RBPMS Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-20112

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Predicted:

Bovine (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Immunofluorescence, IF/ICC, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RBPMS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBPMS Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112]

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112]

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Mouse intestine. RBPMS stained by RBPMS antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
RBPMS Antibody

Western Blot: RBPMS Antibody [NBP2-20112] -

Western Blot: RBPMS Antibody [NBP2-20112] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RBPMS Antibody

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] -

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RBPMS Antibody

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] -

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RBPMS Antibody

Immunocytochemistry/ Immunofluorescence: RBPMS Antibody [NBP2-20112] -

Immunocytochemistry/ Immunofluorescence: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
RBPMS Antibody - BSA Free

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] -

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RBPMS Antibody - BSA Free

Western Blot: RBPMS Antibody [NBP2-20112] -

Non-transfected (-) and transfected (+) HCT-116 whole cell extract were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RBPMS Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RBPMS Antibody [NBP2-20112] -

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
RBPMS Antibody - BSA Free

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] -

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: RBPMS protein stained by RBPMS antibody (NBP2-20112) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RBPMS Antibody - BSA Free

Western Blot: RBPMS Antibody [NBP2-20112] -

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RBPMS Antibody - BSA Free

Western Blot: RBPMS Antibody [NBP2-20112] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RBPMS Antibody - BSA Free

Western Blot: RBPMS Antibody [NBP2-20112] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RBPMS Antibody - BSA Free

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] -

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RBPMS Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RBPMS Antibody - Azide and BSA Free [NBP2-20112] -

Nicotinamide provides a robust, long-term retinal ganglion cell neuroprotection following intravitreal MPTP administration. A Retinal ganglion cells were labelled in whole-mounted retina with anti-RBPMS (magenta) in Thy1-CFP mice. B There was no significant difference in RBPMS+ retinal ganglion cell density between the vehicle control group and the groups treated with NAM prior to injection of 5 mg/mL MPTP or 50 mg/mL MPTP, supporting the protection of retinal ganglion cells by NAM. Scale bar = 20 um in A Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38773545), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RBPMS Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Validated from a verified customer review.

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-20112 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RBPMS

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

RNA binding protein, mRNA processing factor

Alternate Names

HERMES, RBPMS

Gene Symbol

RBPMS

Additional RBPMS Products

Product Documents for RBPMS Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RBPMS Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RBPMS Antibody - Azide and BSA Free

Customer Reviews for RBPMS Antibody - Azide and BSA Free (1)

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Customer Images

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  • RBPMS Antibody
    Name: Patricia Semedo-Kuriki
    Application: Flow Cytometry
    Sample Tested: mouse retina
    Species: Mouse
    Verified Customer | Posted 04/09/2021
    RBPMS FACS assay of retina cells. A.;Gate strategy and B.; duplets exclusion.C.; FITC fluorescence of unstained cells. D – F 1; FITC fluorescence of RBPMS of cells stained at 1:100, 1:250 and 1:500 respectively.G.; % of RBPMS positive cells and MFI.
    RBPMS Antibody - Azide and BSA Free NBP2-20112

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