alpha-Smooth Muscle Actin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30894

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Mouse, Canine

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha-Smooth Muscle Actin. The exact sequence is proprietary.

Reactivity Notes

Use in Canine reported in scientific literature (PMID: 32330426). Predicted to show a broad reactivity.

Localization

Cytoplasm, cytoskeleton

Marker

Mesenchymal Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit alpha-Smooth Muscle Actin Antibody - BSA Free (NBP1-30894) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-alpha-Smooth Muscle Actin Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody - BSA Free

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.
Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894]

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma stroma.
alpha smooth muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Smooth Muscle Actin Antibody

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Smooth Muscle Actin Antibody

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Smooth Muscle Actin Antibody - BSA Free

Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for alpha-Smooth Muscle Actin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay-dependent dilution.

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-30894 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for alpha-Smooth Muscle Actin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Smooth Muscle Actin Antibody - BSA Free

Customer Reviews for alpha-Smooth Muscle Actin Antibody - BSA Free (2)

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  • alpha-Smooth Muscle Actin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 01/07/2021
    Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm.
    Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 1:100 Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000
    alpha-Smooth Muscle Actin Antibody - BSA Free NBP1-30894
  • alpha-Smooth Muscle Actin Antibody
    Name: Julie Kiland
    Application: Immunocytochemistry
    Sample Tested: Vascular Smooth Muscle Cells
    Species: Feline
    Verified Customer | Posted 05/18/2017
    ZEISS Axio Scope.A1. Vascular smooth muscle cells were clearly stained by NBP1-30894 alpha-SMA (Green, 1:800 dilution) and DAPI (Blue).
    alpha-Smooth Muscle Actin Antibody - BSA Free NBP1-30894

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Protocols

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FAQs for alpha-Smooth Muscle Actin Antibody - BSA Free

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    • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
      A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

    • A: NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.
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