TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Novus Biologicals | Catalog # NBP1-97640

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # III6F

Format

BSA Free
View all TRAIL/TNFSF10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble TRAIL.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse TRAIL/TNFSF10 Antibody (III6F) - BSA Free (NBP1-97640) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and IP. Anti-TRAIL/TNFSF10 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Western Blot: TRAIL/TNFSF10 Antibody (III6F)BSA Free [NBP1-97640]

Western Blot: TRAIL/TNFSF10 Antibody (III6F)BSA Free [NBP1-97640]

Western Blot: TRAIL/TNFSF10 Antibody (III6F) [NBP1-97640] - Lane 1: Marker [kDa]. Lane 2: 100ng Recombinant Human TRAIL/TNFSF10 Protein (24kDa). Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IR dye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.

Applications for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-97640 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAIL/TNFSF10

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Gene Symbol

TNFSF10

Additional TRAIL/TNFSF10 Products

Product Documents for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Customer Reviews for TRAIL/TNFSF10 Antibody (III6F) - BSA Free (1)

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  • TRAIL/TNFSF10 Antibody (III6F)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 06/01/2018
    Lane 1: Marker [kDa]. Lane 2: 100ng Recombinant Human TRAIL/TNFSF10 Protein (24kDa).
    Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IRdye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.
    TRAIL/TNFSF10 Antibody (III6F) - BSA Free NBP1-97640

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