PPP2R4 Antibody (4D9) - BSA Free

Novus Biologicals | Catalog # NBP2-37530

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D9

Format

BSA Free
View all PPP2R4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PPP2R4 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PPP2R4 Antibody (4D9) - BSA Free (NBP2-37530) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PPP2R4 Antibody (4D9) - BSA Free

Western Blot: PPP2R4 Antibody (4D9)BSA Free [NBP2-37530]

Western Blot: PPP2R4 Antibody (4D9)BSA Free [NBP2-37530]

Western Blot: PPP2R4 Antibody (4D9) [NBP2-37530] - Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) recombinant protein. (Expected MW is 41 kDa)
Immunocytochemistry/ Immunofluorescence: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunocytochemistry/ Immunofluorescence: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunocytochemistry/Immunofluorescence: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunohistochemistry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunohistochemistry: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining.
Flow Cytometry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Flow Cytometry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Flow Cytometry: PPP2R4 Antibody (4D9) [NBP2-37530] - Flow cytometric analysis of MCF-7 cells using PPP2R4 mouse mAb (green) and negative control (red).
Immunohistochemistry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunohistochemistry: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

Immunohistochemistry: PPP2R4 Antibody (4D9) [NBP2-37530] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PPP2R4 mouse mAb with DAB staining.
ELISA: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

ELISA: PPP2R4 Antibody (4D9) - BSA Free [NBP2-37530]

ELISA: PPP2R4 Antibody (4D9) [NBP2-37530] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PPP2R4 Antibody (4D9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPP2R4

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Alternate Names

PTPA

Gene Symbol

PTPA

Additional PPP2R4 Products

Product Documents for PPP2R4 Antibody (4D9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PPP2R4 Antibody (4D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

Customer Reviews for PPP2R4 Antibody (4D9) - BSA Free

There are currently no reviews for this product. Be the first to review PPP2R4 Antibody (4D9) - BSA Free and earn rewards!

Have you used PPP2R4 Antibody (4D9) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies