Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

Novus Biologicals | Catalog # NBP2-61679

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B6G8

Format

BSA Free
View all Nicotinic Acetylcholine R alpha 6/CHRNA6 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 6/CHRNA6 (AA: 26-239) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)BSA Free [NBP2-61679]

Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)BSA Free [NBP2-61679]

Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Immunohistochemistry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.
Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)BSA Free [NBP2-61679]

Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8)BSA Free [NBP2-61679]

Western Blot: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

Flow Cytometry: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).
ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free [NBP2-61679]

ELISA: Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) [NBP2-61679] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 6/CHRNA6

This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence.

Long Name

Nicotinic Acetylcholine Receptor alpha 6

Alternate Names

CHRNA6, Nicotinic Acetylcholine Ra6

Gene Symbol

CHRNA6

Additional Nicotinic Acetylcholine R alpha 6/CHRNA6 Products

Product Documents for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free

There are currently no reviews for this product. Be the first to review Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free and earn rewards!

Have you used Nicotinic Acetylcholine R alpha 6/CHRNA6 Antibody (5B6G8) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies