Nestin Antibody (4D11) - BSA Free

Novus Biologicals | Catalog # NBP1-92717

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D11

Format

BSA Free
View all Nestin Primary Antibodies »

Product Specifications

Immunogen

Recombinant construct, amino acids 317-630 of the human Nestin protein expressed in and purified from E. coli. [UniProt P48681]

Marker

Neural Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nestin Antibody (4D11) - BSA Free

Western Blot: Nestin Antibody (4D11) [NBP1-92717]

Western Blot: Nestin Antibody (4D11) [NBP1-92717]

Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717]

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717]

Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717]

Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). IHC image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717]

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for Vimentin and so appear red. Others contain Vimentin and not Nestin and so appear green - these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish.

Applications for Nestin Antibody (4D11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:5000
Application Notes
This Nestin (4D11) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 240 kDa. IHC reported in verified customer review. Use in FLOW reported in scientific literature (PMID: 31203166).

Reviewed Applications

Read 1 review rated 5 using NBP1-92717 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nestin

Nestin (NES) is a class VI intermediate filament protein which is unable to self-assemble or form homodimers because of a short NH2-terminus and is essential for the development of brain, eye etc. as well as for processes such as survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. It facilitates the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and the trafficking/distribution of IF proteins or other factors to daughter cells during progenitor cell division. Nestin is expressed in dividing cells during early stages of development e.g. CNS, PNS, myogenic or other tissues etc, whereas with differentiation, it is downregulated followed by replacement with tissue-specific intermediate filament proteins, and therefore, is widely used as a neuronal stem cell marker. Under pathological conditions, nestin is expressed in repair processes in CNS, muscle, liver, and infarcted myocardium, and in tumors, increased nestin correlates with aggressive growth, metastasis, and poor prognosis. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers, indicating nestin's use as a marker for newly synthesized tumor vessels.

Alternate Names

NES

Entrez Gene IDs

10763 (Human)

Gene Symbol

NES

UniProt

P48681

Additional Nestin Products

Product Documents for Nestin Antibody (4D11) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nestin Antibody (4D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nestin Antibody (4D11) - BSA Free

Customer Reviews for Nestin Antibody (4D11) - BSA Free (1)

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  • Nestin Antibody (4D11)
    Name: Anonymous
    Application: Immunohistochemistry-microslice section
    Sample Tested: Mouse cortex
    Species: Mouse
    Verified Customer | Posted 07/27/2017
    Mouse cortex microslice section (from postnatal day 3) was immunostained with Nestin Antibody (4D11)(1:250 dil.).
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