PDK-1 Antibody (24GB5425)

Name: PDK-1 Antibody (24GB5425)
Brand: Novus Biologicals
SKU: NBP3-51712
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-51712

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5425

View all PDK-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PDK-1 (Uniprot: O15530)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDK-1 Antibody (24GB5425)

Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (24GB5425)[NBP3-51712] -

Immunocytochemical staining of HepG2 cells with PDK-1 antibody (1:1,000). Nuclei were stained blue with DAPI; PDK-1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.

Flow Cytometry: PDK-1 Antibody (24GB5425)[NBP3-51712] -

Validation of PDK-1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PDK-1 antibody (1:2,000) and analyzed using BD flow cytometer.

Flow Cytometry: PDK-1 Antibody (24GB5425)[NBP3-51712] -

Flow cytometric analysis of PDK-1expression in HepG2 cells using PDK-1 antibody (1:2,000). Green, isotype control; red, PDPK1.

Western Blot: PDK-1 Antibody (24GB5425)[NBP3-51712] -

Western blotting analysis using PDK-1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Western Blot: PDK-1 Antibody (24GB5425)[NBP3-51712] -

Western blotting analysis using PDK-1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit (3-phosphoinositide dependent protein kinase 1.

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Applications for PDK-1 Antibody (24GB5425)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDK-1

PDK-1 (3-phosphoinositide-dependent protein kinase, gene name PDPK1) is a 58 -68 kDa, 556 amino acid (aa) monomeric protein of the AGC serine/threonine kinase family. It is activated by phosphorylation in the presence of PtdIns(3,4,5) P3 or PtdIns(3,4) P2. Akt, S6 kinases, PKA and PKC-zeta are reported PDK-1 substrates. Through Akt, PDK-1 mediates many of the intracellular actions of insulin. Within the region used as an immunogen, human PDK-1 shares 98% aa identity with mouse and rat PDK-1. One reported isoform has an alternate start site at aa 50, while another lacking aa 238-263 is predicted to be catalytically inactive.

Long Name

Phosphoinositide Dependent Kinase-1

Alternate Names

PDK1, PDPK1, PKB kinase

Gene Symbol

PDPK1

Additional PDK-1 Products

Product Documents for PDK-1 Antibody (24GB5425)

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Certificate of Analysis

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Product Specific Notices for PDK-1 Antibody (24GB5425)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.