The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.
Mouse TER-119 Antibody (TER-119)
R&D Systems | Catalog # MAB1125
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-reported
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # TER-119
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Product Specifications
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse TER-119 Antibody (TER-119)
Detection of TER-119 Erythroid Antigen by Immunohistochemistry
P12 suppressed retinal neovascularization and vascular leakage in the OIR mouse model. (A) Schematic depiction of intravitreal P12 treatment in OIR pups. (B-D) P12 markedly decreased the retinal neovascularization and avascular area in the retina of OIR mice. (E) Gross examination of retinas isolated from OIR eye-cups. Black arrowhead depicts the area of vascular leakage (blood island). Scale bar = 500 μm.(F) Quantification of the area of blood island in PBS and P12 treated retinas. (G) Immunostaining of the erythrocyte marker TER119 in retinal flatmount of PBS and P12 treated mice with OIR. (H) Quantification of retinal vascular leakage by calculating the area of extravascular TER119+ cells in retinal flatmount. (I, J) P12 markedly decreased the degree of FITC- dextran leakage in the retina of OIR mice. Scale bar = 300 μm. Image collected and cropped by CiteAb from the following open publication (https://www.thno.org/v15p3943.htm), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TER-119 Erythroid Antigen by Immunohistochemistry
TCF19‐silenced tumors show vessel normalization with reduced vascular permeability. (A–C) Analysis of the indicated proteins by immunofluorescence in shScramble and shTCF19 tumors from the in vivo orthotopic experiment. CD31 and alpha SMA (A). B‐catenin (B). TER119 (C). The tumors selected for the IHC/IF analysis are indicated in Fig. S7 (n = 6 tumors per group). A nested t‐test was performed for statistical analysis (left panels). Error bars represent SEM. Scale bar: 500 μm and 50 μm (A and B), and 50 μm (C). Representative images are shown (right panels). P, P‐value. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40952912), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse TER-119 Antibody (TER-119)
Application
Recommended Usage
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow
Sample: Mouse bone marrow
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic liver (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryonic liver (13 d.p.c.)
Immunoprecipitation
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Western Blot
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Reviewed Applications
Read 2 reviews rated 5 using MAB1125 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TER-119
Additional TER-119 Products
Product Documents for Mouse TER-119 Antibody (TER-119)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TER-119 Antibody (TER-119)
For research use only
Related Research Areas
Citations for Mouse TER-119 Antibody (TER-119)
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Customer Reviews for Mouse TER-119 Antibody (TER-119) (2)
5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: Erythroid CellsSpecies: MouseVerified Customer | Posted 09/08/2021
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Application: Immunohistochemistry-FrozenSample Tested: Embryonic spinal cordSpecies: MouseVerified Customer | Posted 05/09/2019
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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