Nanog Antibody (23D2-3C6)

Western Blot: Nanog Antibody (23D2-3C6) [NBP2-22632]

Western Blot: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of 60 ug of NCCIT nuclear and cytoplasmic fractions lysates and 10ul of PageRuler Prestained Protein Ladder

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632]

Immunocytochemistry/Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (green) in NTERA-2 and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632]

Immunocytochemistry/Immunofluorescence: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.

Flow Cytometry: Nanog Antibody (23D2-3C6) [NBP2-22632]

Flow Cytometry: Nanog Antibody (23D2-3C6) [NBP2-22632] - Analysis of Nanog (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Nanog monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.