Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Novus Biologicals | Catalog # NB400-147

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, SDS-Page

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Apolipoprotein A-I/ApoA1 Primary Antibodies »

Product Specifications

Immunogen

Apolipoprotein A-I/ApoA1 was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat. (Uniprot: P02647)

Reactivity Notes

Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Localization

Secreted

Specificity

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NB400-147-img0004.jpg
Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147]

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Coomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apolipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.
Apolipoprotein A-I/ApoA1 Antibody

Apolipoprotein A-I/ApoA1 Antibody

anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Apolipoprotein A-I/ApoA1 Antibody

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] -

Cross-linking analysis of fmHDL apolipoprotein. fmHDL (apo A-I), fmHDL (apo A-I-SH), and fmHDL (no apo A-I) were cross-linked with BS3. The apolipoprotein cross-link products were resolved on a 4–20% SDS PAGE gel and visualized by apo A-I immunoblotting. An oligomer ladder was prepared with lipid-free apo A-I (apo A-I lane) for cross-link product identification. fmHDL migrated on the PAGE gel as a clearly visible band and were detectible by apo A-I immunoblotting (marked as fmHDL on the left).

Applications for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:20000

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:1000-1:2000
Application Notes
This product have been tested by Western blot and IHC and are suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.

Use in Sandwich-ELISA reported in scientific literature (PMID 26648662)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Entrez Gene IDs

335 (Human)

Gene Symbol

APOA1

UniProt

P02647

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Obesity

Citations for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

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Protocols

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