Catalog Number: 2939
Chemical Name: 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide
Biological Activity
Potent inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively). Blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. Only weakly inhibits ALK-1, -2, -3, -6 and MAPK activity. More potent than SB 431542 (Cat.No. 1614). Inhibits differentiation of rat induced pluripotent stem cells (riPSCs) and increases clonal expansion efficiency. Helps maintain homogeneity and long-term in vitro self-renewal of human iPSCs.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 50 mM in DMSO
  • Purity:
  • Storage:
    Store at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. The ALK5 inhibitor A-83-01 inhibits smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-β.
    Tojo et al.
    Cancer.Sci., 2005;96:791
  2. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.
    Li et al.
    Cell Stem Cell, 2009;4:16
  3. Attachment to laminin-111 facilitates transforming growth factor β-induced expression of matrix metalloproteinase-2 in synovial fibroblasts.
    Hoberg et al.
    Ann.Rheum.Dis., 2008;66:446

The citations listed below are publications that use Tocris products. Selected citations for A 83-01 include:

Showing Results 1 - 10 of 27

  1. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell
  2. Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation.
    Authors: Zhang Et al.
    Cell Stem Cell
  3. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
    Authors: Foulke-Abel Et al.
    Nat Protoc
  4. Organoid culture systems for prostate epithelial and cancer tissue.
    Authors: Drost Et al.
    Proc Natl Acad Sci U S A
  5. High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.
    Authors: Zhao Et al.
    Nat Commun
  6. Amnion cell mediated immune modulation following bleomycin challenge: controlling the regulatory T cell response.
    Authors: Tan Et al.
    Stem Cell Res Ther
  7. Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids.
    Authors: Nantasanti Et al.
    Stem Cell Res
  8. CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.
    Authors: Kido Et al.
    Inflamm Bowel Dis
  9. Transcription factor binding dynamics during human ES cell differentiation.
    Authors: Tsankov Et al.
    Stem Cell Reports
  10. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.
    Authors: Liao Et al.
  11. TGFβ loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion.
    Authors: Bras Et al.
    Nat Genet
  12. HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.
    Authors: Mattis Et al.
    Exp Cell Res
  13. Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway.
    Authors: Sibony Et al.
    Proc Natl Acad Sci U S A
  14. Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
    Authors: Dai Et al.
    Stem Cells Int
  15. Organoid models of human and mouse ductal pancreatic cancer.
    Authors: Boj Et al.
  16. Long-term culture of genome-stable bipotent stem cells from adult human liver.
    Authors: Huch Et al.
  17. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.
    Authors: Yang Et al.
    Stem Cell Reports
  18. Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases.
    Authors: Weeber Et al.
    Hum Mol Genet
  19. β-Cell differentiation of human pancreatic duct-derived cells after in vitro expansion.
    Authors: Corritore Et al.
    Cell Cycle
  20. Mechanism-based corrector combination restores δF508-CFTR folding and function.
    Authors: Okiyoneda Et al.
    Nat Chem Biol
  21. Generation of BAC transgenic epithelial organoids.
    Authors: Schwank Et al.
    Cell Reprogram
  22. An in vitro expansion system for generation of human iPS cell-derived hepatic progenitor-like cells exhibiting a bipotent differentiation potential.
    Authors: Yanagida Et al.
    PLoS One
  23. High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
    Authors: O'Malley Et al.
  24. Immunosurveillance against tetraploidization-induced colon tumorigenesis.
    Authors: Boilàve Et al.
    Proc Natl Acad Sci U S A
  25. Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules.
    Authors: Kearns Et al.
    PLoS One
  26. Porcine induced pluripotent stem cells analogous to naïve and primed embryonic stem cells of the mouse.
    Authors: Telugu Et al.
    Int J Dev Biol
  27. Generation of genetically modified rats from embryonic stem cells.
    Authors: Kawamata and Ochiya
Expand to show all
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details


Contact Information

Molarity Calculator
Calculate the mass, volume or concentration required for a solution

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)
An example of a molarity calculation using the molarity calculator:
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?

R&D Systems Guarantee

or select another batch:


Customer Information