Converting Fibroblasts into Cardiomyocytes (9C Cocktail)
This protocol is intended as a guide only, for full experimental details please read the reference provided.
In Brief
Cao et al. converted human fibroblasts into functional cardiomyocytes using a 9C cocktail. The 9C-treated fibroblasts were transplanted into an infarcted mouse heart and converted to cardiomyocyte-like cells.
Human foreskin fibroblasts (HFF) were treated with 9C for 6 days and CIM for 5 days then transplanted into mice with infarcted hearts. When transplanted, 9C-treated fibroblasts were efficiently converted into cardiomyocyte-like cells.
Cocktails
| 9C Medium | Cardiac Induction Medium | ||
|---|---|---|---|
| CHIR 99021 (Cat.No. 4423) | 10 μM | CHIR 99021 (Cat.No. 4423) | 12 μM |
| A 83-01 (Cat.No. 2939) | 1 μM | BMP-4 | 25 ng/ml |
| SC1 | 1 μM | Activin A | 10 ng/ml |
| OAC-2 | 5 μM | VEGF | 10 ng/ml |
| Y-27632 (Cat.No. 1254) | 10 μM | ||
| BIX 01294 (Cat.No. 3364) | 1 μM | ||
| AS 8351 | 1 μM | ||
| SU 16f (Cat.No. 3304) | 5 μM | ||
| JNJ 10198409 (Cat.No. 6976) | 0.1 μM | ||
Reference
Cao et al. (2016) Conversion of human fibroblasts into functional cardiomyocytes by small molecules. Science 352 6290. PMID: 27127239