Mouse TNF RII/TNFRSF1B Antibody

Name: Mouse TNF RII/TNFRSF1B Antibody
Brand: R&D Systems
SKU: AF-426-PB
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # AF-426-PB

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Citations (18)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TNF RII/TNFRSF1B
Val23-Gly258
Accession # P25119

Specificity

Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TNF RII/TNFRSF1B Antibody

Detection of Mouse TNF RII/TNFRSF1B by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TNF RII/TNFRSF1B in Raw264.7 Mouse Cell Line by Flow Cytometry.

Raw264.7 mouse macrophage cell line, resting (open histogram) or treated with 1 μg/mL LPS overnight (filled histogram), was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry.

L-929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

TNF RII/TNFRSF1B in L‑929 Mouse Cell Line.

TNF RII/TNFRSF1B was detected in immersion fixed L‑929 mouse fibroblast cell line using Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse TNF RII / TNFRSF1B ELISA Standard Curve

Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 426-R2) was serially diluted and captured by Hamster Anti-Mouse TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB426) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

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Applications for Mouse TNF RII/TNFRSF1B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB4263).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse sTNF RII/TNFRSF1B DuoSet ELISA Kit (Catalog # DY426) for convenient development of a sandwich ELISA or the Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit (Catalog # MRT20) for a complete optimized ELISA.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Raw264.7 mouse macrophage cell line treated with LPS and L‑929 mouse fibroblast cell line

Immunocytochemistry

1.7-15 µg/mL
Sample: Immersion fixed L‑929 mouse fibroblast cell line

Western Blot

0.2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.