ALS2CR2 Antibody

Novus Biologicals | Catalog # NB100-1181

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ALS2CR2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CDFPDEKDSYWEF corresponding to C-Terminus according to NP_061041.2, AAF71042.1.

Specificity

This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ALS2CR2 Antibody

Western Blot: ALS2CR2 Antibody [NB100-1181]

Western Blot: ALS2CR2 Antibody [NB100-1181]

Western Blot: ALS2CR2 Antibody [NB100-1181] - HEK293 overexpressing ILPIP and probed with (mock transfection in first lane).
Immunocytochemistry/ Immunofluorescence: ALS2CR2 Antibody [NB100-1181]

Immunocytochemistry/ Immunofluorescence: ALS2CR2 Antibody [NB100-1181]

Immunocytochemistry/Immunofluorescence: ALS2CR2 Antibody [NB100-1181] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry: ALS2CR2 Antibody [NB100-1181]

Immunohistochemistry: ALS2CR2 Antibody [NB100-1181]

Immunohistochemistry: ALS2CR2 Antibody [NB100-1181] - Staining of paraffin embedded Human Heart. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ALS2CR2 Antibody [NB100-1181]

Western Blot: ALS2CR2 Antibody [NB100-1181]

Western Blot: ALS2CR2 Antibody [NB100-1181] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ALS2CR2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 35 kDa band observed in human heart, liver and muscle lysates (calculated MW of 47.0 kDa band according to NP_061041.2 (alpha isoform) and 31.2 kDa band according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human heart shows staining of myocardial fibres in transverse section.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALS2CR2

Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, is connected to transferase/kinase activity and ATP binding, it has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family. Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1beta converting enzyme of Fas-induced apotosis. This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival, suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

Alternate Names

ALS2CR2candidate 2, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein, CALS-21MGC102916, ILP-interacting protein, ILPIPSTRAD beta, Pseudokinase ALS2CR2, STE20-related kinase adaptor beta

Entrez Gene IDs

55437 (Human)

Gene Symbol

STRADB

UniProt

AAF71042 (Human)

Additional ALS2CR2 Products

Product Documents for ALS2CR2 Antibody

Certificate of Analysis

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Product Specific Notices for ALS2CR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALS2CR2 Antibody

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Protocols

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