PTK9 Antibody (7C11) - BSA Free

Novus Biologicals | Catalog # NBP2-37456

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7C11

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human PTK9 (AA: 335-384) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTK9 Antibody (7C11) - BSA Free

Western Blot: PTK9 Antibody (7C11)BSA Free [NBP2-37456]

Western Blot: PTK9 Antibody (7C11)BSA Free [NBP2-37456]

Western Blot: PTK9 Antibody (7C11) [NBP2-37456] - Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunocytochemistry/ Immunofluorescence: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunocytochemistry/Immunofluorescence: PTK9 Antibody (7C11) [NBP2-37456] - Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunohistochemistry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunohistochemistry: PTK9 Antibody (7C11) [NBP2-37456] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
Flow Cytometry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Flow Cytometry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Flow Cytometry: PTK9 Antibody (7C11) [NBP2-37456] - Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).
Western Blot: PTK9 Antibody (7C11)BSA Free [NBP2-37456]

Western Blot: PTK9 Antibody (7C11)BSA Free [NBP2-37456]

Western Blot: PTK9 Antibody (7C11) [NBP2-37456] - Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa)
Immunohistochemistry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunohistochemistry: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

Immunohistochemistry: PTK9 Antibody (7C11) [NBP2-37456] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
ELISA: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

ELISA: PTK9 Antibody (7C11) - BSA Free [NBP2-37456]

ELISA: PTK9 Antibody (7C11) [NBP2-37456] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PTK9 Antibody (7C11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTK9

PTK9 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Alternate Names

A6, A6 protein tyrosine kinase, Protein A6, Protein tyrosine kinase 9MGC23788, PTK9 protein tyrosine kinase 9, PTK9MGC41876, twinfilin, actin-binding protein, homolog 1 (Drosophila), twinfilin-1

Gene Symbol

TWF1

Additional PTK9 Products

Product Documents for PTK9 Antibody (7C11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PTK9 Antibody (7C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PTK9 Antibody (7C11) - BSA Free

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Protocols

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FAQs

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