Chemical Name: Artificial cerebrospinal fluid
Biological ActivityArtificial cerebrospinal fluid (aCSF) is commonly used to maintain the oxygen supply, osmolarity and buffer pH of isolated neurons and brain slices in electrophysiology experiments. ACSF closely matches the electrolyte concentrations of cerebrospinal fluid and is prepared from high purity water and analytical grade reagents. Microfiltered and sterile. Final ion concentrations (in mM): Na+ 150; K+ 3.0; Ca2+ 1.4; Mg2+ 0.8; P 1.0; Cl- 155.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
Byun et al.
PLoS One, 2012;7:e37917
Citations for ACSF
The citations listed below are publications that use Tocris products. Selected citations for ACSF include:
22 Citations: Showing 1 - 10
Anatomical basis and physiological role of cerebrospinal fluid transport through the murine cribriform plate.
Authors: Norwood Et al.
Mitochondrial Dynamin-Related Protein 1 (DRP1) translocation in response to cerebral glucose is impaired in a rat model of early alteration in hypothalamic glucose sensing.
Authors: Desmoulins Et al.
Mol Metab 2019;20:166
Tanycyte-Independent Control of Hypothalamic Leptin Signaling.
Authors: Yoo Et al.
Front Neurosci 2019;13:240
Ghrelin's effects on food motivation in rats are not limited to palatable foods.
Authors: Bake Et al.
J Neuroendocrinol 2018;:e12665
Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis.
Authors: Hill Et al.
Acute ghrelin changes food preference from a high-fat diet to chow during binge-like eating in rodents.
Authors: Bake Et al.
J Neuroendocrinol 2017;29
Mitochondrial Dynamics Mediated by Mitofusin 1 Is Required for POMC Neuron Glucose-Sensing and ins. Release Control.
Authors: Ramírez Et al.
Cell Metab 2017;25:1390
Astrocytic Process Plasticity and IKKβ/NF-κB in Central Control of Blood Glucose, Blood Pressure, and Body Weight.
Authors: Zhang Et al.
Cell Metab 2017;25:1091
SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production.
Authors: Mitroi Et al.
Angiotensin AT1A receptors on leptin receptor-expressing cells control resting metabolism.
Authors: Claflin Et al.
J Clin Invest 2017;127:1414
Absence of systemic oxidative stress and increased CSF prostaglandin F2α in progressive MS
Authors: Lam Et al.
Neurology: Neuroimmunology & Neuroinflammation 2016;3:256
The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia.
Authors: Kurland Et al.
J Neuroinflammation 2016;13:130
DNA repair factor BRCA1 depletion occurs in Alzheimer brains and impairs cognitive function in mice.
Authors: Suberbielle Et al.
Front Pharmacol 2015;6:8897
The depressor response to intracerebroventricular hypotonic saline is sensitive to TRPV4 antagonist RN1734.
Authors: Feetham Et al.
Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress.
Authors: Rau Et al.
Nat Commun 2015;35:9730
Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity.
Authors: Sartor Et al.
J Neurosci 2015;35:15062
Hypothalamic eIF2α signaling regulates food intake.
Authors: Maurin Et al.
Cell Rep 2014;6:438
Investigating local and long-range neuronal network dynamics by simultaneous optogenetics, reverse microdialysis and silicon probe recordings in vivo.
Authors: Taylor Et al.
J Neurosci 2014;235:83
Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics.
Authors: McCall Et al.
Nat Protoc 2013;8:2413
Detection of cerebral spinal fluid-associated chemokines in birth traumatized premature babies by chip-based immunoaffinity CE.
Authors: Phillips and Wellner
J Biol Chem 2013;34:1530
Central 5-α reduction of testost. is required for testosterone's inhibition of the hypothalamo-pituitary-adrenal axis response to restraint stress in adult male rats.
Authors: Handa Et al.
J Neurosci Methods 2013;1529:74
Electrode robustness in artificial cerebrospinal fluid for dielectrophoresis-based LoC.
Authors: Miled & Sawan
Conf Proc IEEE Eng Med Biol Soc 2012;2012:1390
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We use aCSF to infuse drugs into the brain. We usually dissolve the different drugs that we infuse in aCSF and then use aCSF infusions as a control
In vivo application as solvent in brain infusion experiment in mice.