Y-27632 dihydrochloride

GMP Version Available: TB1254-GMP
Catalog # Availability Size / Price Qty
Y-27632 dihydrochloride | CAS No. 129830-38-2 | Rho-kinase Inhibitors
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Description: Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
Alternative Names: y27632, Y-27632 2HCl, ROCK inhibitor, ROCK1 inhibitor

Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Purity: ≥98%

Product Details
Citations (247)
Supplemental Products
Reviews (13)

Biological Activity

Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Cell-permeable, orally bioavailable and active in vivo.

Y-27632 synthesized to cGMP guidelines also available.

Applications in MESP1+ Mesoderm

Application of Y-27632 (Cat. No. 1254) in MESP1+ Mesoderm

ROCK inhibition by Y-27632 (1 μM) promotes skeletal myogenic differentiation of MESP1+ mesoderm, as shown by immunostaining for Myosin Heavy Chain+ (Green) and Myogenin (Red), and counterstaining with DAPI (Cat. No. 5748; Blue).

Data courtesy of Dr Sunny Chan and Dr Michael Kyba, University of Minnesota.

Technical Data

Soluble to 100 mM in water and to 100 mM in DMSO
Desiccate at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Derivation of novel human ground state naive pluripotent stem cells.
    Gafni O, Weinberger L, Mansour AA et al.
  2. Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition.
    Wilson et al.
    Tissue.Eng.Part C.Methods, 2016;22:1085
  3. Development of bipotent cardiac/skeletal myogenic progenitors from MESP1+ mesoderm.
    Chan et al.
    Stem Cell Reports, 2016;6:26
  4. Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling.
    Ichikawa et al.
    Cryo Letters., 2011;32:516
  5. Use and properties of ROCK-specific inhibitor Y-27632.
    Narumiya et al.
    Methods Enzymol., 2000;325:273
  6. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase.
    Uehata et al.
    Nature, 1997;389:990
  7. In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection.
    Bartfeld et al.
    Gastroenterology, 2015;148:126
  8. Generation of Cerebral Organoids from Human Pluripotent Stem Cells
    Lancaster et al.
    Nat.Protoc., 2015;9:2329
  9. SnapShot: Growing Organoids from Stem Cells.
    Sato et al.
    Cell, 2015;161:1700
  10. Establishment of gastrointestinal epithelial organoids
    Mahe et al.
    Curr.Protoc. Mouse Biol., 2014;3:217
  11. Human blood vessel organoids as a model of diabetic vasculopathy.
    Wimmer et al.
    Nature, 2019;565:505
  12. Isolation and in vitro expansion of human colonic stem cells.
    Jung et al.
    Nat.Med., 2011;17:1227
  13. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases.
    Ishizaki et al.
    Mol.Pharmacol., 2000;57:976
  14. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity.
    Santos et al.
    Nat.Commun., 2019;10:4407
  15. Direct conversion of normal and Alzheimer's Disease human fibroblasts into neuronal cells by small molecules.
    Hu et al.
    Cell Stem Cell, 2015;17:204

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Citations for Y-27632 dihydrochloride

The citations listed below are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:

247 Citations: Showing 1 - 10

  1. Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use.
    Authors: Kim Et al.
    Cell Stem Cell  2021;28:343
  2. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: Piao Et al.
    Cell Stem Cell  2021;28:217
  3. Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation
    Authors: Leibel Et al.
    Curr Protoc Stem Cell Biol  2020;54:e118
  4. Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.
    Authors: Frith and Tsakiridis
    Curr Protoc Stem Cell Biol  2019;:e81
  5. Multiparametric rapid screening of neuronal process pathology for drug target identification in HSP patient-specific neurons.
    Authors: Rehbach Et al.
    Sci Rep  2019;9:9615
  6. User-defined morphogen patterning for directing human cell fate stratification.
    Authors: Regier Et al.
    Sci Rep  2019;9:6433
  7. Butyrate Produced by Commensal Bacteria Down-Regulates Indolamine 2,3-Dioxygenase 1 (IDO-1) Expression via a Dual Mechanism in Human Intestinal Epithelial Cells.
    Authors: Martin-Gallausiaux Et al.
    Front Immunol  2019;9:2838
  8. Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells.
    Authors: Delpiano Et al.
    Front Pharmacol  2019;9:1462
  9. Quantification of human neuromuscular function through optogenetics.
    Authors: Vila Et al.
    Theranostics  2019;9:1232
  10. Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.
    Authors: Schwedhelm Et al.
    Sci Rep  2019;9:12297
  11. The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest.
    Authors: Duman Et al.
    Elife  2019;8:e47566
  12. Evaluating Shigella flexneri pathogenesis in the human enteroid model.
    Authors: Ranganathan Et al.
    Infect Immun  2019;87
  13. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and MetF. cytotoxicity in breast cancer.
    Authors: Zhuang Et al.
    Cancer Metab  2019;7:1
  14. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.
    Authors: Wang Et al.
    PeerJ  2019;6:e6143
  15. Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
    Authors: Maguire Et al.
    Curr Protoc Stem Cell Biol  2019;48:e64
  16. Protective effects of oxymatrine against DSS-induced acute intestinal inflammation in mice via blocking the RhoA/ROCK signaling pathway.
    Authors: Wang Et al.
    Biosci Rep  2019;39
  17. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.
    Authors: Crowell Et al.
    Cell Rep  2019;28:1499
  18. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
    Authors: Genga Et al.
    Cell Rep  2019;27:708
  19. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.
    Authors: Mair Et al.
    Cell Rep  2019;27:599
  20. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.
    Authors: Rhee Et al.
    Cell Rep  2019;27:2212
  21. A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons.
    Authors: Meijer Et al.
    Cell Rep  2019;27:2199
  22. Entosis Controls a Developmental Cell Clearance in C. elegans.
    Authors: Lee Et al.
    Cell Rep  2019;26:3212
  23. Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery.
    Authors: Larraufie Et al.
    Cell Rep  2019;26:1399
  24. A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
    Authors: Wilke Et al.
    Cell Host Microbe  2019;26:123
  25. Single-Cell Sequencing of iPSC-Da Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
    Authors: Lang Et al.
    Cell Stem Cell  2019;24:93
  26. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells.
    Authors: Montel-Hagen Et al.
    Cell Stem Cell  2019;24:376
  27. Establishment of porcine and human expanded potential stem cells.
    Authors: Gao Et al.
    Nat Cell Biol  2019;21:687
  28. Nuclear envelope deformation controls cell cycle progression in response to mechanical force.
    Authors: Aureille Et al.
    EMBO Rep  2019;20:e48084
  29. Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.
    Authors: Nguyen Et al.
    Stem Cells Int  2019;2019:3298432
  30. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
    Authors: Chen Et al.
    Nat.Methods  2019;18:528
  31. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment.
    Authors: Georgouli Et al.
    Cell  2019;176:757
  32. Identification of Kinases Responsible for p53-Dependent Autophagy.
    Authors: Celano Et al.
    iScience  2019;15:109
  33. FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
    Authors: Hackland Et al.
    Stem Cell Reports  2019;12:920
  34. Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways.
    Authors: Yin Et al.
    Stem Cell Reports  2019;12:488
  35. A Non-canonical Pathway with Potential for Safer Modulation of Transforming Growth Factor-β1 in Steroid-Resistant Airway Diseases.
    Authors: Li Et al.
    iScience  2019;12:232
  36. Exosomes regulate neurogenesis and circuit assembly.
    Authors: Sharma Et al.
    Proc Natl Acad Sci U S A  2019;116:16086
  37. Repurposed quinacrine synergizes with CIS. reducing the effective dose required for treatment of head and neck squamous cell carcinoma.
    Authors: Bryant Et al.
    Oncotarget  2019;10:5229
  38. Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14.
    Authors: Ervin Et al.
    Stem Cell Res Ther  2019;10:43
  39. Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction.
    Authors: Park Et al.
    Nat Commun  2019;10:3123
  40. Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential.
    Authors: Vattulainen Et al.
    Stem Cell Res Ther  2019;10:236
  41. Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.
    Authors: Hladik Et al.
    Stem Cell Res Ther  2019;10:218
  42. Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory.
    Authors: Bertero Et al.
    Nat Commun  2019;10:1538
  43. TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation.
    Authors: Ng-Blichfeldt Et al.
    Am J Physiol Lung Cell Mol Physiol  2019;
  44. Rho-Kinase Inhibition Ameliorates D.nib-Induced Endothelial Dysfunction and Pulmonary Hypertension.
    Authors: Fazakas Et al.
    Front Physiol  2018;9:537
  45. Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.
    Authors: Ackermann Et al.
    Nat Commun  2018;9:5088
  46. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
    Authors: Sheng Et al.
    Nat Commun  2018;9:4047
  47. Targeting the spliceosome for cutaneous squamous cell carcinoma therapy: a role for c-MYC and wild-type p53 in determining the degree of tumour selectivity.
    Authors: Hepburn Et al.
    Oncotarget  2018;9:23029
  48. Preclinical comparison of proteasome and ubiquitin E1 enzyme inhibitors in cutaneous squamous cell carcinoma: the identification of mechanisms of differential sensitivity.
    Authors: McHugh Et al.
    Oncotarget  2018;9:20265
  49. Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids.
    Authors: Magro-Lopez Et al.
    Stem Cell Res.Ther.  2018;9:186
  50. Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
    Authors: Levardon Et al.
    Bio Protoc  2018;8
  51. Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts.
    Authors: Tidball Et al.
    Bio Protoc  2018;8
  52. Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma.
    Authors: Pitha Et al.
    Transl Vis Sci Technol  2018;7:6
  53. Rho Kinase Inhibition by AT13148 Blocks Pancreatic Ductal Adenocarcinoma Invasion and Tumor Growth.
    Authors: Rath Et al.
    Cancer Res  2018;78:3321
  54. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells.
    Authors: Ho Et al.
    Invest Ophthalmol Vis Sci  2018;59:1969
  55. Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
    Authors: Ronaldson-Bouchard Et al.
    Nature  2018;556:239
  56. α-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein.
    Authors: Wong Et al.
    Eneuro  2018;5
  57. Impaired adhesion of induced pluripotent stem cell-derived cardiac progenitor cells (iPSC-CPCs) to isolated extracellular matrix from failing hearts.
    Authors: McKown Et al.
    Heliyon  2018;4:e00870
  58. A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
    Authors: Ford Et al.
    Dev Cell  2018;47:509
  59. Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.
    Authors: McCauley Et al.
    Curr Protoc Stem Cell Biol  2018;45:e51
  60. CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.
    Authors: Gupta Et al.
    Curr Protoc Stem Cell Biol  2018;45:e50
  61. Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation.
    Authors: Ng-Blichfeldt Et al.
    EBioMedicine  2018;36:461
  62. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5.
    Authors: Schaffer Et al.
    Cell Rep  2018;25:2470
  63. Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Authors: Mehta Et al.
    Cell Rep  2018;25:1081
  64. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell.  2018;23:123
  65. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
    Authors: Lynch Et al.
    Cell Stem Cell  2018;22:653
  66. Mouse genome-wide association studies and systems genetics uncover the genetic architecture associated with hepatic pharmacokinetic and pharmacodynamic properties of a constrained ethyl antisense oligonucleotide targeting Malat1.
    Authors: Pirie Et al.
    PLoS Genet  2018;14:e1007732
  67. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses.
    Authors: Sima Et al.
    Orphanet J Rare Dis  2018;13:54
  68. Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity.
    Authors: Sharma Et al.
    Nat Protoc  2018;13:3018
  69. Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease.
    Authors: Vu Et al.
    Orphanet J Rare Dis  2018;13:152
  70. Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector.
    Authors: Sharma Et al.
    Curr Protoc Mol Biol  2018;122:e58
  71. Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors.
    Authors: Bantounas Et al.
    Stem Cell Reports  2018;10:766
  72. Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells.
    Authors: Medelnik Et al.
    Stem Cell Reports  2018;10:1751
  73. Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules.
    Authors: Asumda Et al.
    Differentiation  2018;101:16
  74. ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth.
    Authors: Rath Et al.
    EMBO Mol Med  2017;9:198
  75. Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
    Authors: Sloan Et al.
    Neuron  2017;95:779
  76. iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.
    Authors: Abud Et al.
    Neuron  2017;94:278
  77. Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors.
    Authors: Benedicto
    Nat Commun  2017;8:15374
  78. Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
    Authors: Subramanian Et al.
    Nat Commun  2017;8:14167
  79. Membrane-bound ICAM-1 contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts.
    Authors: Bonan Et al.
    Oncotarget  2017;8:1304
  80. Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells.
    Authors: Tischfield Et al.
    Stem Cell Reports  2017;8:1135
  81. Chemerin-induced arterial contraction is Gi- and calcium-dependent.
    Authors: Ferland Et al.
    Vascul Pharmacol  2017;88:30
  82. A pluripotent stem cell-based model for post-implantation human amniotic sac development.
    Authors: Shao
    Nat Commun  2017;8(1):208
  83. ASXL3 Is a Novel Pluripotency Factor in Human Respiratory Epithelial Cells and a Potential Therapeutic Target in Small Cell Lung Cancer.
    Authors: Shukla Et al.
    Cancer Res  2017;77:6267
  84. Discovery of Aromatic Carbamates that Confer Neuroprotective Activity by Enhancing Autophagy and Inducing the Anti-Apoptotic Protein B-Cell Lymphoma 2 (Bcl-2).
    Authors: Kinarivala Et al.
    J Med Chem  2017;60:9739
  85. Derivation of telencephalic oligodendrocyte progenitors from human pluripotent stem cells.
    Authors: Major Et al.
    Curr Protoc Stem Cell Biol  2017;39:1H.10.1
  86. A modular platform for differentiation of human PSCs into all major ectodermal lineages.
    Authors: Tchieu Et al.
    Cell Stem Cell.  2017;31:399
  87. Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons.
    Authors: Wang Et al.
    Genome Res  2017;27:1783
  88. Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.
    Authors: Vermilyea Et al.
    Stem Cells Dev  2017;26:1225
  89. Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
    Authors: Kotton Et al.
    Cell Stem Cell.  2017;21:472
  90. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations.
    Authors: Lee Et al.
    Cell Stem Cell  2017;21:179
  91. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling.
    Authors: McCauley Et al.
    Cell Stem Cell  2017;20:844
  92. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia.
    Authors: Bershteyn Et al.
    Cell Stem Cell  2017;20:435
  93. Entosis Is Induced by Glucose Starvation.
    Authors: Hamann Et al.
    Cell Rep  2017;20:201
  94. In Vivo Human Somitogenesis Guides Somite Development from hPSCs.
    Authors: Xi
    Cell Rep  2017;18(6):1573
  95. Excitable Dynamics and Yap-Dependent Mechanical Cues Drive the Segmentation Clock.
    Authors: Hubaud Et al.
    Cell  2017;171:668
  96. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
    Authors: Yang Et al.
    Cell  2017;169:243
  97. An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance.
    Authors: Mohapatra Et al.
    Development  2017;144:1072
  98. Mesoderm-specific Stat3 deletion affects expression of Sox9 yielding Sox9-dependent phenotypes.
    Authors: Hall
    PLoS Genet  2017;13(2):e1006610
  99. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
    Authors: Morizane and Bonventre
    Nat Protoc  2017;12:195
  100. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions.
    Authors: Bao Et al.
    Nat Protoc  2017;12:1890
  101. Neural stem cells for disease modeling of Wolman disease and evaluation of therapeutics.
    Authors: Aguisanda Et al.
    Orphanet J Rare Dis  2017;12:120
  102. The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress.
    Authors: Shi
    Mol Brain  2017;10(1):38
  103. The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ.
    Authors: Edwards Et al.
    Sci Signal  2017;10
  104. An in vitro model of murine middle ear epithelium.
    Authors: Mulay Et al.
    Dis Model Mech  2016;9:1405
  105. Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
    Authors: Jardé Et al.
    Nature Communications  2016;7:13207
  106. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
    Authors: Guye Et al.
    Sci Rep  2016;7:10243
  107. Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics.
    Authors: Kellner Et al.
    Hippocampus  2016;6:816
  108. Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluox..
    Authors: Pei Et al.
    Sci Rep  2016;6:33822
  109. DArgic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
    Authors: Borgs Et al.
    Scientific Reports  2016;6:33377
  110. Regulation of actin dynamics by WNT-5A: implications for human airway smooth muscle contraction.
    Authors: Koopmans Et al.
    Sci Rep  2016;6:30676
  111. Tumor-induced remote ECM network orientation steers angiogenesis.
    Authors: Balcioglu Et al.
    Development  2016;6:22580
  112. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A.
    Authors: Long Et al.
    Stem Cells Transl Med  2016;5:1644
  113. Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila.
    Authors: Yu and Fernandez-Gonzalez
    Elife  2016;5
  114. ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells.
    Authors: Rath Et al.
    Sci Data  2016;3:160101
  115. Elevated Glucose Levels Promote Contractile and Cytoskeletal Gene Expression in Vascular Smooth Muscle via Rho/Protein Kinase C and Actin Polymerization.
    Authors: Hien Et al.
    J Biol Chem  2016;291:3552
  116. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
    Authors: Zeltner Et al.
    Nat Med  2016;22:1421
  117. Cryopreservation of Brain Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Is Enhanced by Rho-Associated Coiled Coil-Containing Kinase Inhibition.
    Authors: Wilson Et al.
    Tissue Eng Part C Methods  2016;22:1085
  118. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell  2016;19:217
  119. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.
    Authors: Manno Et al.
    Cell  2016;167:566
  120. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
    Authors: Foulke-Abel Et al.
    Nat Commun  2016;150:638
  121. Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using humanpluripotent stem cells.
    Authors: Palpant Et al.
    Nature Protocols  2016;12:15
  122. Genomic Instability Associated with p53 Knockdown in the Generation of Huntington's Disease Human Induced Pluripotent Stem Cells.
    Authors: Tidball Et al.
    PLoS One  2016;11:e0150372
  123. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Authors: Chal Et al.
    Nat.Protoc.  2016;11:1833
  124. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
    Authors: Stebbins Et al.
    Methods  2016;101:93
  125. Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair.
    Authors: Fernandes Et al.
    Bone Res  2015;NULL
  126. A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors.
    Authors: Rempel Et al.
    Arch Toxicol  2015;89:1599
  127. Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.
    Authors: Sakaguchi Et al.
    Mol Vis  2015;6:8896
  128. A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis.
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    Stem Cell Reports  2015;6:7286
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PDX organoid culture
By Anonymous on 03/14/2021
Application: Species: Human

PDX organoid culture

ROCK Inhibitor
By Anonymous on 09/07/2020
Application: Species: Human

To generate human intestinal organoids

PMID: 31306552 Reference

By Veselina Petrova on 08/28/2020
Application: Species: Human

I used this product on freshly split and plate human iPSC cells to help cell survival and adherence. I used the product at 10 mM concentration which I diluted in 1:1000 in culture media and left the cells at 37C, 5% CO2 for 24 hours before replacing with fresh culture media.

For hPSC culture
By Anonymous on 06/30/2020
Application: Species: Human

For hPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS. Make 100µl aliquots and keep them at −20 °C for up to 6 months.

Worked great and at a great price
By Tres Davis on 05/08/2020
Application: Species: Mouse


Neural Induction Conditions
By Anonymous on 03/22/2020
Application: Species: Human

ROCK inhibitor Y-27632 (10 mM)

PMID: 30656621

Great for passaging stem cells.
By Samiha Shaikh on 01/31/2020
Application: Species: Human

Great for passaging huiPSCs with dissociation into single cells.

Great for single iPS cell survival
By Anonymous on 09/13/2019
Application: Species: Human

Used during single cell passage for CRISPR clone isolation, works well and no difference found between multiple batches. Cells show characteristic 'spiky' morphology upon addition to the medium.

Human PDAC orgnaoids
By Anonymous on 05/03/2019
Application: Species: Human

To make Human PDAC orgnaoids

For iPSC culture
By Pinyuan Tian on 08/07/2018
Application: Species: Human

For iPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS.

ROCK Inhibitor to improve cartilage integrity
By Anonymous on 03/26/2018
Application: Species: Other

I dissolved in DMSO

I prepare 20mg of the product in 5.88mL H2O and I use 1uL/mL of the stock in my cell culture after the cell splitting
By Anonymous on 02/01/2018
Application: Species: Human

I used it in two human IPS cell lines, 5uM concentration the first 24 h after the splitting. The use of this reagent improves substantially the cell survival.

ROCK Inhibitor to improve cell survival
By Hudson DE OLIVEIRA BEZERRA on 01/05/2018
Application: Species: Human

My team uses this product to improve human embryonic stem cells survival in single cell passage methods in siRNA and CRISPR experiments. In general, we use the concentration of 10 uM. The products work very well, better than others available in the market.

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