Chemical Name: 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide
Biological ActivityA 83-01 is a potent inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively). Blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. Only weakly inhibits ALK-1, -2, -3, -6 and MAPK activity. More potent than SB 431542 (Cat.No. 1614). Inhibits differentiation of rat induced pluripotent stem cells (riPSCs) and increases clonal expansion efficiency. Helps maintain homogeneity and long-term in vitro self-renewal of human iPSCs. Also promotes neural differentiation of hPSCs as part of a chemical cocktail.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Organoid Models of Human and Mouse Ductal Pancreatic Cancer.
Boj SF, Hwang C, Baker LA et al.
3D mouse embryonic stem cell culture for generating inner ear organoids.
Koehler KR, Hashino E.
The ALK5 inhibitor A-83-01 inhibits smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-β.
Tojo et al.
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.
Li et al.
Cell Stem Cell, 2009;4:16
Attachment to laminin-111 facilitates transforming growth factor β-induced expression of matrix metalloproteinase-2 in synovial fibroblasts.
Hoberg et al.
In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection.
Bartfeld et al.
Generation of Cerebral Organoids from Human Pluripotent Stem Cells
Lancaster et al.
Isolation and in vitro expansion of human colonic stem cells.
Jung et al.
Identification of multipotent luminal progenitor cells in human prostate organoid cultures.
Karthaus et al.
SnapShot: Growing Organoids from Stem Cells.
Sato et al.
Establishment of gastrointestinal epithelial organoids
Mahe et al.
Curr.Protoc. Mouse Biol., 2014;3:217
Chemically defined neural conversion of human pluripotent stem cells.
Chen et al.
Methods Mol.Biol., 2019;1919:59
Citations for A 83-01
The citations listed below are publications that use Tocris products. Selected citations for A 83-01 include:
77 Citations: Showing 1 - 10
A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
Authors: Chen Et al.
Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells
Authors: Stanifer Et al.
Cell Rep 2020;32
Snake venom gland organoids.
Authors: Post Et al.
Immunoevolution of mouse pancreatic organoid isografts from preinvasive to metastatic disease.
Authors: Filippini Et al.
Sci Rep 2019;9:12286
Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model.
Authors: Ranganathan Et al.
Infect Immun 2019;87
Sera Antibody Repertoire Analyses Reveal Mechanisms of Broad and Pandemic Strain Neutralizing Responses after Human Norovirus Vaccination.
Authors: Lindesmith Et al.
Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.
Authors: Crowell Et al.
Cell Rep 2019;28:1499
Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma.
Authors: Saito Et al.
Cell Rep 2019;27:1265
Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis.
Authors: Berkers Et al.
Cell Rep 2019;26:1701
Establishment and Morphological Characterization of Patient-Derived Organoids from Breast Cancer.
Authors: Mazzucchelli Et al.
Biol Proced Online 2019;21:12
Conserved regulation of RNA processing in somatic cell reprogramming.
Authors: Kanitz Et al.
BMC Genomics 2019;20:100
Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture.
Authors: Jee Et al.
Stem Cells Int 2019;2019:8472712
Folding-function relationship of the most common cystic fibrosis-causing CFTR conductance mutants.
Authors: Willigen Et al.
Life Sci Alliance 2019;2
Chemically Defined Neural Conversion of Human Pluripotent Stem Cells
Authors: Chen Et al.
Methods Mol.Biol. 2019;1919:59
Capacitation of human na�ve pluripotent stem cells for multi-lineage differentiation.
Authors: Rostovskaya Et al.
Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors.
Authors: Tanigawa Et al.
Stem Cell Reports 2019;13:322
Activating a Reserve Neural Stem Cell Population In Vitro Enables Engraftment and Multipotency after Transplantation.
Authors: Peterson Et al.
Stem Cell Reports 2019;12:680
Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming.
Authors: Muraoka Et al.
Nat Commun 2019;10:674
Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.
Authors: Wosen Et al.
Front Immunol 2019;10:1970
LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival.
Authors: Bayrer Et al.
Nat Commun 2018;9:4055
A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
Authors: Sheng Et al.
Nat Commun 2018;9:4047
Morphological alterations of cultured human colorectal matched tumour and healthy organoids.
Authors: Kashfi Et al.
Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
Authors: Levardon Et al.
Bio Protoc 2018;8
COX-2-PGE2 Signaling Impairs Intestinal Epithelial Regeneration and Associates with TNF Inhibitor Responsiveness in Ulcerative Colitis.
Authors: Li Et al.
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Authors: Yakoub and Sadek
Cell Transplant 2018;27:393
Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer.
Authors: Barros-Silva Et al.
Cell Rep 2018;25:3504
Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.
Authors: Rajamani Et al.
Cell Stem Cell 2018;22:698
Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.
Authors: Tata Et al.
Cell Stem Cell 2018;22:668
Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
Authors: Lynch Et al.
Cell Stem Cell 2018;22:653
CRISPR-based chromatin remodeling of the endogenous Oct4 or Sox2 locus enables reprogramming to pluripotency.
Authors: Liu Et al.
Cell Stem Cell. 2018;22:252
Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture.
Authors: Peng Et al.
Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts.
Authors: Yip Et al.
PLoS One 2018;13:e0199412
Colonoscopy-based colorectal cancer modeling in mice with CRISPR-Cas9 genome editing and organoid transplantation.
Authors: Roper Et al.
Nat Protoc 2018;13:217
IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma.
Authors: Biffi Et al.
Cancer Discov 2018;
NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State.
Authors: Mulas Et al.
Stem Cell Reports 2017;9:77
Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells.
Sci Rep 2017;7:41833
Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling.
Authors: Huang Et al.
Organoid culture of human prostate cancer cell lines LNCaP and C4-2B.
Authors: Ma Et al.
Am J Clin Exp Urol 2017;5:25
Establishment of mouse expanded potential stem cells.
Authors: Yang Et al.
In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis.
Authors: Roper Et al.
Nat Biotechnol 2017;35:569
Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.
Authors: Vermilyea Et al.
Stem Cells Dev 2017;26:1225
Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.
Authors: Ruetz Et al.
Cell Stem Cell 2017;21:791
Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
Authors: Roe Et al.
Epigenetic resetting of human pluripotency.
Authors: Guo Et al.
Immunopathology of childhood celiac disease-Key role of intestinal epithelial cells.
Authors: Pietz Et al.
PLoS One 2017;12:e0185025
Anti-tumor activity of SL4 against breast cancer cells: induction of G2/M arrest through modulation of the MAPK-dependent p21 signaling pathway.
Authors: Wang Et al.
Sci Rep 2016;6:36486
Angiopoietin-like 4 promotes angiogenesis in the tendon and is increased in cyclically loaded tendon fibroblasts.
Authors: Mousavizadeh Et al.
J Physiol 2016;594:2971
Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
Authors: Mou Et al.
Cell: Stem Cell 2016;19:217
Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation.
Authors: Zhang Et al.
Cell Stem Cell 2016;18(653)
Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
Authors: Foulke-Abel Et al.
Nat Protoc 2016;150:638
Inhibition of TGFβ cell signaling for limbal explant culture in serumless, defined xeno-free conditions.
Authors: Zamudio Et al.
Exp Eye Res 2016;145:48
Organoid culture systems for prostate epithelial and cancer tissue.
Authors: Drost Et al.
Proc Natl Acad Sci U S A 2016;11:347
High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.
Authors: Zhao Et al.
Nat Commun 2015;6:8243
Amnion cell mediated immune modulation following bleo. challenge: controlling the regulatory T cell response.
Authors: Tan Et al.
Stem Cell Res Ther 2015;6:8
Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids.
Authors: Nantasanti Et al.
Stem Cell Res 2015;5:895
CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.
Authors: Kido Et al.
Inflamm Bowel Dis 2015;5:508
Transcription factor binding dynamics during human ES cell differentiation.
Authors: Tsankov Et al.
Stem Cell Reports 2015;518:344
Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.
Authors: Liao Et al.
TGFβ loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion.
Authors: Bras Et al.
Nat Genet 2015;330:29
HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.
Authors: Mattis Et al.
Exp Cell Res 2015;24:3257
Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway.
Authors: Sibony Et al.
Proc Natl Acad Sci U S A 2015;21:2778
Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
Authors: Dai Et al.
Stem Cells Int 2015;2014:182737
Organoid models of human and mouse ductal pancreatic cancer.
Authors: Boj Et al.
Long-term culture of genome-stable bipotent stem cells from adult human liver.
Authors: Huch Et al.
Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.
Authors: Yang Et al.
Stem Cell Reports 2015;112:E2337
Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases.
Authors: Weeber Et al.
Hum Mol Genet 2015;112:13308
PMA induces SnoN proteolysis and CD61 expression through an autocrine mechanism.
Authors: Li Et al.
Cell Signal 2014;26:1369
β-Cell differentiation of human pancreatic duct-derived cells after in vitro expansion.
Authors: Corritore Et al.
Cell Cycle 2014;16:456
Mechanism-based corrector combination restores δF508-CFTR folding and function.
Authors: Okiyoneda Et al.
Nat Chem Biol 2013;9:444
Generation of BAC transgenic epithelial organoids.
Authors: Schwank Et al.
Cell Reprogram 2013;8:e76871
An in vitro expansion system for generation of human iPS cell-derived hepatic progenitor-like cells exhibiting a bipotent differentiation potential.
Authors: Yanagida Et al.
PLoS One 2013;8:e67541
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
Authors: O'Malley Et al.
Immunosurveillance against tetraploidization-induced colon tumorigenesis.
Authors: Boilàve Et al.
Proc Natl Acad Sci U S A 2013;12:473
Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules.
Authors: Kearns Et al.
PLoS One 2013;11:1003
Differential regulation of Smad3 and of the type II transforming growth factor-β receptor in mitosis: implications for signaling.
Authors: Hirschhorn Et al.
PLoS One 2012;7:e43459
Porcine induced pluripotent stem cells analogous to naïve and primed embryonic stem cells of the mouse.
Authors: Telugu Et al.
Int J Dev Biol 2011;54:1703
Generation of genetically modified rats from embryonic stem cells.
Authors: Kawamata and Ochiya
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To grow primary basal cells
Used in expansion medium for culturing liver organoids
Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.
Cells are treated with 1 mM A83-01 to inhibit canonical TGFB signaling
2 μM A83–01
To start reprogramming, cultures were switched to reprogramming medium (ES medium supplemented with 10 mM Parnate, 3 mM Chir99021, 1 mM A83-01, and 10 mM Forskolin)
add to cell culture to do 3d culture
used it to grow primary basal cells
used it to grow human airway basal cells
Human MOI-M1 Mϋller cells seeded in tissue culture (TC)-treated plastic plate (BD). Serum starvation by DMEM medium supplemented with 1% FBS for 24h. Pretreated Human Mϋller cell with A83-01 at 0, 0.25, 0.5, 1 and 2uM for 2h. Then added 0-10 ng/mL TGFb1 for 48h. Picro-Sirius red staining, elution, and OD 540nm measurement for the fibrosis