Selective p160ROCK inhibitor (K
values are 0.14, 26, 25 and > 250
M for p160ROCK, PKC, PKA and MLCK respectively). Also inhibits PRK2 (IC
= 600 nM). Smooth muscle relaxant and orally active
. Increases survival rate of human embryonic stem (hES) cells and iPSC-BMECs undergoing cryopreservation. Also optimizes naïve human pluripotent stem cell growth and viability following naïve cell derivation from primed ESCs and iPSCs using naïve human stem cell medium (NHSM).
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All Tocris products are intended for laboratory research use only.
The citations listed below are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:
116 Citations: Showing 1 - 10 Filter your results:
Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors. Authors: Benedicto Nat Commun 2017;8:15374 A modular platform for differentiation of human PSCs into all major ectodermal lineages. Authors: Tchieu Et al. Cell Stem Cell. 2017;31:399 Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells. Authors: Kotton Et al. Cell Stem Cell. 2017;21:472 In Vivo Human Somitogenesis Guides Somite Development from hPSCs. Authors: Xi Cell Rep 2017;18(6):1573 Mesoderm-specific Stat3 deletion affects expression of Sox9 yielding Sox9-dependent phenotypes. Authors: Hall PLoS Genet 2017;13(2):e1006610 The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress. Authors: Shi Mol Brain 2017;10(1):38 Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids Authors: Jardé Et al. Nature Communications 2016;7:13207 Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Authors: Guye Et al. Sci Rep 2016;7:10243 Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics. Authors: Kellner Et al. Hippocampus 2016;6:816 Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects. Authors: Borgs Et al. Scientific Reports 2016;6:33377 Tumor-induced remote ECM network orientation steers angiogenesis. Authors: Balcioglu Et al. Development 2016;6:22580 Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Authors: Mou Et al. Cell: Stem Cell 2016;19:217 Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Authors: Foulke-Abel Et al. Nat Commun 2016;150:638 Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using humanpluripotent stem cells. Authors: Palpant Et al. Nature Protocols 2016;12:15 Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Authors: Chal Et al. Nat.Protoc. 2016;11:1833 Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair. Authors: Fernandes Et al. Bone Res 2015;NULL Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue. Authors: Sakaguchi Et al. Mol Vis 2015;6:8896 A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis. Authors: Abraham Et al. Stem Cell Reports 2015;6:7286 Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Authors: Edri Et al. Nat Commun 2015;6:6500 Substrate stress relaxation regulates cell spreading. Authors: Chaudhuri Et al. Brain 2015;6:6364 A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement. Authors: Luzzani Et al. Int J Cancer 2015;6:6 Cloning-free CRISPR. Authors: Arbab Et al. Cell 2015;5:908 Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs. Authors: Lee Et al. Sci Rep 2015;5:11817 Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Authors: Gjorevski Et al. Sci Rep 2015;5:11458 A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. Authors: Romero Et al. Elife 2015;4:e07103 Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin. Authors: Höfner Et al. PLoS One 2015;4:503 Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies. Authors: Lachmann Et al. Nat Commun 2015;4:282 Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation. Authors: Maharam Et al. Stem Cell Res Ther 2015;3:15015 Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Authors: Morizane Et al. Nat Commun 2015;33:1193 Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling. Authors: Lee Et al. Toxicol Sci 2015;290:14740 Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632. Authors: Su Et al. PLoS Comput Biol 2015;21:633 RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork. Authors: Pattabiraman Et al. Autophagy 2015;185:496 Transgenic plants as low-cost platform for chemotherapeutic drugs screening. Authors: Vergara Et al. J Biol Chem 2015;16:2174 Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation. Authors: Piao Et al. Cell Stem Cell 2015;16:198 Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis. Authors: Madsen Et al. EMBO Rep 2015;16:1394 Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Authors: Ruprecht Et al. Int J Mol Sci 2015;160:673 Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor. Authors: Jönsson Et al. Int J Nanomedicine 2015;142:3166 Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells. Authors: Tyson Et al. Stem Cells 2015;142:1267 Early maturation and distinct tau pathology in induced pluripotent stem cell-derived neurons from patients with MAPT mutations. Authors: Iovino Et al. Development 2015;138:3345 SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II. Authors: Choi Et al. Stem Cell Reports 2015;136:E219 The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions. Authors: Balcioglu Et al. Nat Biotechnol 2015;128:1316 Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure-Function Dynamics Coupled to Cortical Development. Authors: Ziv Et al. Stem Cell Reports 2015;11:e1004453 Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. Authors: Chen Et al. PLoS One 2015;10:e0142554 The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures. Authors: Davies Et al. PLoS One 2015;10:e0138531 Simvastatin Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition. Authors: Serra Et al. PLoS One 2015;10:e0133875 Optimization of Invasion-Specific Effects of Betulin Derivatives on Prostate Cancer Cells through Lead Development. Authors: Härmä Et al. Stem Cell Reports 2015;10:e0126111 Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation. Authors: Tai Et al. Nat Commun 2015;10:5881 Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs. Authors: Wile Et al. Nat Protoc 2014;9:2411 Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Authors: Moon Et al. Mucosal Immunol 2014;7:818 TRPA1 is essential for the vascular response to environmental cold exposure. Authors: Aubdool Et al. Korean J Physiol Pharmacol 2014;5:5732 Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Authors: Orgaz Et al. Nat Commun 2014;5:4255 Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. Authors: Mich Et al. Elife 2014;3:e02669 Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Authors: Shelton Et al. Stem Cell Reports 2014;3:516 Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Authors: Huang Et al. Nat Biotechnol 2014;32:84 Cryopreservation of human pluripotent stem cells in defined medium. Authors: Liu and Chen Curr Protoc Stem Cell Biol 2014;31:1C.17.1 Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease. Authors: Luo Et al. Curr Protoc Stem Cell Biol 2014;29:5A.7.1 Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption. Authors: Pavlov Et al. J Biol Chem 2014;289:28651 GABAergic terminals are a source of galanin to modulate cholinergic neuron development in the neonatal forebrain. Authors: Keimpema Et al. Cereb Cortex 2014;24:3277 Competition between human cells by entosis. Authors: Sun Et al. Cell Res 2014;24:1299 Induction of entosis by epithelial cadherin expression. Authors: Sun Et al. Cell Res 2014;24:1288 Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton. Authors: McNary Et al. Tissue Eng Part A 2014;20:921 An in vivo role for Rho kinase activation in the tumour vascular disrupting activity of combretastatin A-4 3-O-phosphate. Authors: Williams Et al. Br J Pharmacol 2014;171:4902 A high-throughput screen for teratogens using human pluripotent stem cells. Authors: Kameoka Et al. J Biomol Screen 2014;137:76 Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins. Authors: Wrighton Et al. Circ Res 2014;111:18126 The role of mechanical force and ROS in integrin-dependent signals. Authors: Zeller Et al. PLoS One 2013;8:e64897 Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation. Authors: Barthwal Et al. PLoS One 2013;8:e58054 Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Authors: Kirkeby Et al. Front Cell Neurosci 2013;6:64 Activation of β 1 but not β 3 integrin increases cell traction forces. Authors: Lin Et al. J Neurosci 2013;587:763 Generation of integration-free and region-specific neural progenitors from primate fibroblasts. Authors: Lu Et al. Curr Biol 2013;3:1580 PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively. Authors: Zhu and Nelson Cell 2013;379:235 Calcium release-dependent actin flow in the leading process mediates axophilic migration. Authors: Hutchins Et al. J Cell Biochem 2013;33:11361 mTOR regulates phagosome and entotic vacuole fission. Authors: Krajcovic Et al. Mol Biol Cell 2013;24:3736 Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate. Authors: Pol Et al. Stem Cell Res 2013;247:694 A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation. Authors: Lazzeroni Et al. Exp Neurol 2013;18:984 Inhibition of Rho-kinase improves erectile function, increases nitric oxide signaling and decreases penile apoptosis in a rat model of cavernous nerve injury. Authors: Hannan Et al. Am J Physiol Lung Cell Mol Physiol 2013;189:1155 Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts. Authors: Calvo Et al. Nat Cell Biol 2013;15:637 Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling. Authors: Crompton Et al. Nat Cell Biol 2013;11:1206 Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues. Authors: Smith Et al. Mol Cancer Res 2013;11:1159 ZFX controls the self-renewal of human embryonic stem cells. Authors: Harel Et al. PLoS One 2012;7:e42302 Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Authors: Beers Et al. Nat Protoc 2012;7:2029 DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction. Authors: Neely Et al. Proc Natl Acad Sci U S A 2012;3:482 Intra-axonal translation of RhoA promotes axon growth inhibition by CSPG. Authors: Walker Et al. J Cell Sci 2012;32:14442 A temporal chromatin signature in human embryonic stem cells identifies regulators of cardiac development. Authors: Paige Et al. Cell 2012;151:221 Slug and Sox9 cooperatively determine the mammary stem cell state. Authors: Guo Et al. J Cell Sci 2012;148:1015 Synthetic lethal screening with small-molecule inhibitors provides a pathway to rational combination therapies for melanoma. Authors: Roller Et al. Mol Cancer Ther 2012;11:2505 RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers. Authors: Kaur Et al. Arterioscler Thromb Vasc Biol 2011;31:657 Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. Authors: Abreu-Blanco Et al. J Cell Biol 2011;193:455 The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder. Authors: Lee Et al. Dev Biol 2011;15:143 Wnt signaling specifies and patterns intestinal endoderm. Authors: Sherwood Et al. Mech Dev 2011;128:387 An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers. Authors: Vallenius Et al. Integr Biol (Camb) 2011;124:384 Statins inhibit angiotensin II/Smad pathway and related vascular fibrosis, by a TGF-β-independent process. Authors: Díez Et al. PLoS One 2010;5:e14145 Endogenous patterns of mechanical stress are required for branching morphogenesis. Authors: Gjorevski and Nelson Stroke 2010;2:424 Cortical actin dynamics facilitate early-stage centrosome separation. Authors: Cao Et al. J Pharmacol Exp Ther 2010;20:770 Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction. Authors: Gomez Et al. ACS Chem Neurosci 2010;110:44 PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. Authors: Kim Et al. FEBS Lett 2010;107:17206 The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries. Authors: Silva-Santos Et al. J Biol Chem 2009;37:1716 Cell polarity triggered by cell-cell adhesion via E-cadherin. Authors: Desai Et al. Br J Pharmacol 2009;122:905 Localized and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility. Authors: Giampieri Et al. Nat Commun 2009;11:1287 Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation. Authors: Rodríguez-Vita Et al. PLoS One 2008;3:e3959 Emergence of patterned stem cell differentiation within multicellular structures. Authors: Ruiz and Chen J Cell Sci 2008;26:2921 Antiepileptic effects of two Rho-kinase inhibitors, Y-27632 and fasudil, in mice. Authors: Inan and Büyükafşar Am J Pathol 2008;155:44 Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy. Authors: Bhadriraju Et al. J Biol Chem 2007;8:43 Insulin induces airway smooth muscle contraction. Authors: Schaafsma Et al. Cell Rep 2007;150:136 Fn14 is upregulated in cytokine-stimulated vascular smooth muscle cells and is expressed in human carotid atherosclerotic plaques: modulation by atorvastatin. Authors: Muñoz-García Et al. Proc Natl Acad Sci U S A 2006;37:2044 Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. Authors: Guillemot and Citi Mol Biol Cell 2006;17:3569 ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo. Authors: Wyckoff Et al. Curr Biol 2006;16:1515 Endothelin-1, via ETA receptor and independently of transforming growth factor-β, increases the connective tissue growth factor in vascular smooth muscle cells. Authors: Rodríguez-Vita Et al. Am J Physiol Cell Physiol 2005;97:125 CXCR4 chemokine receptor and integrin signaling co-operate in mediating adhesion and chemoresistance in small cell lung cancer (SCLC) cells. Authors: Hartmann Et al. Oncogene 2005;24:4462 Signal transduction underlying carbachol-induced contraction of human urinary bladder. Authors: Schneider Et al. J Biol Chem 2004;309:1148 The activity of the plexin-A1 receptor is regulated by Rac. Authors: Turner Et al. J Biol Chem 2004;279:33199 Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. Authors: Surks Et al. J Biol Chem 2003;278:51484 Rho protein inactivation induced apoptosis of cultured human endothelial cells. Authors: Hippenstiel Et al. BMC Cell Biol 2002;283:L830 Stiffness changes in cultured airway smooth muscle cells. Authors: An Et al. Gastroenterology 2002;283:C792 Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway. Authors: Jung Et al. Br J Pharmacol 2002;277:48931 Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate. Authors: Huang Et al. Crit Care Med 2002;277:27265 Myelin-associated glycoprotein interacts with ganglioside GT1b. A mechanism for neurite outgrowth inhibition. Authors: Vinson Et al. J Urol 2001;276:20280
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