ALCAM/CD166 Antibody (4H9A5) - BSA Free

Novus Biologicals | Catalog # NBP2-37345

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4H9A5

Format

BSA Free
View all ALCAM/CD166 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ALCAM/CD166 (AA: 48-216) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALCAM/CD166 Antibody (4H9A5) - BSA Free

Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.
Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of prostate cancer tissues using ALCAM mouse mAb with DAB staining.
Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).
Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)
Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5)BSA Free [NBP2-37345]

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.
Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of bladder cancer tissues using ALCAM mouse mAb with DAB staining.
ELISA: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

ELISA: ALCAM/CD166 Antibody (4H9A5) - BSA Free [NBP2-37345]

ELISA: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for ALCAM/CD166 Antibody (4H9A5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Reviewed Applications

Read 1 review rated 5 using NBP2-37345 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALCAM/CD166

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic andhomophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as ininteractions between cells of the nervous system

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Gene Symbol

ALCAM

Additional ALCAM/CD166 Products

Product Documents for ALCAM/CD166 Antibody (4H9A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for ALCAM/CD166 Antibody (4H9A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALCAM/CD166 Antibody (4H9A5) - BSA Free

Customer Reviews for ALCAM/CD166 Antibody (4H9A5) - BSA Free (1)

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  • ALCAM/CD166 Antibody (4H9A5)
    Name: Chase Taylor
    Application: Western Blot
    Sample Tested: HT1080 human fibrosarcoma cell line
    Species: Human
    Verified Customer | Posted 03/03/2017
    See "Other experimental details"
    Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced)
    ALCAM/CD166 Antibody (4H9A5) - BSA Free NBP2-37345

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