Aquaporin 1/AQP1 Antibody (1/A5F6)

Novus Biologicals | Catalog # NB600-749

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1/A5F6
View all Aquaporin 1/AQP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 249-269 of aquaporin 1

Reactivity Notes

Predicted cross-reactivities: Rat, Rabbit, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Plasma membrane

Specificity

Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Aquaporin 1/AQP1 Antibody (1/A5F6)

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0002.jpg
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0001.jpg
Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749]

Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0003.jpg
Aquaporin 1/AQP1 Antibody (1/A5F6)

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] -

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys & urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies & merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) & distal (THP) renal tubular epithelial cells & renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder & left & right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) & uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) & merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aquaporin 1/AQP1 Antibody (1/A5F6)

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] -

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - USSC administration recovered aquaporin1 (AQP1) expression in the choroid plexus after PHH. (A) Representative immunofluorescence image of cryosections labeled with AQP1 specific antibody (arrows showing choroid epithelium immunoreactivity) on postnatal days 7. Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & images from left to right respectively at postnatal day 7. CPE (Choroid plexus epithelial cells). Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (B) Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & the images from left to right respectively at day 14. Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (C) Representative immunofluorescence image of cryosections labeled with aquaporin1. As seen in the high power image (panel-B above), the strong AQP1 immunoreactivity is evident over the entire choroid plexus in the control, reduced signal in IVH followed by recovered strong AQP1 expression observed in images from left to right respectively at day 14. Images were taken at 4× objective; 20 μm sections. The scale bar is 500 μm. Fluorescence images were taken using “Nikon Eclipse 90i microscope” (Nikon Instruments, Japan). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33897371), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aquaporin 1/AQP1 Antibody (1/A5F6)

Western Blot: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] -

AQP1and AQP3 protein expression in disc degeneration. (A) Representative microscopic photographs of immunofluorescence staining showing AQP1 and AQP3 immunoreaction-positive products. Nuclei counterstained with DAPI showed blue, with AQP1 and AQP3 positive products appearing green. Scale bar: 20 μm. The average optical density (AOD) was measured. (B,C) Bar graphs showing the AQP1 and AQP3 immunoreaction-positive products. (D) Western blot analysis of AQP1 and AQP3 protein levels. GAPDH was analyzed as a housekeeping gene. (E, F) Bar graphs showing the protein levels of AQP1 and AQP3. (G, H) Bar graphs showing the mRNA levels of AQP1 and AQP3. Data are expressed as mean +/- SD; **P < 0.01 compared to the sham compressed group; ##P < 0.01 and #P < 0.05 compared to the model group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40128722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aquaporin 1/AQP1 Antibody (1/A5F6)

Application
Recommended Usage

ELISA

1:1000-1:20000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:1000-1:5000
Application Notes
ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review.

Reviewed Applications

Read 1 review rated 4 using NB600-749 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 1/AQP1

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)

Alternate Names

AQP1, CHIP28, CO

Entrez Gene IDs

358 (Human); 11826 (Mouse); 25240 (Rat)

Gene Symbol

AQP1

UniProt

P29972 (Human), P29975 (Rat)

Additional Aquaporin 1/AQP1 Products

Product Documents for Aquaporin 1/AQP1 Antibody (1/A5F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Aquaporin 1/AQP1 Antibody (1/A5F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

Citations for Aquaporin 1/AQP1 Antibody (1/A5F6)

Customer Reviews for Aquaporin 1/AQP1 Antibody (1/A5F6) (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Rat Lung Tissue
    Species: Rat
    Verified Customer | Posted 11/30/2010
    Aquaporin 1/AQP1 Antibody (1/A5F6) NB600-749

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Aquaporin 1/AQP1 Antibody (1/A5F6)

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  • Q: Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.

    A:

    A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

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