ABCA1 Antibody (1276B) - BSA Free

Novus Biologicals | Catalog # NBP2-54792

Recombinant Monoclonal Antibody.
Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1276B expressed in HEK293

Format

BSA Free
View all ABCA1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human ABCA1 Antibody (1276B) (between amino acids 1220-1280) [UniProt# O95477]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ABCA1 Antibody (1276B) - BSA Free (NBP2-54792) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-ABCA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ABCA1 Antibody (1276B) - BSA Free

Western Blot: ABCA1 Antibody (1276B)BSA Free [NBP2-54792]

Western Blot: ABCA1 Antibody (1276B)BSA Free [NBP2-54792]

Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.
Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Flow (Intracellular): ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).
Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunocytochemistry/ Immunofluorescence: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) - BSA Free [NBP2-54792]

Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.

Applications for ABCA1 Antibody (1276B) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-2.5 ug/mL

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

2 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCA1

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Long Name

ATP-binding Cassette, Sub-family A (ABC1), Member 1

Alternate Names

ABC1, CERP, HDLDT1, TGD

Gene Symbol

ABCA1

Additional ABCA1 Products

Product Documents for ABCA1 Antibody (1276B) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ABCA1 Antibody (1276B) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Eicosanoids and Regulators

Citations for ABCA1 Antibody (1276B) - BSA Free

Customer Reviews for ABCA1 Antibody (1276B) - BSA Free

There are currently no reviews for this product. Be the first to review ABCA1 Antibody (1276B) - BSA Free and earn rewards!

Have you used ABCA1 Antibody (1276B) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ABCA1 Antibody (1276B) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?

    A: For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.

  • Q: Our customer would like to consult something about the ABCA1 antibody with the Cat No. NB400-105. In the datasheet, it showed this protein expressed very low in most cell types. The customer may test the mouse liver tissues, and he doesn’t know the expression level of ABCA1 in this tissue. Is it necessary to induce the expression via LXR treatment to get enough protein expressed? If he doesn’t induce the expression, how much protein loaded was suitable as recommended?

    A: Our own Western blot data for NB400-105 was generated by treating RAW cells with 9-cis-retinoic acid and 22R-hydroxycholesterol, which are both known to induce expression of ABCA1 in macrophages. 40ug of protein was loaded on to the gel in this instance. Unfortunately we do not have any testing data from mouse liver tissues using this particular antibody, however the data for another of our ABCA1 antibodies may be of interest to your customer: NB100-2068. The Western blot data for this product was generated using mouse liver tissue, and a clear band can be seen at the expected molecular weight in wild type tissue. We loaded 30ug of protein when running this Western blot, however, your customer may need to optimise the staining procedure for their sample type.

  • Q: For Western blot protocol for ABCA1 antibody NB400-105, why does it suggest treating with beta-ME while not heating?

    A: For some membrane proteins it is not recommended to boil the samples. You may incubate them at room temperature or at 37C in SDS sample buffer with BME. However, from personal experience, if you boil the samples you will not see the protein on the blot. I do not think the exact reason is known.

  • Q: Our customer would like to consult something about the ABCA1 antibody with the Cat No. NB400-105. In the datasheet, it showed this protein expressed very low in most cell types. The customer may test the mouse liver tissues, and he doesn’t know the expression level of ABCA1 in this tissue. Is it necessary to induce the expression via LXR treatment to get enough protein expressed? If he doesn’t induce the expression, how much protein loaded was suitable as recommended?

    A: Our own Western blot data for NB400-105 was generated by treating RAW cells with 9-cis-retinoic acid and 22R-hydroxycholesterol, which are both known to induce expression of ABCA1 in macrophages. 40ug of protein was loaded on to the gel in this instance. Unfortunately we do not have any testing data from mouse liver tissues using this particular antibody, however the data for another of our ABCA1 antibodies may be of interest to your customer: NB100-2068. The Western blot data for this product was generated using mouse liver tissue, and a clear band can be seen at the expected molecular weight in wild type tissue. We loaded 30ug of protein when running this Western blot, however, your customer may need to optimise the staining procedure for their sample type.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies