Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # TK8-14

Format

Azide and BSA Free
View all VAP-1/AOC3 Primary Antibodies »

Product Specifications

Immunogen

Affinity purified VAP-1/AOC3 from tonsil stroma of human origin.

Specificity

Recognises human Vascular Adhesion Protein-1 (VAP-1/AOC3), a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. Epitope recognized by TK8-18 is present both in dimeric and monomeric forms of VAP-1/AOC3. Elevated soluble VAP-1/AOC3 serum- levels have been described in several inflammatory- diseases as well as colorectal cancer. TK8-14 is a function-blocking mAb.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free (NBP2-81043) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free [NBP2-81043]

Immunocytochemistry/ Immunofluorescence: VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free [NBP2-81043]

Immunocytochemistry/Immunofluorescence: VAP-1/AOC3 Antibody (TK8-14) - Chimeric [NBP2-81043] - Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody (TK8-14). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of TK8-14 [NBP2-81043] at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81043, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Applications for VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: VAP-1/AOC3

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been described and is located approximately 9-kb downstream.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Gene Symbol

AOC3

Additional VAP-1/AOC3 Products

Product Documents for VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VAP-1/AOC3 Antibody (TK8-14) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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