Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Novus Biologicals | Catalog # H00027161-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Monkey, Xenopus

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Western Blot, Immunoblotting, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E12-1C9

Format

Azide and BSA Free
View all Ago2/eIF2C2 Primary Antibodies »

Product Specifications

Immunogen

EIF2C2 (AAH07633.1, 483 a.a ~ 859 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26858302). Mouse reactivity reported in scientific literature (PMID: 28127848)

Specificity

EIF2C2 - eukaryotic translation initiation factor 2C, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Knockdown Validated: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Ago2-eIF2C2-Antibody-2E12-1C9-Western-Blot-H00027161-M01-img0018.jpg
Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Ago2-eIF2C2-Antibody-2E12-1C9-Western-Blot-H00027161-M01-img0017.jpg
Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Immunocytochemistry/ Immunofluorescence: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of monoclonal antibody to EIF2C2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H00027161-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H00027161-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

ELISA: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01]

ELISA: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.
Ago2/eIF2C2 Antibody (2E12-1C9)

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] -

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - The impact of different HAd infections on RNAi/miRNA-pathway proteins.(A) Efficiency of different HAd infections. HeLa cells were infected with the indicated viruses, followed by a 35S-methionine pulse labeling after 24 & 48 hpi. Total protein lysates were separated on an SDS-PAGE & protein synthesis visualized by autoradiography. Accumulation of late viral hexon protein is indicated by an arrow. (B) HAd infections do not affect RNAi/miRNA-pathway protein levels. Western blot analysis on the same protein samples as in panel A was used to monitor the levels of RNAi/miRNA-pathway proteins Exportin 5, Dicer, TRBP & Ago2. Detection of the Lamin B protein served as a loading control. Letter “M” denotes mock, non-infected samples. The different panels were repeated at least two times. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25144466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Ago2/eIF2C2 Antibody (2E12-1C9)

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] -

Western Blot: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - AGO2 contributed predominately to silencing activity in both regions.(A) The relative AGO mRNA levels were measured by quantitative RT-PCR 24 hours post siAgo transfection. Results were average values of assays in triplicates, & all experiments were repeated three times. (B) The relative AGO protein levels were detected by western blot 56 hours post siAgo transfection, at the time point when AGO ablated cells were harvested to evaluate luciferase activities. GAPDH was included as loading control. The intensity of protein bands was quantified by ImageJ software (NIH, USA). All experiments were performed at least twice. (C) The normalized silencing efficacies of siR-04 on perfectly matched target in CDS versus 3′-UTR after AGOs ablation. Silencing of AGO expression was carried out by gene-specific siRNA assessed in the previous study [9], & subsequently, influence of the gene silencing on perfectly-match tolerance was evaluated by reporter system. All data were normalized to mock. (D) The normalized silencing efficacies of siR-04 on single-nucleotide mismatched target sites in CDS versus 3′-UTR at the indicated positions (4C, 10U, 12G, 17A) after AGOs ablation. The target site location & siRNA:mRNA match pattern were given under the x-axis. Error bars represented SD. Data were average values of assays in triplicates, & all experiments were repeated at least twice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23145149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Ago2/eIF2C2 Antibody (2E12-1C9)

Immunoprecipitation: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] -

Immunoprecipitation: Ago2/eIF2C2 Antibody (2E12-1C9) [H00027161-M01] - Snail1 is a target of miR‐30c. (A) Sequence alignment between miR‐30c & the 3′‐UTR of Snail1 among several species. (B) Ago2 protein levels in co‐immunoprecipitated products detected by Western blot. IgGHC, IgG heavy chain; IgGLC, IgG light chain. (C) Relative expression of Snail1 in the whole RNA (left) & RNA of the nonspecific IgG or anti‐Ago2 co‐IP (right) from the HG‐treated HK2 cell lysates. #P < 0.05 vs. miR‐con + input, *P < 0.05 vs. miR‐con + IgG IP. (D) Schematic diagram of the luciferase reporter plasmids of pMIR‐Snail1 3′‐UTR & pMIR‐Snail1 3′‐UTR mut, & the potential target site of miR‐30c on the 3′‐UTR of Snail1. (E) Regulation of miR‐30c on 3′‐UTR of Snail1 in HEK293 cells by luciferase reporter assay. *P < 0.05 vs. Snail1 3′‐UTR + miR‐con. (F) Snail1 protein levels of HK2 cells with different treatments detected by Western blot. *P < 0.05 vs. NG, #P < 0.05 vs. HG + miR‐con, &P < 0.05 vs. HG + inhibitor‐con. (G) Snail1 protein levels of renal cortex detected by Western blot. *P < 0.05 vs. C57BL/Ks. #P < 0.05 vs. db/db control. (H) Stability curves of Snail1 mRNA in HG‐treated HK2 cells after transfection of miR‐30c mimics (left) or inhibitor (right). (I) The relative abundance of individual mRNA in each fraction was presented as the percentage of the total fraction following miR‐con (left) or miR‐30c (right) transfection. (J) The association of the Snail1 mRNA with putative polysome fractions (fraction 12 & fraction 13) after miR‐30c mimics transfection. Data are representative of three experiments. Data are expressed as mean ± SEM, n ≥ 3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28127848), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA and Sandwich ELISA. Chromatin Immunoprecipitation was reported in scientific literature. Use in immunprecipitation reported in scientific literature (PMID 24658750). Use in immunoblotting reported in scientific literature (PMID: 31393866).

Reviewed Applications

Read 2 reviews rated 5 using H00027161-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ago2/eIF2C2

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Alternate Names

AGO2argonaute 2, Argonaute2, EC 3.1.26.n1, EC 3.1.26.n2, eIF2C 2, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, eukaryotic translation initiation factor 2C, 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, protein argonaute-2, Protein slicer, Q10

Entrez Gene IDs

27161 (Human)

Gene Symbol

AGO2

OMIM

606229 (Human)

UniProt

AAH07633 (Human)

Additional Ago2/eIF2C2 Products

Product Documents for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

Customer Reviews for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free (2)

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  • Ago2/eIF2C2 Antibody (2E12-1C9)
    Name: Souvik Ghosh
    Application: Western Blot
    Sample Tested: mouse cell
    Species: Mouse
    Verified Customer | Posted 04/27/2018
    FACS Sorted NIH3T3 cells-Lysed in RIPA Buffer-Resolved on 10% SDS PAGE.
    NIH3T3 cells were FACS sorted based on marker gene expression.Cell lysates were compared for Ago2 expression levels
    Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free H00027161-M01
  • Ago2/eIF2C2 Antibody (2E12-1C9)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa 4
    Species: Other
    Verified Customer | Posted 04/26/2010
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Protocols

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FAQs for Ago2/eIF2C2 Antibody (2E12-1C9) - Azide and BSA Free

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  • Q: I ordered the H00027161-M01 antibody for detecting AGO2. I am using it for both IPs and westerns. The product sheet says the AGO2 should run at ~42 kDa, but online, in HL-60 cells, AGO2 runs at ~80-90 kDa, which is more of what I would expect. Can you please explain this discrepancy?

    A: Ago2 should run at around 80-90 kDa. Abnova generated their antibody against a 42 kDa partial recombinant protein. The image you see showing Ago2 at 42 kDa is from them probing the partial recombinant protein with H00027161-M01.

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