ATF2 Antibody (3D12) - BSA Free

Name: ATF2 Antibody (3D12) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26498
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26498

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3D12 expressed in HEK293

Format

BSA Free

View all ATF2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ATF2 [UniProt P15336]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF2 Antibody (3D12) - BSA Free

Immunohistochemistry: ATF2 Antibody (3D12) [NBP3-26498] -

Immunohistochemistry: ATF2 Antibody (3D12) [NBP3-26498] - Image of ATF2 Antibody (3D12) diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: ATF2 Antibody (3D12) [NBP3-26498] -

Western Blot: ATF2 Antibody (3D12) [NBP3-26498] - Positive Western Blot detected in: K562 whole cell lysate, 293T whole cell lysate.
All lanes: ATF2 Antibody at 1.2ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
Observed band size: 70 KDa

Applications for ATF2 Antibody (3D12) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 Antibody (3D12) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATF2 Antibody (3D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.