Selective p160ROCK inhibitor (K
values are 0.14, 26, 25 and > 250
M for p160ROCK, PKC, PKA and MLCK respectively). Also inhibits PRK2 (IC
= 600 nM). Smooth muscle relaxant and orally active
. Increases survival rate of human embryonic stem (hES) cells and iPSC-BMECs undergoing cryopreservation. Also optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).
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Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors.
Authors: Bantounas Et al.
Stem Cell Reports 2018;10:766
ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth.
Authors: Rath Et al.
EMBO Mol Med 2017;9:198
Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors.
Nat Commun 2017;8:15374
Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
Authors: Subramanian Et al.
Nat Commun 2017;8:14167
Membrane-bound ICAM-1 contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts.
Authors: Bonan Et al.
A pluripotent stem cell-based model for post-implantation human amniotic sac development.
Nat Commun 2017;8(1):208
A modular platform for differentiation of human PSCs into all major ectodermal lineages.
Authors: Tchieu Et al.
Cell Stem Cell. 2017;31:399
Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
Authors: Kotton Et al.
Cell Stem Cell. 2017;21:472
Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations.
Authors: Lee Et al.
Cell Stem Cell 2017;21:179
In Vivo Human Somitogenesis Guides Somite Development from hPSCs.
Cell Rep 2017;18(6):1573
Mesoderm-specific Stat3 deletion affects expression of Sox9 yielding Sox9-dependent phenotypes.
PLoS Genet 2017;13(2):e1006610
The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress.
Mol Brain 2017;10(1):38
Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
Authors: Jardé Et al.
Nature Communications 2016;7:13207
Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
Authors: Guye Et al.
Sci Rep 2016;7:10243
Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics.
Authors: Kellner Et al.
Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
Authors: Borgs Et al.
Scientific Reports 2016;6:33377
Regulation of actin dynamics by WNT-5A: implications for human airway smooth muscle contraction.
Authors: Koopmans Et al.
Sci Rep 2016;6:30676
Tumor-induced remote ECM network orientation steers angiogenesis.
Authors: Balcioglu Et al.
Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila.
Authors: Yu and Fernandez-Gonzalez
ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells.
Authors: Rath Et al.
Sci Data 2016;3:160101
Elevated Glucose Levels Promote Contractile and Cytoskeletal Gene Expression in Vascular Smooth Muscle via Rho/Protein Kinase C and Actin Polymerization.
Authors: Hien Et al.
J Biol Chem 2016;291:3552
Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
Authors: Mou Et al.
Cell: Stem Cell 2016;19:217
Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.
Authors: Manno Et al.
Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
Authors: Foulke-Abel Et al.
Nat Commun 2016;150:638
Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using humanpluripotent stem cells.
Authors: Palpant Et al.
Nature Protocols 2016;12:15
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells
Authors: Chal Et al.
Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair.
Authors: Fernandes Et al.
Bone Res 2015;NULL
A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors.
Authors: Rempel Et al.
Arch Toxicol 2015;89:1599
Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.
Authors: Sakaguchi Et al.
Mol Vis 2015;6:8896
A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis.
Authors: Abraham Et al.
Stem Cell Reports 2015;6:7286
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
Authors: Edri Et al.
Nat Commun 2015;6:6500
Substrate stress relaxation regulates cell spreading.
Authors: Chaudhuri Et al.
A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement.
Authors: Luzzani Et al.
Int J Cancer 2015;6:6
Authors: Arbab Et al.
Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs.
Authors: Lee Et al.
Sci Rep 2015;5:11817
Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices.
Authors: Gjorevski Et al.
Sci Rep 2015;5:11458
A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics.
Authors: Romero Et al.
Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin.
Authors: Höfner Et al.
PLoS One 2015;4:503
Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies.
Authors: Lachmann Et al.
Nat Commun 2015;4:282
Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation.
Authors: Maharam Et al.
Stem Cell Res Ther 2015;3:15015
Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
Authors: Morizane Et al.
Nat Commun 2015;33:1193
Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling.
Authors: Lee Et al.
Toxicol Sci 2015;290:14740
Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632.
Authors: Su Et al.
PLoS Comput Biol 2015;21:633
RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork.
Authors: Pattabiraman Et al.
Transgenic plants as low-cost platform for chemotherapeutic drugs screening.
Authors: Vergara Et al.
J Biol Chem 2015;16:2174
Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation.
Authors: Piao Et al.
Cell Stem Cell 2015;16:198
Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis.
Authors: Madsen Et al.
EMBO Rep 2015;16:1394
Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
Authors: Ruprecht Et al.
Int J Mol Sci 2015;160:673
Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.
Authors: Jönsson Et al.
Int J Nanomedicine 2015;142:3166
Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells.
Authors: Tyson Et al.
Stem Cells 2015;142:1267
Early maturation and distinct tau pathology in induced pluripotent stem cell-derived neurons from patients with MAPT mutations.
Authors: Iovino Et al.
SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.
Authors: Choi Et al.
Stem Cell Reports 2015;136:E219
The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions.
Authors: Balcioglu Et al.
Nat Biotechnol 2015;128:1316
Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure-Function Dynamics Coupled to Cortical Development.
Authors: Ziv Et al.
Stem Cell Reports 2015;11:e1004453
Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States.
Authors: Chen Et al.
PLoS One 2015;10:e0142554
The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures.
Authors: Davies Et al.
PLoS One 2015;10:e0138531
Simvastatin Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition.
Authors: Serra Et al.
PLoS One 2015;10:e0133875
Optimization of Invasion-Specific Effects of Betulin Derivatives on Prostate Cancer Cells through Lead Development.
Authors: Härmä Et al.
Stem Cell Reports 2015;10:e0126111
Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation.
Authors: Tai Et al.
Nat Commun 2015;10:5881
Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.
Authors: Wile Et al.
Nat Protoc 2014;9:2411
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
Authors: Moon Et al.
Mucosal Immunol 2014;7:818
TRPA1 is essential for the vascular response to environmental cold exposure.
Authors: Aubdool Et al.
Korean J Physiol Pharmacol 2014;5:5732
Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
Authors: Orgaz Et al.
Nat Commun 2014;5:4255
Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain.
Authors: Mich Et al.
Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells.
Authors: Shelton Et al.
Stem Cell Reports 2014;3:516
The atypical Rho GTPase, RhoU, regulates cell-adhesion molecules during cardiac morphogenesis.
Authors: Dickover Et al.
Dev Biol 2014;389:182
Age-dependent contribution of Rho kinase in carbachol-induced contraction of human detrusor smooth muscle in vitro.
Authors: Kirschstein Et al.
Acta Pharmacol Sin 2014;35:74
Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
Authors: Huang Et al.
Nat Biotechnol 2014;32:84
Cryopreservation of human pluripotent stem cells in defined medium.
Authors: Liu and Chen
Curr Protoc Stem Cell Biol 2014;31:1C.17.1
Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease.
Authors: Luo Et al.
Curr Protoc Stem Cell Biol 2014;29:5A.7.1
Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption.
Authors: Pavlov Et al.
J Biol Chem 2014;289:28651
GABAergic terminals are a source of galanin to modulate cholinergic neuron development in the neonatal forebrain.
Authors: Keimpema Et al.
Cereb Cortex 2014;24:3277
Competition between human cells by entosis.
Authors: Sun Et al.
Cell Res 2014;24:1299
Induction of entosis by epithelial cadherin expression.
Authors: Sun Et al.
Cell Res 2014;24:1288
Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton.
Authors: McNary Et al.
Tissue Eng Part A 2014;20:921
An in vivo role for Rho kinase activation in the tumour vascular disrupting activity of combretastatin A-4 3-O-phosphate.
Authors: Williams Et al.
Br J Pharmacol 2014;171:4902
A high-throughput screen for teratogens using human pluripotent stem cells.
Authors: Kameoka Et al.
J Biomol Screen 2014;137:76
Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins.
Authors: Wrighton Et al.
Circ Res 2014;111:18126
The role of mechanical force and ROS in integrin-dependent signals.
Authors: Zeller Et al.
PLoS One 2013;8:e64897
Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation.
Authors: Barthwal Et al.
PLoS One 2013;8:e58054
Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.
Authors: Matsumoto Et al.
Orphanet J Rare Dis 2013;8:190
Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions.
Authors: Lian Et al.
Nat Protoc 2013;8:162
Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
Authors: Kirkeby Et al.
Front Cell Neurosci 2013;6:64
Activation of β 1 but not β 3 integrin increases cell traction forces.
Authors: Lin Et al.
J Neurosci 2013;587:763
Generation of integration-free and region-specific neural progenitors from primate fibroblasts.
Authors: Lu Et al.
Curr Biol 2013;3:1580
PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively.
Authors: Zhu and Nelson
Calcium release-dependent actin flow in the leading process mediates axophilic migration.
Authors: Hutchins Et al.
J Cell Biochem 2013;33:11361
mTOR regulates phagosome and entotic vacuole fission.
Authors: Krajcovic Et al.
Mol Biol Cell 2013;24:3736
Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.
Authors: Pol Et al.
Stem Cell Res 2013;247:694
A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation.
Authors: Lazzeroni Et al.
Exp Neurol 2013;18:984
Inhibition of Rho-kinase improves erectile function, increases nitric oxide signaling and decreases penile apoptosis in a rat model of cavernous nerve injury.
Authors: Hannan Et al.
Am J Physiol Lung Cell Mol Physiol 2013;189:1155
Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts.
Authors: Calvo Et al.
Nat Cell Biol 2013;15:637
Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling.
Authors: Crompton Et al.
Nat Cell Biol 2013;11:1206
Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues.
Authors: Smith Et al.
Mol Cancer Res 2013;11:1159
Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
Authors: Li Et al.
Proc Natl Acad Sci U S A 2013;110:E1452
ZFX controls the self-renewal of human embryonic stem cells.
Authors: Harel Et al.
PLoS One 2012;7:e42302
Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
Authors: Beers Et al.
Nat Protoc 2012;7:2029
DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction.
Authors: Neely Et al.
Proc Natl Acad Sci U S A 2012;3:482
Soluble epoxide hydrolase: sex differences and role in endothelial cell survival.
Authors: Gupta Et al.
Arterioscler Thromb Vasc Biol 2012;32:1936
Intra-axonal translation of RhoA promotes axon growth inhibition by CSPG.
Authors: Walker Et al.
J Cell Sci 2012;32:14442
Pregnancy reduces RhoA/Rho kinase and protein kinase C signaling pathways downstream of thromboxane receptor activation in the rat uterine artery.
Authors: Goulopoulou Et al.
Am J Physiol Heart Circ Physiol 2012;302:H2477
Neuritogenesis: the prion protein controls β1 integrin signaling activity.
Authors: Loubet Et al.
FASEB J 2012;26:678
A temporal chromatin signature in human embryonic stem cells identifies regulators of cardiac development.
Authors: Paige Et al.
Slug and Sox9 cooperatively determine the mammary stem cell state.
Authors: Guo Et al.
J Cell Sci 2012;148:1015
Synthetic lethal screening with small-molecule inhibitors provides a pathway to rational combination therapies for melanoma.
Authors: Roller Et al.
Mol Cancer Ther 2012;11:2505
Measurement and analysis of traction force dynamics in response to vasoactive agonists.
Authors: Yang Et al.
Integr Biol (Camb) 2011;3:663
RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.
Authors: Kaur Et al.
Arterioscler Thromb Vasc Biol 2011;31:657
Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling.
Authors: Abreu-Blanco Et al.
J Cell Biol 2011;193:455
The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder.
Authors: Lee Et al.
Dev Biol 2011;15:143
Wnt signaling specifies and patterns intestinal endoderm.
Authors: Sherwood Et al.
Mech Dev 2011;128:387
An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers.
Authors: Vallenius Et al.
Integr Biol (Camb) 2011;124:384
Assessment of chemical-induced impairment of human neurite outgrowth by multiparametric live cell imaging in high-density cultures.
Authors: Stiegler Et al.
Toxicol Sci 2011;121:73
Statins inhibit angiotensin II/Smad pathway and related vascular fibrosis, by a TGF-β-independent process.
Authors: Díez Et al.
PLoS One 2010;5:e14145
Endogenous patterns of mechanical stress are required for branching morphogenesis.
Authors: Gjorevski and Nelson
Cortical actin dynamics facilitate early-stage centrosome separation.
Authors: Cao Et al.
J Pharmacol Exp Ther 2010;20:770
Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.
Authors: Simões Et al.
Dev Cell 2010;19:377
Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction.
Authors: Gomez Et al.
ACS Chem Neurosci 2010;110:44
PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts.
Authors: Kim Et al.
FEBS Lett 2010;107:17206
The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries.
Authors: Silva-Santos Et al.
J Biol Chem 2009;37:1716
Cell polarity triggered by cell-cell adhesion via E-cadherin.
Authors: Desai Et al.
Br J Pharmacol 2009;122:905
Localized and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility.
Authors: Giampieri Et al.
Nat Commun 2009;11:1287
Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation.
Authors: Rodríguez-Vita Et al.
PLoS One 2008;3:e3959
Emergence of patterned stem cell differentiation within multicellular structures.
Authors: Ruiz and Chen
J Cell Sci 2008;26:2921
Antiepileptic effects of two Rho-kinase inhibitors, Y-27632 and fasudil, in mice.
Authors: Inan and Büyükafşar
Am J Pathol 2008;155:44
Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy.
Authors: Bhadriraju Et al.
J Biol Chem 2007;8:43
Insulin induces airway smooth muscle contraction.
Authors: Schaafsma Et al.
Cell Rep 2007;150:136
Fn14 is upregulated in cytokine-stimulated vascular smooth muscle cells and is expressed in human carotid atherosclerotic plaques: modulation by atorvastatin.
Authors: Muñoz-García Et al.
Proc Natl Acad Sci U S A 2006;37:2044
Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
Authors: Guillemot and Citi
Mol Biol Cell 2006;17:3569
ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion
Authors: Wyckoff Et al.
Curr Biol 2006;16:1515
Temporally and spatially coordinated roles for Rho, Rac, Cdc42 and their effectors in growth cone guidance by a physiological electric field.
Authors: Rajnicek Et al.
J Cell Sci 2006;119:1723
Endothelin-1, via ETA receptor and independently of transforming growth factor-β, increases the connective tissue growth factor in vascular smooth muscle cells.
Authors: Rodríguez-Vita Et al.
Am J Physiol Cell Physiol 2005;97:125
CXCR4 chemokine receptor and integrin signaling co-operate in mediating adhesion and chemoresistance in small cell lung cancer (SCLC) cells.
Authors: Hartmann Et al.
Signal transduction underlying carbachol-induced contraction of human urinary bladder.
Authors: Schneider Et al.
J Biol Chem 2004;309:1148
Signal transduction underlying carbachol-induced contraction of rat urinary bladder. II. Protein kinases.
Authors: Fleichman Et al.
J Pharmacol Exp Ther 2004;308:54
Regulation of MDCK cell-substratum adhesion by RhoA and myosin light chain kinase after ATP depletion.
Authors: Prahalad Et al.
Am J Physiol Cell Physiol 2004;286:C693
The activity of the plexin-A1 receptor is regulated by Rac.
Authors: Turner Et al.
J Biol Chem 2004;279:33199
Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA.
Authors: Surks Et al.
J Biol Chem 2003;278:51484
Effects of rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G(1) phase cyclin-dependent kinases.
Authors: Roovers and Assoian
Mol Cell Biol 2003;23:4283
Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle.
Authors: Wibberley Et al.
Br J Pharmacol 2003;138:757
Comparison of signalling mechanisms involved in rat mesenteric microvessel contraction by noradrenaline and sphingosylphosphorylcholine.
Authors: Altmann Et al.
Br J Pharmacol 2003;138:261
Rho protein inactivation induced apoptosis of cultured human endothelial cells.
Authors: Hippenstiel Et al.
BMC Cell Biol 2002;283:L830
Stiffness changes in cultured airway smooth muscle cells.
Authors: An Et al.
Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway.
Authors: Jung Et al.
Br J Pharmacol 2002;277:48931
Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate.
Authors: Huang Et al.
Crit Care Med 2002;277:27265
Myelin-associated glycoprotein interacts with ganglioside GT1b. A mechanism for neurite outgrowth inhibition.
Authors: Vinson Et al.
J Urol 2001;276:20280
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Keep the stock frozen at -20°C. I use it freshly added to the medium when splitting the cells.
Hudson DE OLIVEIRA BEZERRA
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