2 was detected in perfusion fixed frozen sections of mouse testis using 1.7 µg/mL Goat Anti-Mouse Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Endoglycan/PODXL2 by Immunohistochemistry

Detection of Endoglycan/PODXL2 by Immunohistochemistry

Expression of CD99 and PODXL2 in porcine testis. (A) Immunohistochemical staining of CD99 and PODXL2 in porcine testis. Scale bars = 50 μm. Coimmunofluorescence examination of the expression of (B) CD99 with UCHL1 and (C) PODXL2 with c-KIT in porcine testis. Scale bars = 50 μm. Data are normalized by the number of double-positive cells for three independent experiments (D) CD99 with UCHL1 and (E) PODXL2 with c-KIT Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34872612), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Endoglycan/PODXL2 by Immunohistochemistry

Detection of Endoglycan/PODXL2 by Immunohistochemistry

Single-cell porcine spermatogonia trajectories reveal transitions with differentiation and initiation of meiosis. (A) Focused analysis (t-SNE and clustering) of the mitosis and initiation of meiotic germ cells reveals differentiation progression of spermatogonia. (B) t-SNE plot reveal the states in spermatogonia differentiation. (C) Dot plot depicts the expression of marker genes in each state. The dot size represents the percentage of cells expressed and the color intensity represents relative expression level. (D) The correlation across each state and illustrated by Pearson correlation analysis. (E) Expression patterns of new defined marker genes for undifferentiated and differentiating spermatogonia visualized in t-SNE plots. (F) Heatmap of marker genes from each state. Top, cell types; Left, number of marker genes; Right, representative GO terms and representative genes Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34872612), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Endoglycan/PODXL2 by Immunohistochemistry

Detection of Endoglycan/PODXL2 by Immunohistochemistry

Expression of CD99 and PODXL2 in porcine testis. (A) Immunohistochemical staining of CD99 and PODXL2 in porcine testis. Scale bars = 50 μm. Coimmunofluorescence examination of the expression of (B) CD99 with UCHL1 and (C) PODXL2 with c-KIT in porcine testis. Scale bars = 50 μm. Data are normalized by the number of double-positive cells for three independent experiments (D) CD99 with UCHL1 and (E) PODXL2 with c-KIT Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34872612), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Endoglycan/PODXL2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Endoglycan/PODXL2

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endoglycan/PODXL2

Endoglycan, also named podocalyxin-like 2 protein, is a type I transmembrane glycoprotein that belongs to the CD34/podocalyxin family of sialomucins that are expressed on endothelial cells and hematopoietic precursors, vascular smooth muscle cells, and some leukocyte subpopulations. The extracellular domain of Endoglycan contains an N-terminal acidic domain with several polyglutamate tracts, a mucin-like domain and a membrane proximal globular domain. Unlike other sialomucins, Endoglycan contains chondroitin sulfate modifications. Within the extracellular domain, mouse endoglycan shares 73% and 96% amino acid sequence identity with human and rat endoglycan, respectively.

Alternate Names

PODLX2, PODXL2

Entrez Gene IDs

50512 (Human); 319655 (Mouse)

Gene Symbol

PODXL2

UniProt

Q8CAE9

Additional Endoglycan/PODXL2 Products

Product Documents for Mouse Endoglycan/PODXL2 Antibody

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Product Specific Notices for Mouse Endoglycan/PODXL2 Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Endoglycan/PODXL2 Antibody

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Protocols

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