Catalog Number: 2939
Chemical Name: 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide
Biological Activity
Potent inhibitor of TGF-β type I receptor ALK5 kinase, type I activin/nodal receptor ALK4 and type I nodal receptor ALK7 (IC50 values are 12, 45 and 7.5 nM respectively). Blocks phosphorylation of Smad2 and inhibits TGF-β-induced epithelial-to-mesenchymal transition. Only weakly inhibits ALK-1, -2, -3, -6 and MAPK activity. More potent than SB 431542 (Cat.No. 1614). Inhibits differentiation of rat induced pluripotent stem cells (riPSCs) and increases clonal expansion efficiency. Helps maintain homogeneity and long-term in vitro self-renewal of human iPSCs.
Technical Data
  • M.Wt:
    421.52
  • Formula:
    C25H19N5S
  • Solubility:
    Soluble to 50 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Store at -20°C
  • CAS No:
    909910-43-6
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. The ALK5 inhibitor A-83-01 inhibits smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-β.
    Tojo et al.
    Cancer.Sci., 2005;96:791
  2. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.
    Li et al.
    Cell Stem Cell, 2009;4:16
  3. Attachment to laminin-111 facilitates transforming growth factor β-induced expression of matrix metalloproteinase-2 in synovial fibroblasts.
    Hoberg et al.
    Ann.Rheum.Dis., 2008;66:446
Citations:

The citations listed below are publications that use Tocris products. Selected citations for A 83-01 include:

28 Citations: Showing 1 - 10
Filter your results:

  1. Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells.
    Authors: Genovese
    Sci Rep 2017;7:41833
  2. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell 2016;19:217
  3. Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation.
    Authors: Zhang Et al.
    Cell Stem Cell 2016;18(653)
  4. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
    Authors: Foulke-Abel Et al.
    Nat Protoc 2016;150:638
  5. Organoid culture systems for prostate epithelial and cancer tissue.
    Authors: Drost Et al.
    Proc Natl Acad Sci U S A 2016;11:347
  6. High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.
    Authors: Zhao Et al.
    Nat Commun 2015;6:8243
  7. Amnion cell mediated immune modulation following bleomycin challenge: controlling the regulatory T cell response.
    Authors: Tan Et al.
    Stem Cell Res Ther 2015;6:8
  8. Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids.
    Authors: Nantasanti Et al.
    Stem Cell Res 2015;5:895
  9. CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.
    Authors: Kido Et al.
    Inflamm Bowel Dis 2015;5:508
  10. Transcription factor binding dynamics during human ES cell differentiation.
    Authors: Tsankov Et al.
    Stem Cell Reports 2015;518:344
  11. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells.
    Authors: Liao Et al.
    Cell 2015;47:469
  12. TGFβ loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion.
    Authors: Bras Et al.
    Nat Genet 2015;330:29
  13. HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.
    Authors: Mattis Et al.
    Exp Cell Res 2015;24:3257
  14. Microbial Disruption of Autophagy Alters Expression of the RISC Component AGO2, a Critical Regulator of the miRNA Silencing Pathway.
    Authors: Sibony Et al.
    Proc Natl Acad Sci U S A 2015;21:2778
  15. Maintenance and neuronal differentiation of chicken induced pluripotent stem-like cells.
    Authors: Dai Et al.
    Stem Cells Int 2015;2014:182737
  16. Organoid models of human and mouse ductal pancreatic cancer.
    Authors: Boj Et al.
    Cell 2015;160:324
  17. Long-term culture of genome-stable bipotent stem cells from adult human liver.
    Authors: Huch Et al.
    Nature 2015;160:299
  18. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.
    Authors: Yang Et al.
    Stem Cell Reports 2015;112:E2337
  19. Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases.
    Authors: Weeber Et al.
    Hum Mol Genet 2015;112:13308
  20. β-Cell differentiation of human pancreatic duct-derived cells after in vitro expansion.
    Authors: Corritore Et al.
    Cell Cycle 2014;16:456
  21. Mechanism-based corrector combination restores δF508-CFTR folding and function.
    Authors: Okiyoneda Et al.
    Nat Chem Biol 2013;9:444
  22. Generation of BAC transgenic epithelial organoids.
    Authors: Schwank Et al.
    Cell Reprogram 2013;8:e76871
  23. An in vitro expansion system for generation of human iPS cell-derived hepatic progenitor-like cells exhibiting a bipotent differentiation potential.
    Authors: Yanagida Et al.
    PLoS One 2013;8:e67541
  24. High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
    Authors: O'Malley Et al.
    Nature 2013;499:88
  25. Immunosurveillance against tetraploidization-induced colon tumorigenesis.
    Authors: Boilàve Et al.
    Proc Natl Acad Sci U S A 2013;12:473
  26. Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules.
    Authors: Kearns Et al.
    PLoS One 2013;11:1003
  27. Porcine induced pluripotent stem cells analogous to naïve and primed embryonic stem cells of the mouse.
    Authors: Telugu Et al.
    Int J Dev Biol 2011;54:1703
  28. Generation of genetically modified rats from embryonic stem cells.
    Authors: Kawamata and Ochiya
    Nature 2010;107:14223
Expand to show all 28 Citations

Have you used A 83-01?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details

Note:

Contact Information

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

R&D Systems Guarantee

or select another batch: 

Feedback

Customer Information