The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in intestinal brush border membranes, but is also expressed in the kidney, brain and liver. ASAH2 is involved in the metabolism of dietary sphingolipids and the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-49326
![Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.rndsystems.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Western-Blot-NBP2-49326-img0003.jpg "Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQNHQT
Reactivity Notes
Mouse (85%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Western Blot: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
![Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.rndsystems.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49326-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
Immunocytochemistry/ Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Immunocytochemistry/Immunofluorescence: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.![Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]](https://resources.rndsystems.com/images/products/ASAH2-N-acylsphingosine-Amidohydrolase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49326-img0002.jpg "Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]")
Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326]
Immunohistochemistry-Paraffin: ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [NBP2-49326] - Staining of human duodenum shows luminal membranous positivity in glandular cells.Applications for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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