Parkin Antibody (JF82-09)

Novus Biologicals | Catalog # NBP2-67017

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF82-09 expressed in HEK293
View all Parkin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within N-terminal human Parkin. (SwissProt: O60260 Human; SwissProt: Q9WVS6 Mouse; SwissProt: Q9JK66 Rat)

Localization

Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Parkin Antibody (JF82-09)

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.
Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017]

Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017]

Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/ Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017]

Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017]

Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.
Parkin Antibody (JF82-09)

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Parkin Antibody (JF82-09) Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western Blot: Parkin Antibody (JF82-09) [NBP2-67017]

Western blot analysis on different lysates with NBP2-67017 at 1/500 dilution. Lane 1: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 5 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67017, 1/5,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa

Applications for Parkin Antibody (JF82-09)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

IF-Tissue-1:50-1:200, IF-Cell-1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

Use at an assay dependent concentration

Western Blot

1:500-1:1000

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-67017 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parkin

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Gene Symbol

PRKN

Additional Parkin Products

Product Documents for Parkin Antibody (JF82-09)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Parkin Antibody (JF82-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Parkin Antibody (JF82-09)

Customer Reviews for Parkin Antibody (JF82-09) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
0%
3 Stars
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2 Stars
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1 Stars
33%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Parkin Antibody (JF82-09)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: SH-SY5Y cells
    Species: Human
    Verified Customer | Posted 02/10/2022
    SH-SY5Y cells
    Parkin Antibody (JF82-09) NBP2-67017
  • Parkin Antibody (JF82-09)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: MDA MB 231 cells
    Species: Human
    Verified Customer | Posted 02/21/2019
    Parkin Antibody (JF82-09) NBP2-67017
  • Parkin Antibody (JF82-09)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse vascular smooth muscle cell
    Species: Mouse
    Verified Customer | Posted 12/21/2018
    Parkin Antibody (JF82-09) NBP2-67017

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Protocols

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