alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488]

Name: alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488]
Brand: Novus Biologicals
SKU: NB100-690AF488
Price: 579 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NB100-690AF488

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Product Specifications
Scientific Data
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Flow Cytometry
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Background
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunomicroscopy, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # DM1A

View all alpha Tubulin Primary Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34871568) Use in Mouse reported in scientific literature (PMID:34533563). Yeast reactivity reported in scientific literature (PMID: 25126732). Goat reactivity reported in scientific literature (PMID:31805146). Will likely react with all mammals.

Marker

Microtubule Marker

Specificity

This alpha Tubulin Antibody (DM1A) does not cross-react with beta Tubulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488] [NB100-690AF488] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488] [NB100-690AF488] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488] [NB100-690AF488] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-alpha Tubulin Antibody [DM1A] conjugated to Alexa Fluor 488 (NB100-690AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow (Intracellular): alpha Tubulin Antibody (DM1A) [Alexa Fluor 488] [NB100-690AF488] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody (DM1A) NB100-690AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [Alexa Fluor 488] [NB100-690AF488] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-alpha Tubulin [DM1A] conjugated to Alexa Fluor 488 [NB100-690AF488] at 2ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Mouse Monoclonal alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488] [NB100-690AF488]

Immunohistochemistry-Paraffin: Mouse Monoclonal alpha Tubulin Antibody (DM1A) [Alexa Fluor® 488] [NB100-690AF488] - Immunofluorescence staining of human tonsil FFPE tissue in a dilution of 1:50 in 3% BSA with overnight incubation at 4°C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.

Alpha Tubulin (DM1A) in U-2 OS Human Cell Line.

Alpha Tubulin (DM1A) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-Alpha Tubulin (DM1A) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor ® 488 (Catalog # NB100-690AF488) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Detection of alpha Tubulin (DM1A) in U-2 OS Human Cell Line by Flow Cytometry.

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Mouse anti-alpha Tubulin (DM1A) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor ® 488 (Catalog # NB100-690AF488, blue histogram) or matched control antibody (orange histogram) at 5 µg/mL for 30 minutes at RT.

Alpha Tubulin (DM1A) in NIH-3T3 Mouse Cell Line.

Alpha Tubulin (DM1A) was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Mouse anti-Alpha Tubulin (DM1A) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NB100-690AF488) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

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