Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 960319

Format

Azide and BSA Free
View all Apolipoprotein E/ApoE Primary Antibodies »

Product Specifications

Immunogen

Apolipoprotein E/ApoE variant peptide found within amino acids 100-200.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free (MAB41442) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free

Immunocytochemistry: Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free [MAB41442]

Immunocytochemistry: Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free [MAB41442]

Apolipoprotein E/ApoE was detected in fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41442) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry: Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free [MAB41442]

Immunohistochemistry: Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free [MAB41442]

Apolipoprotein E/ApoE was detected in immersion fixed paraffin-embedded sections of human brain hippocampus using Mouse Monoclonal Apolipoprotein E/ApoE Antibody (960319) at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm.

Applications for Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apolipoprotein E/ApoE Antibody (960319) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

A-beta Pathways: Uptake & Degradation A-beta Pathways: Uptake & Degradation Thumbnail