ALCAM/CD166 Antibody (8E12C7) - BSA Free

Name: ALCAM/CD166 Antibody (8E12C7) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37358
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37358

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 8E12C7

Format

BSA Free

View all ALCAM/CD166 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ALCAM/CD166 (AA: 48-216) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALCAM/CD166 Antibody (8E12C7) - BSA Free

Western Blot: ALCAM/CD166 Antibody (8E12C7)BSA Free [NBP2-37358]

Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/ Immunofluorescence: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]

Immunocytochemistry/Immunofluorescence: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunofluorescence analysis of Hela cells using ALCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]

Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining.

Flow Cytometry: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]

Flow Cytometry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Flow cytometric analysis of JURKAT cells using ALCAM mouse mAb (green) and negative control (red).

Western Blot: ALCAM/CD166 Antibody (8E12C7)BSA Free [NBP2-37358]

Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)

Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]

Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALCAM mouse mAb with DAB staining.

ELISA: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]

ELISA: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

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Applications for ALCAM/CD166 Antibody (8E12C7) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000

Application Notes

This antibody is Cytof ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALCAM/CD166

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic andhomophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as ininteractions between cells of the nervous system

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Gene Symbol

ALCAM

Additional ALCAM/CD166 Products

Product Documents for ALCAM/CD166 Antibody (8E12C7) - BSA Free

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Certificate of Analysis

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Product Specific Notices for ALCAM/CD166 Antibody (8E12C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.