APE Antibody (13B8E5C2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80578

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Proximity Ligation Assay, Gel Super Shift Assays, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 13B8E5C2

Format

Azide and BSA Free
View all APE Primary Antibodies »

Product Specifications

Immunogen

Purified human APE1 [Uniprot: P27695]

Epitope

The epitope is within amino acids 80-100.

Localization

In immunohistochemical experiments punctate nuclear staining is observed in most cell types. Cytoplasmic staining may be seen in hepatocytes, large neurons and granule cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APE Antibody (13B8E5C2) - Azide and BSA Free

Western Blot: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Immunohistochemical staining of APE1. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from
Immunohistochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunohistochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunohistochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Chromatin Immunoprecipitation: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Association of p53 and APE1 on p53-binding sites in p21 promoter. Re-ChIP analysis (first IP with alpha-APE1 and the second IP with alpha-p53 antibody) showing simultaneous recruitment of APE1 and p53 in control vs. EPE treated cells; *: p value <0.05 (n=2) calculated based on APE1/p53 enriched DNA from control vs. etoposide treated cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license. Image from the standard format of this antibody.
Western Blot: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Ovarian Cancer cell lines. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry/ Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Immunocytochemistry: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDA MB 231. Image from the standard format of this antibody.
Simple Western: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Simple Western: APE Antibody (13B8E5C2)Azide and BSA Free [NBP2-80578]

Simple Western: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Image shows a specific band for APE1 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from the standard format of this antibody.
Knockdown Validated: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578]

Western Blot: APE Antibody (13B8E5C2) - Azide and BSA Free [NBP2-80578] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1, p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license. Image from the standard format of this antibody.

Applications for APE Antibody (13B8E5C2) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

reported in scientific literature

Immunoblotting

reported in scientific literature (PMID 27608656)

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Proximity Ligation Assay

reported in scientific literature (PMID 27808278)

Simple Western

1:25

Western Blot

1:100 - 1:2000
Application Notes
In Western blot, this antibody detects a single band at 37 kDa. In IHC, it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. It can also be used on frozen and fixed-paraffin sections and cytospin preps. In IHC-P, staining was observed in the nucleus of a human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:25. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol

APEX1

Additional APE Products

Product Documents for APE Antibody (13B8E5C2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for APE Antibody (13B8E5C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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