Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Novus Biologicals | Catalog # NBP1-18961

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # MIC1-1C3

Format

BSA Free
View all Oval Cell Marker Primary Antibodies »

Product Specifications

Immunogen

Nonparenchymal cells from DDC-treated mice

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30236451).

Localization

Cell surface

Marker

Hepatic periductal cell Marker

Specificity

Specific for hepatic proliferating duct cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) - BSA Free [NBP1-18961]

Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) - BSA Free [NBP1-18961]

Immunohistochemistry-Frozen: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Ductular reaction in injured mouse liver. MIC1-1C3 (red) and DAPI (blue). IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: Oval Cell Marker Antibody (MIC1-1C3) - BSA Free [NBP1-18961]

Immunohistochemistry: Oval Cell Marker Antibody (MIC1-1C3) - BSA Free [NBP1-18961]

Immunohistochemistry: Oval Cell Marker Antibody (MIC1-1C3) [NBP1-18961] - Analysis of DDC treated mouse liver.

Applications for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50 - 1:100

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

reported in scientific literature
Application Notes
This Oval Cell Marker (MIC1-1C3) antibody is useful for Immunohistochemistry on acetone fixed frozen sections and Flow cytometry. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-18961 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Oval Cell Marker

Liver is well known to possess facultative stem cells which can be activated in response to injury and hepatic stem cell activation was first described in rat model, wherein, chemical damage in combination with partial hepatectomy resulted in the emergence of a new cell type which was described according to its nuclear morphology as "oval cells". The word 'oval cells' is now employed to define population of small proliferating cells with oval-shaped nuclei which arise within liver tissue following certain types of hepatic injury. Oval cell proliferation is activated when the proliferative capacity of hepatocytes is impaired, as well as in certain models of carcinogenesis. Histologically, hepatic tissue is highly complex and contains many different cell types including various kinds of hepatocytes, ducts, hematopoietic cells, endothelial cells, macrophages, stellate cells etc., and the oval cells have been proposed to be bipotential progenitor cells capable of generating both hepatocytes and bile ducts. Due to their bipotency and high clonogenicity, oval cells have been considered putative liver stem cells and research is in progress for elucidation of signaling molecules involved in controlling the activation, proliferation and differentiation of this unique cell population. Our oval cell antibodies have been developed against hepatic nonparenchymal cells (NPCs) from mice undergoing oval cell activation and with these antibodies, it is now possible to perform microarray analysis of distinct liver cell populations during induced oval cell response or certain pathological conditions.

Additional Oval Cell Marker Products

Product Documents for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

Customer Reviews for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free (2)

5 out of 5
2 Customer Ratings
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Customer Images

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  • Oval Cell Marker Antibody (MIC1-1C3)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 06/04/2021
    Ductular reaction in injured mouse liver. MIC1-1C3 (red) and DAPI (blue).
    Acetone fixation
    Oval Cell Marker Antibody (MIC1-1C3) - BSA Free NBP1-18961
  • Oval Cell Marker Antibody (MIC1-1C3)
    Name: Bin Li
    Application: Flow Cytometry
    Sample Tested: mouse liver NPC
    Species: Mouse
    Verified Customer | Posted 12/06/2016

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Protocols

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FAQs for Oval Cell Marker Antibody (MIC1-1C3) - BSA Free

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  • Q: What is the recommended antigen retrieval conditions for IHC(P)?

    A: The validation for IHC in paraffin embedded sections came from the following publication: NCBI site. In this publication, the authors used 3mm thick paraffin embedded tissue sections smeared on a slide fixed with methanol at 4 degrees C for 5 minutes. Therefore, we do not have a recommended antigen retrieval method. If you are trying to stain in formalin or paraformaldehyde fixed sections, I would recommend starting with either citrate buffer pH 6.0 or proteinase K for antigen retrieval.

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