A 83-01

Tocris Bioscience | Catalog # 2939

Selective inhibitor of TGF-βRI, ALK4 and ALK7
Tocris Bioscience

Key Product Details

Description

Selective inhibitor of TGF-βRI, ALK4 and ALK7
View all TGF-beta Receptor Inhibitors »

Product Description

A 83-01 is a potent inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively). A 83-01 blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. Only weakly inhibits ALK-1, -2, -3, -6 and MAPK activity. More potent than SB 431542 (Cat. No. 1614). A 83-01 inhibits differentiation of rat induced pluripotent stem cells (riPSCs) and increases clonal expansion efficiency. Helps maintain homogeneity and long-term in vitro self-renewal of human iPSCs. Combined with other reagents, A 83-01 promotes neural differentiation of hPSCs and also converts fibroblasts into cardiomyocytes (see protocol below).

A 83-01 synthesized to Ancillary Material Grade also available.

For more information about how A 83-01 may be used, see our protocol: Converting Fibroblasts into Cardiomyocytes (9C Cocktail)

Scientific Data Images for A 83-01

Adult Stem Cell-derived Liver Organoids

Application of A 83-01 in Culture and Characterization of Adult Stem Cell-derived Liver Organoids.

Adult stem cell-derived liver organoids were cultured using A 83-01, Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), Forskolin (Catalog # 1099), N-Acetylcysteine (Catalog # 7874), along with other reagents used in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes A 83-01, HEPES (Catalog # 3173), Nicotinamide (Catalog # 4106), N-Acetylcysteine (Catalog # 7874), along with other reagents. Differentiated human liver organoids were stained using a (A) Human Serum Albumin Antibody (Catalog # MAB1455; red) and a (B) Human HNF-3beta /FoxA2 Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

iPSC-derived Human Intestinal Organoid

Application of A 83-01 in Culture and Characterization of iPSC-derived Human Intestinal Organoids.

iPSC-derived human intestinal organoids were cultured using A 83-01, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Human/Mouse/Rat Vimentin Antibody (Catalog # MAB2105; green) and a Human/Mouse Desmin Antibody (Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Human/Mouse E-Cadherin Antibody (Catalog # AF748; green), a Human MUC2 Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Catalog # 5748; blue).

Adult Stem Cell-derived Human Descending Colon Organoids

Application of A 83-01 in Culture and Characterization of Adult Stem Cell-derived Human Descending Colon Organoids.

Adult stem cells isolated from human descending colon were embedded and then cultured for 30 days in intestinal organoid culture medium, which includes A 83-01, Y-27632 dihydrochloride (Catalog # 1254), SB 202190 (Catalog # 1264), N-Acetylcysteine (Catalog # 7874), Nicotinamide (Catalog # 4106), along with other reagents used in the human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Human MUC2 Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue).(B) Organoids were fixed and stained with a Human Chromogranin A Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Human/Mouse E-Cadherin Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue).

Adult Stem Cell-derived Lung Organoids

Application of A 83-01 in Culture and Characterization of Adult Stem Cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes A 83-01, N-Acetylcysteine (Catalog # 7874), Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Adult Stem Cell-derived Lung Organoids

Application of A 83-01 in Culture and Characterization of Adult Stem Cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were embedded and then cultured for 20-60 days in lung organoid expansion medium, which includes A 83-01, N-Acetylcysteine (Catalog # 7874), Y-27632 dihydrochloride (Catalog # 1254), HEPES (Catalog # 3173), SB 202190 (Catalog # 1264), Nicotinamide (Catalog # 4106), along with other reagents used in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various lung cell types. Lung organoids were stained with (A) anti-Cytokeratin 5 (KRT5) (green) and with anti-p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (B) Anti-Podoplanin (PDPN) (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and anti- p63/TP73L (Catalog # AF1916; red) to visualize basal cells, (C, D) Anti-MUC5AC (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and anti-SOX2 (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Product Specifications for A 83-01

Molecular Weight

421.52

Formula

C25H19N5S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide

CAS Number

909910-43-6

PubChem ID

16218924

InChI Key

HIJMSZGHKQPPJS-UHFFFAOYSA-N

SMILES

S=C(NC5=CC=CC=C5)N(N=C3C4=NC(C)=CC=C4)C=C3C2=CC=NC1=CC=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 21.08 50

Preparing Stock Solutions for A 83-01

The following data is based on the product molecular weight 421.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 4.74 mL 23.72 mL 47.45 mL
2.5 mM 0.95 mL 4.74 mL 9.49 mL
5 mM 0.47 mL 2.37 mL 4.74 mL
25 mM 0.09 mL 0.47 mL 0.95 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 989 publications citing the usage of this product.

Product Documents for A 83-01

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for A 83-01

For research use only

Customer Reviews for A 83-01 (12)

4.8 out of 5
12 Customer Ratings
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Customer Images

Showing  1 - 5 of 12 reviews Showing All
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  • works for colon organoids
    Name: Claudia Aiello
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Organoids
    Verified Customer | Posted 05/14/2025
    used for human colon-derived organoids and it worked very well! I use at a concentration of 500nM.
    A 83-01 2939
  • Organoid models culture media
    Name: MARIA PINNA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 09/22/2023
    A83-01 is a required supplementation for organoid establishment, growth and expansion.
  • Works perfectly
    Name: Toni Johansson
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 04/01/2021
    To grow primary basal cells
    A 83-01 2939
  • Works well with liver organoids
    Name: Gautam Kok
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Liver organoids
    Verified Customer | Posted 11/19/2020
    Used in expansion medium for culturing liver organoids
    A 83-01 2939
  • It worked good
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC
    Verified Customer | Posted 07/04/2020
    The cells were then maintained in RM 15 days with fresh media every other day
    Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.
    A 83-01 2939
  • For TGFB inhibition
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Neural Stem Cells
    Verified Customer | Posted 06/25/2020
    Cells are treated with 1 mM A83-01 to inhibit canonical TGFB signaling
    A 83-01 2939
  • Neural Induction Conditions
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: H9
    Verified Customer | Posted 03/22/2020
    2 μM A83–01
    A 83-01 2939
  • To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 1 mM A83-01)
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: ipsc
    Verified Customer | Posted 12/04/2019
    To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate, 3 mM Chir99021, 1 mM A83-01, and 10 mM Forskolin)
    A 83-01 2939
  • good for cell culture
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: ipsc
    Verified Customer | Posted 10/08/2019
    add to cell culture to do 3d culture
    A 83-01 2939
  • works great for TGFB inhibition
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 08/08/2019
    used it to grow primary basal cells
  • for growing cells
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 02/28/2019
    used it to grow human airway basal cells
  • A 83-01 Inhibit TGFb1-activated Cellular Fibrosis in Human Mϋller Cells
    Name: Ying Yu
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Mϋller cells
    Verified Customer | Posted 01/08/2018
    Human MOI-M1 Mϋller cells seeded in tissue culture (TC)-treated plastic plate (BD). Serum starvation by DMEM medium supplemented with 1% FBS for 24h. Pretreated Human Mϋller cell with A83-01 at 0, 0.25, 0.5, 1 and 2uM for 2h. Then added 0-10 ng/mL TGFb1 for 48h. Picro-Sirius red staining, elution, and OD 540nm measurement for the fibrosis

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