A-RAF Antibody (3E2) - Azide and BSA Free

Name: A-RAF Antibody (3E2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00000369-M05
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00000369-M05

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E2

Format

Azide and BSA Free

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Product Specifications

Immunogen

ARAF (NP_001645, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGS

Specificity

ARAF - v-raf murine sarcoma 3611 viral oncogene homolog

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for A-RAF Antibody (3E2) - Azide and BSA Free

Western Blot: A-RAF Antibody (3E2) [H00000369-M05]

Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2. Lane 1: ARAF transfected lysatE (67.6 KDa). Lane 2: Non-transfected lysate.

Western Blot: A-RAF Antibody (3E2) [H00000369-M05]

Western Blot: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of ARAF expression in HeLa (Cat # L013V1).

ELISA: A-RAF Antibody (3E2) [H00000369-M05]

ELISA: A-RAF Antibody (3E2) [H00000369-M05] - Detection limit for recombinant GST tagged ARAF is approximately 0.1ng/ml as a capture antibody.

Proximity Ligation Assay: A-RAF Antibody (3E2) [H00000369-M05]

Proximity Ligation Assay: A-RAF Antibody (3E2) [H00000369-M05] - Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

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Applications for A-RAF Antibody (3E2) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: A-Raf

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Long Name

v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

Alternate Names

ARaf, PKS, PKS2, RAFA1

Entrez Gene IDs

369 (Human)

Gene Symbol

ARAF

OMIM

311 (Human)

UniProt

NP_001645 (Human)

Additional A-Raf Products

Product Documents for A-RAF Antibody (3E2) - Azide and BSA Free

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Product Specific Notices for A-RAF Antibody (3E2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.