Annexin V Antibody (1F4-1A5) - Azide and BSA Free

Novus Biologicals | Catalog # H00000308-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F4-1A5

Format

Azide and BSA Free
View all Annexin V Primary Antibodies »

Product Specifications

Immunogen

ANXA5 (AAH01429, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD

Specificity

ANXA5 - annexin A5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody (M01), clone 1F4-1A5.Lane 1: ANXA5 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunocytochemistry/Immunofluorescence: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of monoclonal antibody to ANXA5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunohistochemistry-Paraffin: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunohistochemistry-Paraffin: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01] - ANXA5 monoclonal antibody (M01), clone 1F4-1A5. Analysis of ANXA5 expression in HeLa.
Immunoprecipitation: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunoprecipitation: Annexin V Antibody (1F4-1A5) [H00000308-M01]

Immunoprecipitation: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of ANXA5 transfected lysate using anti-ANXA5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ANXA5 MaxPab rabbit polyclonal antibody.
ELISA: Annexin V Antibody (1F4-1A5) [H00000308-M01]

ELISA: Annexin V Antibody (1F4-1A5) [H00000308-M01]

ELISA: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Detection limit for recombinant GST tagged ANXA5 is approximately 0.03ng/ml as a capture antibody.

Applications for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

3 ug/ml

Simple Western

1:25

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Annexin V

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Alternate Names

ANX5, ANXA5, ANXV

Entrez Gene IDs

308 (Human)

Gene Symbol

ANXA5

OMIM

131230 (Human)

UniProt

AAH01429 (Human)

Additional Annexin V Products

Product Documents for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Coagulation

Citations for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Annexin V Antibody (1F4-1A5) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

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