RGS16 Antibody (OTI4E5)

Novus Biologicals | Catalog # NBP2-01584

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E5
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Product Specifications

Immunogen

Full length human recombinant protein of human RGS16(NP_002919) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RGS16 Antibody (OTI4E5)

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

RGS16-Antibody-OTI4E5-Western-Blot-NBP2-01584-img0009.jpg
Immunocytochemistry/ Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunocytochemistry/ Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunocytochemistry/Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16.
Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.
Flow Cytometry: RGS16 Antibody (OTI4E5) [NBP2-01584]

Flow Cytometry: RGS16 Antibody (OTI4E5) [NBP2-01584]

Flow Cytometry: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of Jurkat cells, using anti-RGS16 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS16.
Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RGS16 monoclonal antibody.
Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human breast tissue using anti-RGS16 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human endometrium tissue using anti-RGS16 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]

Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human Kidney tissue using anti-RGS16 mouse monoclonal antibody.
RGS16 Antibody (OTI4E5)

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] -

Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Hepatic RGS16 reconstitution reverses Arg2-mediated improvements in peripheral fat accumulation & glucose homeostasis. a Experimental schematic used to test the role of AAV8-mediated mouse Arg2 & Ad-RGS16 overexpression in db/db mice. b Body weight & c body composition of AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). d VO2, VCO2, energy expenditure, & RER during light & dark cycles in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). e Serum insulin (left), glucose (middle), & HOMA2 IR (right) in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). f Intraperitoneal GTT & ITT (n = 5 mice per group). g Western blot analysis of total & phosphorylated AKT in liver samples from db/db AAV8-Control & AAV8-Arg2 mice (n = 4 mice per group). For box plots, the midline represents the median, boxes represent the interquartile range & whiskers show the full range of values. For bar graphs, data represent mean+s.e.m. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student’s t-test Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30962478), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RGS16 Antibody (OTI4E5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RGS16

The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq].

Alternate Names

A28-RGS14, A28-RGS14P, hRGS-r, regulator of G-protein signaling 16, regulator of G-protein signalling 16, Retinally abundant regulator of G-protein signaling, Retinal-specific RGS, RGSR, RGS-R

Entrez Gene IDs

6004 (Human)

Gene Symbol

RGS16

Additional RGS16 Products

Product Documents for RGS16 Antibody (OTI4E5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RGS16 Antibody (OTI4E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RGS16 Antibody (OTI4E5)

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Protocols

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