Y-27632 dihydrochloride

GMP Version Available: TB1254-GMP
GMP
Catalog # Availability Size / Price Qty
1254/10
1254/50
1254/1
Y-27632 dihydrochloride | CAS No. 129830-38-2 | Rho-kinase Inhibitors
2 Images
Description: Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
Alternative Names: y27632,Y-27632 2HCl,ROCK inhibitor,ROCK1 inhibitor

Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Purity: ≥98%

Product Details
Citations (252)
Supplemental Products
Reviews (4)

Biological Activity

Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Also used in a protocol to generate brain organoids from human iPSCs. Cell-permeable, orally bioavailable and active in vivo.

Y-27632 synthesized to cGMP guidelines also available.

For more information about how Y-27632 dihydrochloride may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts.

Scientific Data

Application of Y-27632 in MESP1+ Mesoderm. ROCK inhibition by Y-27632 (1 μM) promotes skeletal myogenic differentiation of MESP1+ mesoderm, as shown by immunostaining for Myosin Heavy Chain+ (Green) and Myogenin (Red), and counterstaining with DAPI (Catalog # 5748; Blue, Tocris). Data courtesy of Dr Sunny Chan and Dr Michael Kyba, University of Minnesota.

Technical Data

M.Wt:
320.26
Formula:
C14H21N3O.2HCl
Solubility:
Soluble to 100 mM in water and to 100 mM in DMSO
Purity:
≥98%
Storage:
Desiccate at RT
CAS No:
129830-38-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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    Cell Stem Cell, 2019;24(5):821-828.e5.
  93. Species-specific developmental timing is maintained by pluripotent stem cells ex utero
    C Barry, MT Schmitz, P Jiang, MP Schwartz, BM Duffin, S Swanson, R Bacher, JM Bolin, AL Elwell, BE McIntosh, R Stewart, JA Thomson
    Dev. Biol., 2017;423(2):101-110.
  94. Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness
    H Yao, H Zhao, J Wang, GG Haddad
    Neuroscience, 2018;0(0):.
  95. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases.
    Ishizaki et al.
    Mol.Pharmacol., 2000;57:976
  96. Chronic aerobic exercise improves insulin sensitivity and modulates Nrf2 and NF?kappaB/IkappaBalpha pathways in the skeletal muscle of rats fed with a high fat diet
    Q Yu, Z Xia, EC Liong, GL Tipoe
    Mol Med Rep, 2019;20(6):4963-4972.
  97. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity.
    Santos et al.
    Nat.Commun., 2019;10:4407
  98. Direct conversion of normal and Alzheimer's Disease human fibroblasts into neuronal cells by small molecules.
    Hu et al.
    Cell Stem Cell, 2015;17:204
  99. Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells
    T Nishimura, H Xu, M Iwasaki, D Karigane, B Saavedra, Y Takahashi, FP Suchy, S Monobe, RM Martin, M Ohtaka, M Nakanishi, SR Burrows, ML Cleary, R Majeti, A Shibuya, H Nakauchi
    Gene Ther., 2020;0(0):.
  100. Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)
    J Ma, B Feng, D Kong, J He, R Guo, AE Amponsah, W Zhang, S Zhang, F Lv, Y Song, A Liu, H Cui
    Stem Cell Res, 2020;44(0):101760.
  101. Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
    A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
    Stem Cell Res, 2020;43(0):101720.
  102. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
    EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
    Cell Stem Cell, 2020;0(0):.
  103. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets
    SC Vermilyea, A Babinski, N Tran, S To, S Guthrie, JH Kluss, JK Schmidt, GJ Wiepz, MG Meyer, ME Murphy, MR Cookson, ME Emborg, TG Golos
    Sci Rep, 2020;10(1):3447.
  104. The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
    AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
    Nature Protocols, 2021;0(0):.
  105. Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment
    SJ Tekel, N Brookhouse, K Standage-B, X Wang, DA Brafman
    Nature Protocols, 2021;16(7):3596-3624.
  106. Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    AS Genshaft, CGK Ziegler, CN Tzouanas, BE Mead, AM Jaeger, AW Navia, RP King, MD Mana, S Huang, V Mitsialis, SB Snapper, ÖH Yilmaz, T Jacks, JF Van Humbec, AK Shalek
    Nature Communications, 2021;12(1):4995.
  107. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation
    J Zhou, H He, JJ Zhang, X Liu, W Yao, C Li, T Xu, SY Yin, DY Wu, CL Dou, Q Li, J Xiang, WJ Xiong, LY Wang, JM Tang, Z Xue, X Zhang, YL Miao
    Autophagy, 2022;0(0):.
  108. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica
    Y Hong, S Shan, Y Gu, H Huang, Q Zhang, Y Han, Y Dong, Z Liu, M Huang, T Ren
    Nature Communications, 2022;13(1):1309.
  109. Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions
    G Yeo, J Xiang, J Mueller, EC Luo, B Yee, D Schafer, J Schmok, F Tan, K Rothamel, R McVicar, E Kwong, B Croker, K Jones, HL Her, CY Chen, A Vu, W Jin, S Park, P Le, K Brennan, E Kofman, Y Li, A Tankka, K Dong, Y Song, A Clark, A Carlin, EV Nostrand, S Leibel
    Research square, 2022;0(0):.
  110. Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis
    F Pecori, N Kondo, C Ogura, T Miura, M Kume, Y Minamijima, K Yamamoto, S Nishihara
    Cell Reports, 2021;36(2):109361.
  111. A pan-cancer organoid platform for precision medicine
    BM Larsen, M Kannan, LF Langer, BD Leibowitz, A Bentaieb, A Cancino, I Dolgalev, BE Drummond, JR Dry, CS Ho, G Khullar, BA Krantz, B Mapes, KE McKinnon, J Metti, JF Perera, TA Rand, V Sanchez-Fr, JM Shaxted, MM Stein, MA Streit, YC Tan, Y Zhang, E Zhao, J Venkataram, MC Stumpe, JA Borgia, A Masood, DVT Catenacci, JV Mathews, DB Gursel, JJ Wei, TH Welling, DM Simeone, KP White, AA Khan, C Igartua, AA Salahudeen
    Cell Reports, 2021;36(4):109429.
  112. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use
    TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer
    Cell Stem Cell, 2021;28(2):343-355.e5.
  113. Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration
    HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland
    Frontiers in Pharmacology, 2021;12(0):679741.
  114. Fluvastatin promotes chondrogenic differentiation of adipose-derived mesenchymal stem cells by inducing bone morphogenetic protein 2
    M Kuwahara, Y Akasaki, N Goto, I Kurakazu, T Sueishi, M Toya, T Uchida, T Tsutsui, R Hirose, H Tsushima, Y Nakashima
    Bmc Pharmacology & Toxicology, 2022;23(1):61.
  115. De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids
    A Zeleniak, C Wiegand, W Liu, C McCormick, R K, A Alavi, H Guan, S Bertera, R Lakomy, A Tajima, H Cohen, S Wong, L Balikani, B Mizerak, Z Bar-Joseph, M Trucco, I Banerjee, Y Fan
    Nature methods, 2022;0(0):.
  116. Stem-Cell-Derived beta-Like Cells with a Functional PTPN2 Knockout Display Increased Immunogenicity
    TM Triolo, JQ Matuschek, R Castro-Gut, AH Shilleh, SPM Williams, MS Hansen, K McDaniel, JM Barra, A Michels, HA Russ
    Cells, 2022;11(23):.
  117. Culture impact on the transcriptomic programs of primary and iPSC-derived human alveolar type 2 cells
    KD Alysandrat, C Garcia-de-, C Yao, P Pessina, J Huang, C Villacorta, OT Hix, K Minakin, CL Burgess, P Bawa, A Murthy, B Konda, MF Beers, BR Stripp, CF Kim, DN Kotton
    JCI Insight, 2022;0(0):.

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Citations for Y-27632 dihydrochloride

The citations listed below are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:

252 Citations: Showing 1 - 10

  1. In vitro reconstitution of epigenetic reprogramming in the human germ line.
    Authors: Murase Et al.
    Nature  2024;
  2. An in vivo neuroimmune organoid model to study human microglia phenotypes.
    Authors: Schafer Et al.
    Cell  2023;186:2111
  3. An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
    Authors: Surendran Et al.
    STAR Protoc  2022;3:101803
  4. Pancreatic islet cryopreservation by vitrification achieves high viability, function, recovery and clinical scalability for transplantation.
    Authors: Zhan Et al.
    Nat.Med.  2022;28:798
  5. Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use.
    Authors: Kim Et al.
    Cell Stem Cell  2021;28:343
  6. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: Piao Et al.
    Cell Stem Cell  2021;28:217
  7. Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation
    Authors: Leibel Et al.
    Curr Protoc Stem Cell Biol  2020;54:e118
  8. Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties.
    Authors: Panciera Et al.
    Nat Mater  2020;19:797
  9. Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.
    Authors: Frith and Tsakiridis
    Curr Protoc Stem Cell Biol  2019;:e81
  10. Multiparametric rapid screening of neuronal process pathology for drug target identification in HSP patient-specific neurons.
    Authors: Rehbach Et al.
    Sci Rep  2019;9:9615
  11. User-defined morphogen patterning for directing human cell fate stratification.
    Authors: Regier Et al.
    Sci Rep  2019;9:6433
  12. Butyrate Produced by Commensal Bacteria Down-Regulates Indolamine 2,3-Dioxygenase 1 (IDO-1) Expression via a Dual Mechanism in Human Intestinal Epithelial Cells.
    Authors: Martin-Gallausiaux Et al.
    Front Immunol  2019;9:2838
  13. Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells.
    Authors: Delpiano Et al.
    Front Pharmacol  2019;9:1462
  14. Quantification of human neuromuscular function through optogenetics.
    Authors: Vila Et al.
    Theranostics  2019;9:1232
  15. Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.
    Authors: Schwedhelm Et al.
    Sci Rep  2019;9:12297
  16. The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest.
    Authors: Duman Et al.
    Elife  2019;8:e47566
  17. Evaluating Shigella flexneri pathogenesis in the human enteroid model.
    Authors: Ranganathan Et al.
    Infect Immun  2019;87
  18. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and MetF. cytotoxicity in breast cancer.
    Authors: Zhuang Et al.
    Cancer Metab  2019;7:1
  19. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.
    Authors: Wang Et al.
    PeerJ  2019;6:e6143
  20. Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
    Authors: Maguire Et al.
    Curr Protoc Stem Cell Biol  2019;48:e64
  21. Protective effects of oxymatrine against DSS-induced acute intestinal inflammation in mice via blocking the RhoA/ROCK signaling pathway.
    Authors: Wang Et al.
    Biosci Rep  2019;39
  22. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.
    Authors: Crowell Et al.
    Cell Rep  2019;28:1499
  23. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
    Authors: Genga Et al.
    Cell Rep  2019;27:708
  24. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.
    Authors: Mair Et al.
    Cell Rep  2019;27:599
  25. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.
    Authors: Rhee Et al.
    Cell Rep  2019;27:2212
  26. A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons.
    Authors: Meijer Et al.
    Cell Rep  2019;27:2199
  27. Entosis Controls a Developmental Cell Clearance in C. elegans.
    Authors: Lee Et al.
    Cell Rep  2019;26:3212
  28. Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery.
    Authors: Larraufie Et al.
    Cell Rep  2019;26:1399
  29. A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
    Authors: Wilke Et al.
    Cell Host Microbe  2019;26:123
  30. Single-Cell Sequencing of iPSC-Da Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
    Authors: Lang Et al.
    Cell Stem Cell  2019;24:93
  31. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells.
    Authors: Montel-Hagen Et al.
    Cell Stem Cell  2019;24:376
  32. Establishment of porcine and human expanded potential stem cells.
    Authors: Gao Et al.
    Nat Cell Biol  2019;21:687
  33. Nuclear envelope deformation controls cell cycle progression in response to mechanical force.
    Authors: Aureille Et al.
    EMBO Rep  2019;20:e48084
  34. Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.
    Authors: Nguyen Et al.
    Stem Cells Int  2019;2019:3298432
  35. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
    Authors: Chen Et al.
    Nat.Methods  2019;18:528
  36. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment.
    Authors: Georgouli Et al.
    Cell  2019;176:757
  37. Identification of Kinases Responsible for p53-Dependent Autophagy.
    Authors: Celano Et al.
    iScience  2019;15:109
  38. FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
    Authors: Hackland Et al.
    Stem Cell Reports  2019;12:920
  39. Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways.
    Authors: Yin Et al.
    Stem Cell Reports  2019;12:488
  40. A Non-canonical Pathway with Potential for Safer Modulation of Transforming Growth Factor-β1 in Steroid-Resistant Airway Diseases.
    Authors: Li Et al.
    iScience  2019;12:232
  41. Exosomes regulate neurogenesis and circuit assembly.
    Authors: Sharma Et al.
    Proc Natl Acad Sci U S A  2019;116:16086
  42. Repurposed quinacrine synergizes with CIS. reducing the effective dose required for treatment of head and neck squamous cell carcinoma.
    Authors: Bryant Et al.
    Oncotarget  2019;10:5229
  43. Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14.
    Authors: Ervin Et al.
    Stem Cell Res Ther  2019;10:43
  44. Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction.
    Authors: Park Et al.
    Nat Commun  2019;10:3123
  45. Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential.
    Authors: Vattulainen Et al.
    Stem Cell Res Ther  2019;10:236
  46. Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.
    Authors: Hladik Et al.
    Stem Cell Res Ther  2019;10:218
  47. Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory.
    Authors: Bertero Et al.
    Nat Commun  2019;10:1538
  48. TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation.
    Authors: Ng-Blichfeldt Et al.
    Am J Physiol Lung Cell Mol Physiol  2019;
  49. Rho-Kinase Inhibition Ameliorates D.nib-Induced Endothelial Dysfunction and Pulmonary Hypertension.
    Authors: Fazakas Et al.
    Front Physiol  2018;9:537
  50. Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.
    Authors: Ackermann Et al.
    Nat Commun  2018;9:5088
  51. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
    Authors: Sheng Et al.
    Nat Commun  2018;9:4047
  52. Targeting the spliceosome for cutaneous squamous cell carcinoma therapy: a role for c-MYC and wild-type p53 in determining the degree of tumour selectivity.
    Authors: Hepburn Et al.
    Oncotarget  2018;9:23029
  53. Preclinical comparison of proteasome and ubiquitin E1 enzyme inhibitors in cutaneous squamous cell carcinoma: the identification of mechanisms of differential sensitivity.
    Authors: McHugh Et al.
    Oncotarget  2018;9:20265
  54. Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids.
    Authors: Magro-Lopez Et al.
    Stem Cell Res.Ther.  2018;9:186
  55. Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts.
    Authors: Tidball Et al.
    Bio Protoc  2018;8
  56. Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
    Authors: Levardon Et al.
    Bio Protoc  2018;8
  57. Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma.
    Authors: Pitha Et al.
    Transl Vis Sci Technol  2018;7:6
  58. Rho Kinase Inhibition by AT13148 Blocks Pancreatic Ductal Adenocarcinoma Invasion and Tumor Growth.
    Authors: Rath Et al.
    Cancer Res  2018;78:3321
  59. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells.
    Authors: Ho Et al.
    Invest Ophthalmol Vis Sci  2018;59:1969
  60. Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
    Authors: Ronaldson-Bouchard Et al.
    Nature  2018;556:239
  61. α-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein.
    Authors: Wong Et al.
    Eneuro  2018;5
  62. Impaired adhesion of induced pluripotent stem cell-derived cardiac progenitor cells (iPSC-CPCs) to isolated extracellular matrix from failing hearts.
    Authors: McKown Et al.
    Heliyon  2018;4:e00870
  63. A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
    Authors: Ford Et al.
    Dev Cell  2018;47:509
  64. Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.
    Authors: McCauley Et al.
    Curr Protoc Stem Cell Biol  2018;45:e51
  65. CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.
    Authors: Gupta Et al.
    Curr Protoc Stem Cell Biol  2018;45:e50
  66. Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation.
    Authors: Ng-Blichfeldt Et al.
    EBioMedicine  2018;36:461
  67. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5.
    Authors: Schaffer Et al.
    Cell Rep  2018;25:2470
  68. Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Authors: Mehta Et al.
    Cell Rep  2018;25:1081
  69. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell.  2018;23:123
  70. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
    Authors: Lynch Et al.
    Cell Stem Cell  2018;22:653
  71. Mouse genome-wide association studies and systems genetics uncover the genetic architecture associated with hepatic pharmacokinetic and pharmacodynamic properties of a constrained ethyl antisense oligonucleotide targeting Malat1.
    Authors: Pirie Et al.
    PLoS Genet  2018;14:e1007732
  72. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses.
    Authors: Sima Et al.
    Orphanet J Rare Dis  2018;13:54
  73. Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity.
    Authors: Sharma Et al.
    Nat Protoc  2018;13:3018
  74. Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease.
    Authors: Vu Et al.
    Orphanet J Rare Dis  2018;13:152
  75. Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector.
    Authors: Sharma Et al.
    Curr Protoc Mol Biol  2018;122:e58
  76. Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors.
    Authors: Bantounas Et al.
    Stem Cell Reports  2018;10:766
  77. Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells.
    Authors: Medelnik Et al.
    Stem Cell Reports  2018;10:1751
  78. Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules.
    Authors: Asumda Et al.
    Differentiation  2018;101:16
  79. ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth.
    Authors: Rath Et al.
    EMBO Mol Med  2017;9:198
  80. Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
    Authors: Sloan Et al.
    Neuron  2017;95:779
  81. iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.
    Authors: Abud Et al.
    Neuron  2017;94:278
  82. Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors.
    Authors: Benedicto
    Nat Commun  2017;8:15374
  83. Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
    Authors: Subramanian Et al.
    Nat Commun  2017;8:14167
  84. Membrane-bound ICAM-1 contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts.
    Authors: Bonan Et al.
    Oncotarget  2017;8:1304
  85. Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells.
    Authors: Tischfield Et al.
    Stem Cell Reports  2017;8:1135
  86. Chemerin-induced arterial contraction is Gi- and calcium-dependent.
    Authors: Ferland Et al.
    Vascul Pharmacol  2017;88:30
  87. A pluripotent stem cell-based model for post-implantation human amniotic sac development.
    Authors: Shao
    Nat Commun  2017;8(1):208
  88. ASXL3 Is a Novel Pluripotency Factor in Human Respiratory Epithelial Cells and a Potential Therapeutic Target in Small Cell Lung Cancer.
    Authors: Shukla Et al.
    Cancer Res  2017;77:6267
  89. Discovery of Aromatic Carbamates that Confer Neuroprotective Activity by Enhancing Autophagy and Inducing the Anti-Apoptotic Protein B-Cell Lymphoma 2 (Bcl-2).
    Authors: Kinarivala Et al.
    J Med Chem  2017;60:9739
  90. Derivation of telencephalic oligodendrocyte progenitors from human pluripotent stem cells.
    Authors: Major Et al.
    Curr Protoc Stem Cell Biol  2017;39:1H.10.1
  91. A modular platform for differentiation of human PSCs into all major ectodermal lineages.
    Authors: Tchieu Et al.
    Cell Stem Cell.  2017;31:399
  92. Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons.
    Authors: Wang Et al.
    Genome Res  2017;27:1783
  93. Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.
    Authors: Vermilyea Et al.
    Stem Cells Dev  2017;26:1225
  94. Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
    Authors: Kotton Et al.
    Cell Stem Cell.  2017;21:472
  95. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations.
    Authors: Lee Et al.
    Cell Stem Cell  2017;21:179
  96. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling.
    Authors: McCauley Et al.
    Cell Stem Cell  2017;20:844
  97. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia.
    Authors: Bershteyn Et al.
    Cell Stem Cell  2017;20:435
  98. Entosis Is Induced by Glucose Starvation.
    Authors: Hamann Et al.
    Cell Rep  2017;20:201
  99. In Vivo Human Somitogenesis Guides Somite Development from hPSCs.
    Authors: Xi
    Cell Rep  2017;18(6):1573
  100. Excitable Dynamics and Yap-Dependent Mechanical Cues Drive the Segmentation Clock.
    Authors: Hubaud Et al.
    Cell  2017;171:668
  101. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
    Authors: Yang Et al.
    Cell  2017;169:243
  102. An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance.
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Great for increasing organoids viability
By MARIA PINNA on 05/17/2023
Species: Human

Supplementing the organoids culture media with Y-27632 dihydrochloride after passaging prevents cell death and increases viability.


In vitro study
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for human iPSC cells


PDX organoid culture
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By Anonymous on 05/03/2019
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