Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Rabbit

Cited:

Human, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Flow Cytometry Control, In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TL2.1

Format

Azide and BSA Free
View all TLR2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse TLR2 Antibody (TL2.1) - Azide and BSA Free (NB100-56722) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. Anti-TLR2 Antibody: Cited in 39 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TLR2 Antibody (TL2.1) - Azide and BSA Free

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control (Sigma, Cat. No. M5409); purple represents anti-TLR2 antibody.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Intracellular analysis of TLR2 in 10^6 PBMs using 2 ug of TLR2 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents TLR2 antibody. goat anti-mouse IgG FITC secondary antibody was used in this test.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 PBMCs (lymphocyte-gated) using 2 ugs ofMouse IgG2a Kappa, and red represents TLR2 antibody.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 ThP1 cells using 2 ug of this antibody. The closed histogram represents cells alone, green represents isotype control, red represents anti-TLR2 antibody.
Flow (Intracellular): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Intracellular): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Intracellular): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR2 antibody.
Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.
Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow (Cell Surface): TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green represents isotype control; red represents anti-TLR2 antibody.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Cell surface flow analysis of TLR2 in 10^6 PBMCs using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR2 antibody.

Applications for TLR2 Antibody (TL2.1) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (Flo et al (2000); PMID 24512642)

ELISA

reported in scientific literature (PMID 23629653)

Flow (Cell Surface)

1-2 ug/1 million cells

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000. Use reported in scientific literature (Mempel et al (2003))

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 11160251)

Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (Flo et al (2000))

In vitro assay

reported in scientific literature (PMID 24512642)

Western Blot

reported in scientific literature (PMID 28546218)
Application Notes
Flow (Cell Surface) reported in scientific literature (PMID 24836676)

Reviewed Applications

Read 2 reviews rated 4 using NB100-56726 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (TL2.1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TLR2 Antibody (TL2.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR2 Antibody (TL2.1) - Azide and BSA Free

Customer Reviews for TLR2 Antibody (TL2.1) - Azide and BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23425360
    Species: Human
    Verified Customer | Posted 12/29/2014
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 24039915
    Species: Human
    Verified Customer | Posted 12/29/2014

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Protocols

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