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ACSF | Buffers, Chelators and Reagents
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Description: Artificial cerebrospinal fluid

Chemical Name: Artificial cerebrospinal fluid

Product Details
Citations (22)
Reviews (2)

Biological Activity

Artificial cerebrospinal fluid (aCSF) is commonly used to maintain the oxygen supply, osmolarity and buffer pH of isolated neurons and brain slices in electrophysiology experiments. ACSF closely matches the electrolyte concentrations of cerebrospinal fluid and is prepared from high purity water and analytical grade reagents. Microfiltered and sterile. Final ion concentrations (in mM): Na+ 150; K+ 3.0; Ca2+ 1.4; Mg2+ 0.8; P 1.0; Cl- 155.

Technical Data

Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for ACSF

The citations listed below are publications that use Tocris products. Selected citations for ACSF include:

22 Citations: Showing 1 - 10

  1. Anatomical basis and physiological role of cerebrospinal fluid transport through the murine cribriform plate.
    Authors: Norwood Et al.
    Elife  2019;8
  2. Mitochondrial Dynamin-Related Protein 1 (DRP1) translocation in response to cerebral glucose is impaired in a rat model of early alteration in hypothalamic glucose sensing.
    Authors: Desmoulins Et al.
    Mol Metab  2019;20:166
  3. Tanycyte-Independent Control of Hypothalamic Leptin Signaling.
    Authors: Yoo Et al.
    Front Neurosci  2019;13:240
  4. Ghrelin's effects on food motivation in rats are not limited to palatable foods.
    Authors: Bake Et al.
    J Neuroendocrinol  2018;:e12665
  5. Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis.
    Authors: Hill Et al.
    Br.J.Pharmacol.  2018;175:3407
  6. Acute ghrelin changes food preference from a high-fat diet to chow during binge-like eating in rodents.
    Authors: Bake Et al.
    J Neuroendocrinol  2017;29
  7. Mitochondrial Dynamics Mediated by Mitofusin 1 Is Required for POMC Neuron Glucose-Sensing and ins. Release Control.
    Authors: Ramírez Et al.
    Cell Metab  2017;25:1390
  8. Astrocytic Process Plasticity and IKKβ/NF-κB in Central Control of Blood Glucose, Blood Pressure, and Body Weight.
    Authors: Zhang Et al.
    Cell Metab  2017;25:1091
  9. SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production.
    Authors: Mitroi Et al.
    Autophagy  2017;13:885
  10. Angiotensin AT1A receptors on leptin receptor-expressing cells control resting metabolism.
    Authors: Claflin Et al.
    J Clin Invest  2017;127:1414
  11. Absence of systemic oxidative stress and increased CSF prostaglandin F2α in progressive MS
    Authors: Lam Et al.
    Neurology: Neuroimmunology & Neuroinflammation  2016;3:256
  12. The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia.
    Authors: Kurland Et al.
    J Neuroinflammation  2016;13:130
  13. DNA repair factor BRCA1 depletion occurs in Alzheimer brains and impairs cognitive function in mice.
    Authors: Suberbielle Et al.
    Front Pharmacol  2015;6:8897
  14. The depressor response to intracerebroventricular hypotonic saline is sensitive to TRPV4 antagonist RN1734.
    Authors: Feetham Et al.
    Electrophoresis  2015;6:83
  15. Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress.
    Authors: Rau Et al.
    Nat Commun  2015;35:9730
  16. Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity.
    Authors: Sartor Et al.
    J Neurosci  2015;35:15062
  17. Hypothalamic eIF2α signaling regulates food intake.
    Authors: Maurin Et al.
    Cell Rep  2014;6:438
  18. Investigating local and long-range neuronal network dynamics by simultaneous optogenetics, reverse microdialysis and silicon probe recordings in vivo.
    Authors: Taylor Et al.
    J Neurosci  2014;235:83
  19. Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics.
    Authors: McCall Et al.
    Nat Protoc  2013;8:2413
  20. Detection of cerebral spinal fluid-associated chemokines in birth traumatized premature babies by chip-based immunoaffinity CE.
    Authors: Phillips and Wellner
    J Biol Chem  2013;34:1530
  21. Central 5-α reduction of testost. is required for testosterone's inhibition of the hypothalamo-pituitary-adrenal axis response to restraint stress in adult male rats.
    Authors: Handa Et al.
    J Neurosci Methods  2013;1529:74
  22. Electrode robustness in artificial cerebrospinal fluid for dielectrophoresis-based LoC.
    Authors: Miled & Sawan
    Conf Proc IEEE Eng Med Biol Soc  2012;2012:1390


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Average Rating: 4.5 (Based on 2 Reviews)

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Using ACSF to test a protein delivery platform
By Anonymous on 11/20/2021
Species: Rat

Using ACSF to test a protein delivery platform. ACSF is ideal for modeling cerebrospinal fluid.

aCSF for in vivo application as solvent in brain infusion experiment in mice
By Jay Dela Cruz on 07/17/2017

In vivo application as solvent in brain infusion experiment in mice.

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