4-Hydroxynonenal Antibody

(13 citations)   
  • Specificity
    Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 198960
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-coupled 4-Hydroxynonenal
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human 4‑Hydroxynonenal by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007).Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human 4‑Hydroxynonenal by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with
4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-Hydroxynonenal
4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
  • Alternate Names:
    4 HNE; 4Hydroxynonenal; 4-Hydroxynonenal; HNE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Ablation of ferroptosis regulator glutathione peroxidase 4 in forebrain neurons promotes cognitive impairment and neurodegeneration
    Authors: WS Hambright, RS Fonseca, L Chen, R Na, Q Ran
    Redox Biol, 2017;12(0):8-17.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene
    Authors: J Seo, NN Singh, EW Ottesen, S Sivanesan, M Shishimoro, RN Singh
    PLoS ONE, 2016;11(4):e0154390.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
    Authors: Holloway, A C, Salomon, A, Soares, M J, Garnier, V, Raha, S, Sergent, F, Nicholson, C J, Feige, J J, Benharouga, M, Alfaidy, N
    Am J Physiol Endocrinol Metab, 2014;306(4):E443-56.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  4. Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress.
    Authors: Garate I, Garcia-Bueno B, Madrigal J, Caso J, Alou L, Gomez-Lus M, Leza J
    J Neuroinflammation, 2014;11(0):8.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  5. The asymmetric segregation of damaged proteins is stem cell-type dependent.
    Authors: Bufalino, Mary Ros, DeVeale, Brian, van der Kooy, Derek
    J Cell Biol, 2013;201(4):523-30.
    Species: Drosophila
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  6. Interactions between vascular wall and perivascular adipose tissue reveal novel roles for adiponectin in the regulation of endothelial nitric oxide synthase function in human vessels.
    Authors: Margaritis M, Antonopoulos A, Digby J, Lee R, Reilly S, Coutinho P, Shirodaria C, Sayeed R, Petrou M, De Silva R, Jalilzadeh S, Demosthenous M, Bakogiannis C, Tousoulis D, Stefanadis C, Choudhury R, Casadei B, Channon K, Antoniades C
    Circulation, 2013;127(22):2209-21.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  7. Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress.
    Diabetes, 2012;61(8):2093-104.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease.
    J Neuroinflammation, 2012;9(0):241.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress.
    Authors: Walson KH, Tang M, Glumac A, Alexander H, Manole MD, Ma L, Hsia CJ, Clark RS, Kochanek PM, Kagan VE, Bayr H
    Crit. Care Med., 2011;39(2):335-43.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  10. Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.
    Authors: Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, Oike Y
    J. Biol. Chem., 2011;286(5):3992-4002.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. 4-Hydroxynonenal differentially regulates adiponectin gene expression and secretion via activating PPARgamma and accelerating ubiquitin-proteasome degradation.
    Authors: Wang Z, Dou X, Gu D, Shen C, Yao T, Nguyen V, Braunschweig C, Song Z
    Mol. Cell. Endocrinol., 2011;349(2):222-31.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  12. Cell sex determines anoikis resistance in vascular smooth muscle cells.
    Authors: Straface E, Vona R, Gambardella L, Ascione B, Marino M, Bulzomi P, Canu S, Coinu R, Rosano G, Malorni W, Franconi F
    FEBS Lett., 2009;583(21):3448-54.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  13. Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis.
    Authors: Margutti P, Matarrese P, Conti F, Colasanti T, Delunardo F, Capozzi A, Garofalo T, Profumo E, Rigano R, Siracusano A, Alessandri C, Salvati B, Valesini G, Malorni W, Sorice M, Ortona E
    Blood, 2007;111(9):4559-70.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 13 Citations
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