ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

Name: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-14001
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14001

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # PCRP-ATF2-1B4

Format

Azide and BSA Free

View all ATF2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ATF2 protein (Uniprot: P15336)

Reactivity Notes

Predicted to react in mouse and rat.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

54.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13855).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

Protein Array: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001]

Protein Array: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ATF2 Antibody (PCRP-ATF2-1B4).

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] -

Flow Cytometric Analysis of PFA-fixed U87cells. ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] -

Immunofluorescence Analysis of HeLacells stained using ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] -

Flow Cytometric Analysis of PFA-fixed HeLacells. ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] -

Immunofluorescence Analysis of HeLacells stained using ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

View 2 more images

Applications for ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.