Recombinant Mouse, Porcine Angiopoietin-4 Protein

Novus Biologicals | Catalog # NBP3-57469

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all Angiopoietin-4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 120 aa of Mouse,Porcine Angiopoietin-4.

Source: Yeast

Amino Acid Sequence: QNERYEKFLR QHYDAKPNGR DDRYCESMMK ERKLTSPCKD VNTFIHGTKK NIRAICGKKG SPYGENFRIS NSPFQITTCT HSGASPRPPC GYRAFKDFRY IVIACEDGWP VHFDESFISP (120)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

13.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57469
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-4

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Alternate Names

ANGPT4

Gene Symbol

ANGPT4

Additional Angiopoietin-4 Products

Product Documents for Recombinant Mouse, Porcine Angiopoietin-4 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse, Porcine Angiopoietin-4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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