4-Hydroxynonenal Antibody
4-Hydroxynonenal Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human 4‑Hydroxynonenal by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human 4‑Hydroxynonenal by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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4‑Hydroxynonenal in Human Prostate. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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4‑Hydroxynonenal in Human Prostate Cancer Tissue. 4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-Hydroxynonenal
4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
Product Datasheets
Citations for 4-Hydroxynonenal Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Targeting Ferroptosis as a Novel Approach to Alleviate Aortic Dissection
Authors: N Li, X Yi, Y He, B Huo, Y Chen, Z Zhang, Q Wang, Y Li, X Zhong, R Li, XH Zhu, Z Fang, X Wei, DS Jiang
Oncogene, 2022;18(10):4118-4134.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Aberrant Transferrin and Ferritin Upregulation Elicits Iron Accumulation and Oxidative Inflammaging Causing Ferroptosis and Undermines Estradiol Biosynthesis in Aging Rat Ovaries by Upregulating NF-kappab-Activated Inducible Nitric Oxide Synthase: First Demonstration of an Intricate Mechanism
Authors: SCW Sze, L Zhang, S Zhang, K Lin, TB Ng, ML Ng, KF Lee, JKW Lam, Z Zhang, KKL Yung
International Journal of Molecular Sciences, 2022;23(20):.
Species: Rat
Sample Types: Tissue Lysates
Applications: Western Blot -
Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice
Authors: P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan
BMC complementary medicine and therapies, 2022;22(1):208.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2
Authors: G Dang, T Li, D Yang, G Yang, X Du, J Yang, Y Miao, L Han, X Ma, Y Song, B Liu, X Li, X Wang, J Feng
Redox Biology, 2022;50(0):102257.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glia maturation factor-beta induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy
Authors: C Liu, W Sun, T Zhu, S Shi, J Zhang, J Wang, F Gao, Q Ou, C Jin, J Li, JY Xu, J Zhang, H Tian, GT Xu, L Lu
Redox Biology, 2022;52(0):102292.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC/IF -
Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury-Immunohistochemical and Biochemical Analyses
Authors: M Baškovi?, D Krsnik, M Himelreich, A Katuši? Bo, N Sin?i?, Z Sonicki, D Ježek
Journal of Clinical Medicine, 2022;11(5):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Sestrin proteins protect against lipotoxicity-induced oxidative stress in the liver via suppression of c-Jun N-terminal kinases: Short title: Sestrins ameliorate hepatic lipotoxicity
Authors: Z Fang, HG Kim, M Huang, K Chowdhury, MO Li, S Liangpunsa, XC Dong
Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration
Authors: HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman
Cell Death & Disease, 2021;12(12):1128.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress
Authors: J Misra, MJ Holmes, E T Mirek, M Langevin, HG Kim, KR Carlson, M Watford, XC Dong, TG Anthony, RC Wek
Nucleic Acids Research, 2021;49(10):5726-5742.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPAR&gamma binodal complementary modules
Authors: D Das, A Paul, A Lahiri, M Adak, SK Maity, A Sarkar, S Paul, P Chakrabart
Redox Biology, 2021;45(0):102043.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm
Authors: CS Bruells, P Duschner, G Marx, G Gayan-Rami, N Frank, T Breuer, O Krenkel, F Tacke, JC Mossanen
Scientific Reports, 2021;11(1):6302.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice
Authors: M Li, CM Li, ZC Ye, J Huang, Y Li, W Lai, H Peng, TQ Lou
J. Cell. Mol. Med., 2020;0(0):.
Species: Mouse
Sample Types: Tissue
Applications: IHC-P -
Effects of curcumin on oxidative stress, inflammation and apoptosis in L-arginine induced acute pancreatitis in mice
Authors: P Siriviriya, T Chingchit, N Klaikeaw, M Chayanupat, D Werawatgan
Heliyon, 2019;5(8):e02222.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Risperidone ameliorates prefrontal cortex neural atrophy and oxidative/nitrosative stress in brain and peripheral blood of rats with neonatal ventral hippocampus lesion
Authors: H Tendilla-B, S Meneses-Pr, R Antonio Vá, M Tapia-Rodr, A Judith Váz, H Coatl-Cuay, D Martín-Her, KS MacDowell, L Garcés-Ram, JC Leza, G Flores
J. Neurosci., 2019;0(0):.
Species: Mouse
Sample Types: Cell Lystates
Applications: Western Blot -
Mechanical ventilation causes diaphragm dysfunction in newborn lambs
Authors: F Liang, G Emeriaud, DE Rassier, D Shang, E Gusev, SNA Hussain, M Sage, B Crulli, E Fortin-Pel, JP Praud, BJ Petrof
Crit Care, 2019;23(1):123.
Species: Ovine
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats
Authors: JM Gemechu, A Sharma, D Yu, Y Xie, OM Merkel, A Moszczynsk
Sci Rep, 2018;8(1):1517.
Species: Rat
Sample Types: Whole Tissue
Applications: Western Blot -
Maternal Nicotine Exposure (MNE) leads to augmented expression of the anti-oxidant adipose tissue triglyceride lipase (ATGL) long-term in the white adipose of female rat offspring
Authors: NG Barra, T VanDuzer, AC Holloway, DB Hardy
Toxicol. Sci., 2018;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
The Addition of Manganese Porphyrins during Radiation Inhibits Prostate Cancer Growth and Simultaneously Protects Normal Prostate Tissue from Radiation Damage
Authors: A Chatterjee, Y Zhu, Q Tong, EA Kosmacek, EZ Lichter, RE Oberley-De
Antioxidants (Basel), 2018;7(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Tocotrienol-rich fraction supplementation reduces hyperglycemia-induced skeletal muscle damage through regulation of insulin signaling and oxidative stress in type 2 diabetic mice
Authors: H Lee, Y Lim
J. Nutr. Biochem., 2018;57(0):77-85.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration
Authors: WS Hambright, RS Fonseca, L Chen, R Na, Q Ran
Redox Biol, 2017;12(0):8-17.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Gamma-tocopherol supplementation ameliorated hyper-inflammatory response during the early cutaneous wound healing in alloxan-induced diabetic mice
Authors: J Shin, SJ Yang, Y Lim
Exp. Biol. Med. (Maywood), 2017;242(5):505-515.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot -
Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice
Authors: J Zhao, Z Tian, T Kadomatsu, P Xie, K Miyata, T Sugizaki, M Endo, S Zhu, H Fan, H Horiguchi, J Morinaga, K Terada, T Yoshizawa, K Yamagata, Y Oike
J. Biol. Chem., 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene
Authors: J Seo, NN Singh, EW Ottesen, S Sivanesan, M Shishimoro, RN Singh
PLoS ONE, 2016;11(4):e0154390.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress.
Authors: Garate I, Garcia-Bueno B, Madrigal J, Caso J, Alou L, Gomez-Lus M, Leza J
J Neuroinflammation, 2014;11(0):8.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
The asymmetric segregation of damaged proteins is stem cell-type dependent.
Authors: Bufalino, Mary Ros, DeVeale, Brian, van der Kooy, Derek
J Cell Biol, 2013;201(4):523-30.
Species: Drosophila
Sample Types: Whole Tissue
Applications: IHC -
Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels.
Authors: Margaritis M, Antonopoulos A, Digby J, Lee R, Reilly S, Coutinho P, Shirodaria C, Sayeed R, Petrou M, De Silva R, Jalilzadeh S, Demosthenous M, Bakogiannis C, Tousoulis D, Stefanadis C, Choudhury R, Casadei B, Channon K, Antoniades C
Circulation, 2013;127(22):2209-21.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress.
Diabetes, 2012;61(8):2093-104.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease.
J Neuroinflammation, 2012;9(0):241.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.
Authors: Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, Oike Y
J. Biol. Chem., 2011;286(5):3992-4002.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress.
Authors: Walson KH, Tang M, Glumac A, Alexander H, Manole MD, Ma L, Hsia CJ, Clark RS, Kochanek PM, Kagan VE, Bayr H
Crit. Care Med., 2011;39(2):335-43.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation.
Authors: Wang Z, Dou X, Gu D, Shen C, Yao T, Nguyen V, Braunschweig C, Song Z
Mol. Cell. Endocrinol., 2011;349(2):222-31.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cell sex determines anoikis resistance in vascular smooth muscle cells.
Authors: Straface E, Vona R, Gambardella L, Ascione B, Marino M, Bulzomi P, Canu S, Coinu R, Rosano G, Malorni W, Franconi F
FEBS Lett., 2009;583(21):3448-54.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis.
Authors: Margutti P, Matarrese P, Conti F, Colasanti T, Delunardo F, Capozzi A, Garofalo T, Profumo E, Rigano R, Siracusano A, Alessandri C, Salvati B, Valesini G, Malorni W, Sorice M, Ortona E
Blood, 2007;111(9):4559-70.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
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For the Western Blot data image for Catalog # MAB3249, how long were the HepG2 cells treated with 4-HNE prior to analysis?
The HepG2 cells were treated with 100 µM 4-HNE for 1 hour before lysing the cells.
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Reviews for 4-Hydroxynonenal Antibody
Average Rating: 5 (Based on 2 Reviews)
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-Fixed in 10% neutral buffered formalin followed by dehydration process
-10 um thick cryosection
- Blocking: Normal Goat Serum (NGS)
- Primary Ab (4-HNE): diluted in NGS at 20 ug/mL; 4C overnight
- Secondary (AF488): 1:100 dilution in NGS; room temp 1hr
- Vector anti-fade mounting media with DAPI
