RPSA Antibody (RP-01) - BSA Free

Novus Biologicals | Catalog # NBP3-09109

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Bovine, Chicken, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # RP-01

Format

BSA Free
View all RPSA Primary Antibodies »

Product Specifications

Immunogen

REPRLLVVTDPRADHQP

Specificity

The mouse monoclonal antibody RP-01 recognizes ribosomal protein SA (RPSA), which is important for formation and stability of 40S ribosomal subunit, and is overexpressed in many carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for RPSA Antibody (RP-01) - BSA Free

Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RP-01) - BSA Free [NBP3-09109]

Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RP-01) - BSA Free [NBP3-09109]

Immunocytochemistry/Immunofluorescence: RPSA Antibody (RP-01) [NBP3-09109] - Staining of RPSA in human glioblastoma cells T98G using mouse monoclonal antibody RP-01. The cells were fixed with paraformaldehyde and permeabilized using Triton X-100.
RPSA Antibody (RP-01) - BSA Free Flow Cytometry: RPSA Antibody (RP-01) - BSA Free [NBP3-09109]

Flow Cytometry: RPSA Antibody (RP-01) - BSA Free [NBP3-09109]

Separation of MOLT-4 cells stained using anti-RPSA (RP-01) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from MOLT-4 unstained by primary antibody (GAM APC, black-dashed) in Analysis (intracellular staining).
RPSA Antibody (RP-01) - BSA Free

Western Blot: Mouse Monoclonal RPSA Antibody (RP-01) [NBP3-09109] -

Western Blot: Mouse Monoclonal RPSA Antibody (RP-01) [NBP3-09109] - Western blotting analysis was performed on urea buffer extracts of Ramos, Raji, HeLa, Jurkat, and Huvec cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody RP-01 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

RPSA was detected at ~37 kDa in all tested cell lines.

Applications for RPSA Antibody (RP-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

8-12 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPSA

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 37LRPNEM/1CHD4, 67 kDa laminin receptor, 67kD, ribosomal protein SA, 67LR, Colon carcinoma laminin-binding protein, LAMBR37 kDa laminin receptor, laminin binding protein, Laminin receptor 1, laminin receptor 1 (67kD, ribosomal protein SA), Laminin-binding protein precursor p40, LamR, LAMR 1, LAMR140S ribosomal protein SA, LBP, LBP/p40, LRP, LRP/LR, Multidrug resistance-associated protein MGr1-Ag, p40, ribosomal protein SA

Gene Symbol

RPSA

Additional RPSA Products

Product Documents for RPSA Antibody (RP-01) - BSA Free

Certificate of Analysis

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Product Specific Notices for RPSA Antibody (RP-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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