alpha Tubulin Antibody (DM1A) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80570

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Fungi, Goat, Guinea Pig, Hamster, Monkey, Parasite, Primate, Rabbit, Xenopus, Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunomicroscopy, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DM1A

Format

Azide and BSA Free
View all alpha Tubulin Primary Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (DM1A) - Azide and BSA Free was developed against native chicken brain microtubules.

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 25126732). Will likely react with all mammals.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

As the TUBA1A gene is conserved evolutionarily and is ubiquitously expressed in most eukaryotic cell lines, the Alpha tubulin antibody has been shown to be an attractive and effective choice for a loading control, detecting at approximately 50-55 kDa. Quantitative western blotting requires a loading control in order to account and adjust for the differences in the loading of samples across wells.

Scientific Data Images for alpha Tubulin Antibody (DM1A) - Azide and BSA Free

Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Western blot analysis of extracts from HeLa, COS and C6 cells using alpha Tubulin antibody (NB100-690, 1:1000, Alpha tubulin molecular weight: 50 kDa) Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of HeLa cells, green staining is alpha tubulin whereas red is DNA stained with propidium iodide. Image from the standard format of this antibody.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Staining of the marine parasite Cryptocaryon irritans mouth. Large bundles of microtubules form a cytophyrigeal basket. Image from the standard format of this antibody.
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using alpha Tubulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analsis of alpha tubulin in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK Image from the standard format of this antibody.
Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of alpha tubulin (molecular weight of 50 kDa) in 9 cell lysates. Lane 1. HeLa; Lane 2. JURKAT; Lane 3. COS7; Lane 4. NIH-3T3; Lane 5. PC-12; Lane 6. RAT2; Lane 7. CHO; Lane 8. MDBK; Lane 9. MDCK Image from the standard format of this antibody.
Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Western Blot: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of HeLa and COS-7 lysates. Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of embryonic fibroblasts in the anaphase portion of mitosis. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-alpha Tubulin (DM1A) (NB100-690) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - IF Confocal analysis of C6 cells using alpha Tubulin antibody (NB100-690, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Staining of skin fibroblasts. Image from the standard format of this antibody.
Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of colon tissue. Sections were formalin fixed and embedded with paraffin. Sodium citrate heat mediated antigen retrieval for 20 min. Incubated with primary antibody for 15 min at a 5 ug/ml concentration. Corner image is staining with secondary on
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of formalin fixed colon sections. Heat mediated antigen retrieval was performed using sodium citrate buffer for 20 min before incubating with primary antibody at a 0.5ug/ml dilution for 15 min at RT. Image from the standard format of this antibod
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of formalin fixed paraffin embedded heart sections. Used at a dilution of 1:500. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of paraffin embedded colon sections. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of small intestine tissue fixed with formalin and paraffin embedded showing cytoplasmic and cytoskeletal staining of glandular cells. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse prostate using alpha Tubulin Antibody (DM1A) at 1:200 dilution. The signal was developed using HRP labelled secondary and DAB reagent which followed counterstaining with hemato
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse heart using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong and specific cytoplasmic signal
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse lung using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated chunks of cytoplasmic signal in the alveol
Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse skeletal muscle using alpha Tubulin Antibody (DM1A) at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong cytoplasmic signal in
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - An intracellular stain was performed on HeLa cells with alpha Tubulin (DM1A) Antibody NB100-690JF646 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Janelia Fluor 646.
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - An intracellular stain was performed on HeLa cells with alpha Tubulin [DM1A] Antibody NB100-690AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on RAW 246.7 cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized wi Image from the standard format of this antibody.
Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570]

Flow Cytometry: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Analysis of PE conjugate of NB100-690. An intracellular stain was performed on SH-SY5Y cells with Alpha Tubulin antibody (DM1A) NB100-690PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with Image from the standard format of this antibody.
Simple Western: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Simple Western: alpha Tubulin Antibody (DM1A)Azide and BSA Free [NBP2-80570]

Simple Western: alpha Tubulin Antibody (DM1A) - Azide and BSA Free [NBP2-80570] - Simple Western lane view shows a specific band for alpha Tubulin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.

Applications for alpha Tubulin Antibody (DM1A) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:50000-1:100000

Immunohistochemistry-Frozen

1:100-1:500

Immunoprecipitation

1:50-1:100

Simple Western

1:50

Western Blot

1:5000
Application Notes
This alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry.
The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining.

This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T.,... Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Gene Symbol

TUBA1A

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (DM1A) - Azide and BSA Free

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Product Specific Notices for alpha Tubulin Antibody (DM1A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

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