ATG12 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-15501
![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0015.jpg "Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Predicted:
Chimpanzee (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.
Reactivity Notes
Pig (81%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG12 Antibody - BSA Free
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Western-Blot-NBP2-15501-img0002.jpg "Western Blot: ATG12 Antibody [NBP2-15501]")
Western Blot: ATG12 Antibody [NBP2-15501]
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.![Western Blot: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Western-Blot-NBP2-15501-img0011.jpg "Western Blot: ATG12 Antibody [NBP2-15501]")
![Western Blot: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Western-Blot-NBP2-15501-img0014.jpg "Western Blot: ATG12 Antibody [NBP2-15501]")
Western Blot: ATG12 Antibody [NBP2-15501]
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Immunohistochemistry-Paraffin-NBP2-15501-img0008.jpg "Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]")
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501]
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunoprecipitation: ATG12 Antibody [NBP2-15501]](https://resources.rndsystems.com/images/products/ATG12-Antibody-Immunoprecipitation-NBP2-15501-img0006.jpg "Immunoprecipitation: ATG12 Antibody [NBP2-15501]")
Immunoprecipitation: ATG12 Antibody [NBP2-15501]
Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Western Blot: ATG12 Antibody [NBP2-15501] -
Western Blot: ATG12 Antibody [NBP2-15501] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for ATG12 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ATG12
Long Name
ATG12 Autophagy Related 12 Homolog
Alternate Names
APG12, FBR93, HAPG12
Gene Symbol
ATG12
Additional ATG12 Products
Product Documents for ATG12 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATG12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ATG12 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ATG12 Antibody - BSA Free
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Q: I used ATG12 antibody [NBP2-15501] to detect ATG12 and ATG12-ATG5 complex in rat myocardium tissue lysates by Western blot. In results, I found two bands, one was just below the 55kD, another was right at 15kD, very different from the images on our website. I do not see any article about its molecular weight, can you plaease confirm if the bands are of ATG12 or ATG12-ATG5? May I ask if you got any data on rat myocardium or any references?
A: Rat's ATG12 has a predicted MW of 15 kDa, so I think you have the right band for that. ATG12-ATG5 complex in rat samples will not run exactly as it does in human (band slightly above 55 kDa). At the moment, we are not aware of any publications that show the ATG12-ATG5 complex on a WB.You may consider using both an ATG5 and ATG12 antibody on their samples to see if the complex is detected with two different antibodies. Alternatively you can use KO lysates or siRNA to knockdown ATG5 or ATG12.
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