non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13532

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (99%), Chicken (95%), Rat (97%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT218

Format

Azide and BSA Free
View all Non-muscle Myosin IIA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Centrifuge briefly prior to opening.

Scientific Data Images for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
non-muscle Myosin IIA Antibody (GT218)

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
non-muscle Myosin IIA Antibody (GT218)

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
non-muscle Myosin IIA Antibody (GT218)

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:500.
Blue: Fluoroshield with DAPI.
non-muscle Myosin IIA Antibody (GT218)

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
non-muscle Myosin IIA Antibody (GT218)

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -

Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT218] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Non-muscle Myosin IIA

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Gene Symbol

MYH9

Additional Non-muscle Myosin IIA Products

Product Documents for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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