Anti-Trastuzumab (Anti-Idiotype) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Trastuzumab by Western Blot. Western blot showsTrastuzumab, Adalimumab, Brentuxumab, and Gemtuzumab biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Trastuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Trastuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Trastuzumab (Anti-Idiotype) Antibody in ELISA Capture Assay. Biotinylated biosimilars were serially diluted and incubated with Anti-Trastuzumab (Anti-Idiotype) Antibody (Catalog # MAB9547, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Adalimumab (black line) or Gemtuzumab (orange line) was observed.
Specificity of Anti-Trastuzumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Trastuzumab (Anti-Idiotype) Antibody (Catalog # MAB9547, 333 ng/mL) detected Trastuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
What is an anti-idiotype antibody?
An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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