AMPK alpha 2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-56322
![Western Blot: AMPK alpha 2 Antibody [NBP1-56322]](https://resources.rndsystems.com/images/products/AMPK-alpha-2-Antibody-Western-Blot-NBP1-56322-img0004.jpg "Western Blot: AMPK alpha 2 Antibody [NBP1-56322]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide directed towards the middle region of human PRKAA2 (NP_006243). Peptide sequence: AYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPP. The peptide sequence for this immunogen was taken from within the described region.
Specificity
This product is specific to Subunit or Isoform: alpha-2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for AMPK alpha 2 Antibody - BSA Free
Western Blot: AMPK alpha 2 Antibody [NBP1-56322]
Western Blot: AMPK alpha 2 Antibody [NBP1-56322] - Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate.![Western Blot: AMPK alpha 2 Antibody [NBP1-56322]](https://resources.rndsystems.com/images/products/AMPK-alpha-2-Antibody-Western-Blot-NBP1-56322-img0002.jpg "Western Blot: AMPK alpha 2 Antibody [NBP1-56322]")
Western Blot: AMPK alpha 2 Antibody [NBP1-56322]
Western Blot: AMPK alpha 2 Antibody [NBP1-56322] - Sample Type: rat hepatocyte (50ug) Priamry Dilution: 1:4000 Secondary Antibody: Donkey anti-Rabbit HRP Secondary Dilution: 1:10,000 Image Submitted By: Dustin Singer, INRA/AgroParistech![Western Blot: AMPK alpha 2 Antibody [NBP1-56322]](https://resources.rndsystems.com/images/products/AMPK-alpha-2-Antibody-Western-Blot-NBP1-56322-img0003.jpg "Western Blot: AMPK alpha 2 Antibody [NBP1-56322]")
Western Blot: AMPK alpha 2 Antibody [NBP1-56322]
Western Blot: AMPK alpha 2 Antibody [NBP1-56322] - Middle region validated by WB using Rat Liver, Human Muscle, Rat Muscle, Mouse Muscle at 1:1000.Applications for AMPK alpha 2 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1.0 ug/ml
Application Notes
Use in IHC reported in scientific literature (PMID:34536344).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AMPK alpha 2
Long Name
AMP-activated Protein Kinase alpha
Alternate Names
PRKAA2
Entrez Gene IDs
5563 (Human)
Gene Symbol
PRKAA2
UniProt
Additional AMPK alpha 2 Products
Product Documents for AMPK alpha 2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AMPK alpha 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for AMPK alpha 2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
